{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:08:00Z","timestamp":1777932480387,"version":"3.51.4"},"reference-count":53,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP243fa627005"],"award-info":[{"award-number":["JP243fa627005"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP24wm0125008"],"award-info":[{"award-number":["JP24wm0125008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    contamination in chicken meat, particularly with fluoroquinolone- and macrolide-resistant strains, is a significant public health concern. Therefore, developing an easy and rapid detection method for fluoroquinolone- and macrolide-resistant\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    will improve food safety. In this study, we designed and evaluated a multiplex-Polymerase Chain Reaction (PCR) method for the simultaneous detection of fluoroquinolone- and macrolide-resistant\n                    <jats:italic toggle=\"yes\">Campylobacter jejuni<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Campylobacter coli<\/jats:italic>\n                    in chicken meat. Our multiplex-PCR-targeted mutations in the\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">rrl<\/jats:italic>\n                    genes of\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    species confer resistance to fluoroquinolones and macrolides, respectively. Overall, this method could detect\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    contamination and identify\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">C. coli<\/jats:italic>\n                    as well as their susceptibility to fluoroquinolone and macrolide. Spiking of chicken meat samples revealed that multiplex-PCR can detect at least 0.72 Colony-Forming Unit (CFU)\/g\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    \/\n                    <jats:italic toggle=\"yes\">coli<\/jats:italic>\n                    . Among 277 retail chicken meat samples,\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    \/\n                    <jats:italic toggle=\"yes\">coli<\/jats:italic>\n                    were detected in 54 samples using multiplex-PCR, with isolation from 52 samples using microbial culture. The susceptibility of the isolates to fluoroquinolones and macrolides was determined using both the PCR and culture methods, and a comparison of these methods showed that the accuracy, specificity, and sensitivity were all above 95%. The culture method required approximately 8 days for\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    isolation and antimicrobial susceptibility testing. Multiplex-PCR could determine the contamination with\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    \/\n                    <jats:italic toggle=\"yes\">coli<\/jats:italic>\n                    and susceptibility to fluoroquinolones and macrolides within 2\u20133 days from the enrichment culture to final determination. These results indicate that the proposed multiplex-PCR enables rapid and simultaneous detection of fluoroquinolone- and macrolide-resistant\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    \/\n                    <jats:italic toggle=\"yes\">coli<\/jats:italic>\n                    in chicken meat and its utility for monitoring their contamination in food.\n                  <\/jats:p>","DOI":"10.1177\/10766294251389592","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:36:29Z","timestamp":1761006989000},"page":"1-8","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous Detection of Fluoroquinolone- and Macrolide-Resistant\n                    <i>Campylobacter jejuni\/Campylobacter coli<\/i>\n                    in Retail Chicken Meat Using Multiplex-PCR"],"prefix":"10.1177","volume":"32","author":[{"given":"Akira","family":"Fukuda","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayu","family":"Matsui","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuma","family":"Furuya","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo, Japan."},{"name":"International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Hokkaido, Japan."},{"name":"Division of Research Support, Hokkaido University Institute for Vaccine Research and Development, Sapporo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Tamura","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chie","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo, Japan."},{"name":"International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Hokkaido, Japan."},{"name":"Vaccinology for Clinical Development, Hokkaido University Institute for Vaccine Research and Development, Sapporo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Usui","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-15"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS13090904"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.11.019"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/NRGASTRO.2011.191"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01668-24"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.2753-2759.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00161-08"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/FPD.2018.2554"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2021.604933"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.5740\/JAOACINT.13-060"},{"key":"e_1_3_3_12_2","volume-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals","author":"CLSI","year":"2024","unstructured":"CLSI. 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