{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:54:03Z","timestamp":1792806843364,"version":"4.5.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>\n                    In high-burden tuberculosis (TB) settings such as Iran, non-tuberculous mycobacteria (NTM) are increasingly identified among presumptive TB cases. However, their epidemiology and drug resistance patterns remain inadequately described. This study investigated the prevalence, species distribution, and antimicrobial susceptibility of NTM isolates from 3,000 clinical specimens collected from patients with presumptive TB at the Pasteur Institute of Iran between March 2022 and March 2023. Identification was performed through culture and sequencing of the\n                    <jats:italic toggle=\"yes\">16S rDNA<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">hsp65<\/jats:italic>\n                    genes. Drug susceptibility testing (DST) was conducted using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute guidelines. Among 145 acid-fast bacilli-positive cultures, 45 (31%) were identified as NTM. The predominant species were\n                    <jats:italic toggle=\"yes\">Mycobacterium fortuitum<\/jats:italic>\n                    (51.1%) and\n                    <jats:italic toggle=\"yes\">M. simiae<\/jats:italic>\n                    (40.0%), followed by less common isolates of\n                    <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">M. kansasii<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">M. flavescens<\/jats:italic>\n                    . The majority of NTM isolates (86.7%) originated from respiratory specimens. Phenotypic analyses revealed high resistance rates to first-line anti-TB drugs such as isoniazid and rifampicin, while susceptibility varied across fluoroquinolones, aminoglycosides, and sulfonamides. These findings underscore the importance of species-level identification and DST-guided therapy to improve the clinical management of NTM infections in TB-endemic regions.\n                  <\/jats:p>","DOI":"10.1177\/10766294251375421","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T13:10:28Z","timestamp":1759842628000},"page":"303-308","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Epidemiology of Non-Tuberculous Mycobacteria Among Tuberculosis-Suspected Patients in Iran: Species Distribution and Drug Resistance"],"prefix":"10.1177","volume":"31","author":[{"given":"Maryam","family":"Rahimi","sequence":"first","affiliation":[{"name":"Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Akhavan Sepahi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Sakhaee","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Davar","family":"Siadat","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran."},{"name":"Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abolfazl","family":"Fateh","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran."},{"name":"Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,10,6]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2022.10.013"},{"issue":"4","key":"e_1_3_6_3_2","first-page":"1557","article-title":"Identification of non-tuberculous Mycobacteria clinical isolates to species level using four different advanced diagnostic technologies","volume":"5","author":"Soliman MS","year":"2024","unstructured":"Soliman MS, , Soliman NS, , Abbas AM, et al. Identification of non-tuberculous Mycobacteria clinical isolates to species level using four different advanced diagnostic technologies. Microbes Infect Dis, 2024; 5(4):1557\u20131566.","journal-title":"Microbes Infect Dis"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e35311"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.36497\/respirsci.v4i3.122"},{"key":"e_1_3_6_6_2","unstructured":"Maleknia M Ayoubi S Dezhkhi H et al. Epidemiology of temporal trends drug resistance and effective factors in mycobacterial infections: A seven-year analysis in Masih Daneshvari Hospital. 2024."},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijtb.2024.08.006"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12081615"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e28665"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-09016-6"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkad317"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40506-016-0086-4"},{"issue":"2","key":"e_1_3_6_13_2","first-page":"96","article-title":"Drug resistance in nontuberculous mycobacteria: Mechanisms and models","volume":"10","author":"Saxena S","year":"2021","unstructured":"Saxena S, , Spaink HP, , Forn-Cun\u00ed G. Drug resistance in nontuberculous mycobacteria: Mechanisms and models. Biology (Basel), 2021; 10(2):96.","journal-title":"Biology (Basel)"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00516-19"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1104752"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-022-02674-z"},{"issue":"4","key":"e_1_3_6_17_2","first-page":"e30763","article-title":"Phylogenetic analysis of prevalent tuberculosis and non-tuberculosis mycobacteria in Isfahan, Iran, based on a 360 bp sequence of the rpoB gene","volume":"9","author":"Esfahani BN","year":"2016","unstructured":"Esfahani BN, , Moghim S, , Safaei HG, et al. Phylogenetic analysis of prevalent tuberculosis and non-tuberculosis mycobacteria in Isfahan, Iran, based on a 360 bp sequence of the rpoB gene. Jundishapur J Microbiol, 2016; 9(4):e30763.","journal-title":"Jundishapur J Microbiol"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.4103\/2277-9175.217216"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijregi.2024.100464"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1477015"},{"key":"e_1_3_6_21_2","unstructured":"Woods GL Brown-Elliott BA Conville PS et al. Susceptibility testing of mycobacteria nocardiae and other aerobic actinomycetes. 2011."},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-023-01557-4"},{"issue":"3","key":"e_1_3_6_23_2","first-page":"e81017","article-title":"Extrapulmonary nontuberculous mycobacteria infection: The new-age neglected infectious disease","volume":"17","author":"Rath S","year":"2025","unstructured":"Rath S, , Firdaus S, , Nayak G, et al. Extrapulmonary nontuberculous mycobacteria infection: The new-age neglected infectious disease. Cureus, 2025; 17(3):e81017.","journal-title":"Cureus"},{"key":"e_1_3_6_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000004506"},{"issue":"1","key":"e_1_3_6_25_2","first-page":"75","article-title":"Incidence, clinical manifestation, treatment outcome, and drug susceptibility pattern of nontuberculous mycobacteria in HIV patients in Tehran, Iran","volume":"30","author":"Feysia SG","year":"2020","unstructured":"Feysia SG, , Hasan-Nejad M, , Amini S, et al. Incidence, clinical manifestation, treatment outcome, and drug susceptibility pattern of nontuberculous mycobacteria in HIV patients in Tehran, Iran. Ethiop J Health Sci, 2020; 30(1):75\u201384.","journal-title":"Ethiop J Health Sci"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s44350-024-00001-2"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4902941"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1183\/23120541.00773-2023"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-025-03843-6"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2023.08.035"},{"key":"e_1_3_6_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11070873"},{"key":"e_1_3_6_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1554-7"},{"key":"e_1_3_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jctube.2024.100463"},{"key":"e_1_3_6_34_2","doi-asserted-by":"publisher","DOI":"10.4103\/1735-1995.187306"},{"key":"e_1_3_6_35_2","doi-asserted-by":"crossref","unstructured":"de Carvalho LP Subtil F Machado T et al. A macroevolution-inspired approach to reveal novel antibiotic resistance mechanisms. 2024.","DOI":"10.21203\/rs.3.rs-3838489\/v1"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10766294251375421","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10766294251375421","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10766294251375421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T01:20:33Z","timestamp":1777684833000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1177\/10766294251375421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1177\/10766294251375421"],"URL":"https:\/\/doi.org\/10.1177\/10766294251375421","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]}}}