{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:07:49Z","timestamp":1777932469020,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:p>\n                    We investigated fecal colonization with third-generation cephalosporin-resistant (3GC-r) Enterobacterales and carbapenem-resistant Enterobacterales among food animals and their handlers in Ghana. A total of 252 fecal samples were collected from 211 animals and 41 human handlers across 20 farms between May and August 2023. Enterobacterales were isolated using standard methods and identified using Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry fingerprints and 16SrRNA sequencing. Antibiotic susceptibility testing was done using standard methods. Extended spectrum beta-lactamase (ESBL) and carbapenemase genes were identified by PCR and sequencing. A total of 264 Enterobacterales were isolated, comprising 44 from human handlers and 220 from food animals. Among human isolates, 31 (70.5%) were 3GC-r, with 9 (20.5%) expressing the ESBL phenotype and 2 (4.5%) producing carbapenemases. The most common ESBL genes detected were\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 5\/9) and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-14<\/jats:sub>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 2\/9), while carbapenemase-producing isolates harbored\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-1<\/jats:sub>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 1\/2) or\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>IMP-1<\/jats:sub>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 1\/2). In food animals, 94 (42.7%) of Enterobacterales isolates were 3GC-r, with 11 (5.0%) carrying ESBL genes, predominantly\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 5\/11). One\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolate exhibited carbapenemase production (\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-1<\/jats:sub>\n                    ) with an ESBL gene (\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-1<\/jats:sub>\n                    ). Meropenem was the most effective antibiotic agent against the study isolates (\u22645% resistance). Concordance of isolate\/resistance gene combinations was observed at three farms where at least one human handler and at least one farm animal carried the same 3GC-r\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    with the same resistance genes, including\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>TEM-10<\/jats:sub>\n                    , and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-1.<\/jats:sub>\n                    The findings indicate a potential for zoonotic transmission of resistance genes between food animals and their human handlers.\n                  <\/jats:p>","DOI":"10.1177\/10766294251360947","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:11:13Z","timestamp":1759943473000},"page":"231-240","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Carriage of Extended Spectrum Beta-Lactamase and Carbapenemase Genes in Food Animals and Their Human Handlers: A One Health Perspective in a Low-Resource Setting in Africa"],"prefix":"10.1177","volume":"31","author":[{"given":"Sarah","family":"Saidu","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akua","family":"Obeng Forson","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Appiah-Kwarteng","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Oduro-Mensah","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Molecular Biology, School of Biological Sciences, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Olu-Taiwo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjorie","family":"Ntiwaa Quarchie","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary-Magdalene","family":"Osei","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Kwao","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noah","family":"Obeng-Nkrumah","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/soc14090187"},{"key":"e_1_3_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-024-03318-x"},{"key":"e_1_3_7_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-024-02267-8"},{"key":"e_1_3_7_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14020141"},{"key":"e_1_3_7_6_2","volume-title":"Global Molecular Characterization of Extended-Spectrum \u00df-lactamases and carbapenemases in Enterobacterales","author":"Lascols C","year":"2024","unstructured":"Lascols C. Global Molecular Characterization of Extended-Spectrum \u00df-lactamases and carbapenemases in Enterobacterales. Universit\u00e9 Paris-Saclay; 2024."},{"issue":"6","key":"e_1_3_7_7_2","first-page":"e06651","article-title":"Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain","volume":"19","author":"EPoB H","year":"2021","unstructured":"EPoB H, , Koutsoumanis K, , Allende A, et al. Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain. EFSA Journal, 2021; 19(6):e06651.","journal-title":"EFSA Journal"},{"key":"e_1_3_7_8_2","unstructured":"\u0394\u03b9\u03bf\u03bb\u03ae \u03a7. Molecular epidemiology and antimicrobial resistance spread of environmental Escherichia coli isolates: A one health approach. 2024."},{"key":"e_1_3_7_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12091920"},{"key":"e_1_3_7_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-023-01113-z"},{"key":"e_1_3_7_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13111087"},{"key":"e_1_3_7_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13070662"},{"key":"e_1_3_7_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0301531"},{"key":"e_1_3_7_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2022.0060"},{"key":"e_1_3_7_15_2","unstructured":"Report FHA. Family Health Annual Report. Ghana Health Service Accra: Ghana; 2014."},{"key":"e_1_3_7_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0310058"},{"key":"e_1_3_7_17_2","unstructured":"CLISI. Clinical and Laboratory Standards Institute. 2023."},{"key":"e_1_3_7_18_2","unstructured":"Clinical LSI. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 31 editor. 2021."},{"key":"e_1_3_7_19_2","first-page":"3990","article-title":"Brief outline about biofilm resistance to antimicrobials","author":"Boghdady WAMH","year":"2023","unstructured":"Boghdady WAMH, , Asaad AMM, , Tantawy EA, et al. Brief outline about biofilm resistance to antimicrobials. Tobacco Regulatory Science (TRS), 2023:3990\u20134010.","journal-title":"Tobacco Regulatory Science (TRS)"},{"key":"e_1_3_7_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-019-1629-x"},{"key":"e_1_3_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2023.115918"},{"key":"e_1_3_7_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12061016"},{"key":"e_1_3_7_23_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27134151"},{"key":"e_1_3_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfp.2023.100108"},{"key":"e_1_3_7_25_2","unstructured":"\u00d8deskaug LE Danielsen AS Tornes RA et al. Key factors in screening for extended-spectrum beta-lactamase (ESBL)-producing bacteria and carbapenemase-producing organism (CPO): A narrative synthesis of current evidence. 2025."},{"key":"e_1_3_7_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12010144"},{"key":"e_1_3_7_27_2","unstructured":"Lamperti L. Detection of carbapenem-resistant Enterobacterales in food producing animals and human patients: A\u201d One Health\u201d perspective. 2024."},{"key":"e_1_3_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.101651"},{"key":"e_1_3_7_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03316-22"},{"key":"e_1_3_7_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12010001"},{"key":"e_1_3_7_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlab092"},{"key":"e_1_3_7_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13040295"},{"key":"e_1_3_7_33_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11092127"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10766294251360947","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10766294251360947","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10766294251360947","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T01:20:31Z","timestamp":1777684831000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10766294251360947"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,30]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,1]]}},"alternative-id":["10.1177\/10766294251360947"],"URL":"https:\/\/doi.org\/10.1177\/10766294251360947","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,30]]}}}