{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T14:06:33Z","timestamp":1789221993381,"version":"build-2803163510"},"reference-count":29,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clin Appl Thromb Hemost"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Circulating microRNAs may predict stroke outcomes, but unaccounted sampling time can distort their prognostic value. We assessed whether plasma miR-424-5p interpreted with the elapsed time from stroke onset to blood draw predicted early neurological deterioration (END) and 90-day disability after acute ischemic stroke.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This prospective single-center observational cohort enrolled consecutive adults with imaging-confirmed acute ischemic stroke in China from June 2024 to June 2025. Time zero was the first research plasma draw with contemporaneous baseline NIHSS assessment. Primary analyses evaluated post-baseline END within 72 hours and 90-day disability in patients with valid repeat sampling 18\u201330 hours after time zero. Baseline miR-424-5p and 24-hour change normalized to the actual interval between the two blood draws were tested using multivariable models adjusted for pretreatment\/time-zero covariates and onset-to-draw windows, with bootstrap internal validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among 368 patients, 73 developed END (19.8%). The repeat-sample cohort included 337 patients, of whom 149 had 90-day disability (44.2%). Higher baseline miR-424-5p independently predicted END (adjusted OR per 1 SD, 1.57; 95% CI, 1.16\u20132.13; p=0.003). For 90-day disability, normalized 24-hour miR-424-5p change, but not baseline level, remained independently associated with outcome (adjusted OR per 1 SD, 1.69; 95% CI, 1.24\u20132.31; p=0.001). Within this development dataset, biomarker addition yielded apparent AUC changes from 0.79 to 0.83 for END and from 0.80 to 0.84 for disability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Time-anchored baseline miR-424-5p predicted post-baseline END, whereas its normalized 24-hour trajectory predicted 90-day disability. External validation, including comparison with 24-hour neurological reassessment, is needed.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10760296261489115","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:04:18Z","timestamp":1789059858000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Time-Anchored miR-424-5p Predicts Neurological Deterioration and 90-Day Disability After Acute Ischemic Stroke"],"prefix":"10.1177","volume":"32","author":[{"given":"Zhao","family":"Li","sequence":"first","affiliation":[{"name":"Taixing People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weixiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Taixing People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Tang","sequence":"additional","affiliation":[{"name":"Taixing People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rujuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Taixing People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jikai","family":"Yin","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Nantong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1356-0449","authenticated-orcid":false,"given":"Qiaozhuan","family":"Li","sequence":"additional","affiliation":[{"name":"Taixing People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(24)00369-7"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.4103\/bc.bc_198_25"},{"key":"e_1_3_7_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/svn-2023-002998"},{"key":"e_1_3_7_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/17562864221147743"},{"key":"e_1_3_7_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2026.1737871"},{"key":"e_1_3_7_7_2","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S365758"},{"key":"e_1_3_7_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000543763"},{"key":"e_1_3_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.70577"},{"key":"e_1_3_7_10_2","doi-asserted-by":"publisher","DOI":"10.2144\/fsoa-2023-0142"},{"key":"e_1_3_7_11_2","doi-asserted-by":"publisher","DOI":"10.20517\/2574-1209.2025.58"},{"key":"e_1_3_7_12_2","doi-asserted-by":"publisher","DOI":"10.5853\/jos.2020.05085"},{"key":"e_1_3_7_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X221085090"},{"key":"e_1_3_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clineuro.2024.108416"},{"key":"e_1_3_7_15_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.83963"},{"key":"e_1_3_7_16_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S340586"},{"key":"e_1_3_7_17_2","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2017.0602"},{"key":"e_1_3_7_18_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10888"},{"key":"e_1_3_7_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311572"},{"key":"e_1_3_7_20_2","doi-asserted-by":"publisher","DOI":"10.1136\/svn-2024-003977"},{"key":"e_1_3_7_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.20.7.864"},{"key":"e_1_3_7_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.19.5.604"},{"key":"e_1_3_7_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaf043"},{"key":"e_1_3_7_24_2","doi-asserted-by":"publisher","unstructured":"Van Calster B McLernon DJ van Smeden M Wynants L Steyerberg EW. 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