{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T16:10:36Z","timestamp":1779120636960,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:00:00Z","timestamp":1774656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"\n Background:<\/jats:title>\n \n Carbapenem-resistant\n Pseudomonas aeruginosa<\/jats:italic>\n (CRPA) and multidrug-resistant (MDR)\n P. aeruginosa<\/jats:italic>\n limit therapeutic options but have been sparsely documented in Ghana.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n From November 2023 to December 2024, we conducted a prospective cross-sectional study of\n P. aeruginosa<\/jats:italic>\n isolates from acute-care hospitals in Greater Accra, Ghana. Isolates were identified by matrix-assisted laser desorption ionization\u2013time of flight, and antimicrobial susceptibility was assessed by disk diffusion as per Clinical Laboratory Standard Institute guidelines. Meropenem-resistant isolates with positive carbapenemase phenotype were subjected to whole-genome sequencing. Multivariable logistic regression models identified risk factors for infections caused by MDR and\n carbapenemase-producing Pseudomonas aeruginosa<\/jats:italic>\n (CRPA).\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n P. aeruginosa<\/jats:italic>\n accounted for 0.32% (\n n<\/jats:italic>\n = 267\/83,589) of all bacterial infections identified from submitted clinical specimens and 2.82% (\n n<\/jats:italic>\n = 267\/12,236) of culture-positive infections. Of the 267\n P. aeruginosa<\/jats:italic>\n isolates, 20.2% (\n n<\/jats:italic>\n = 54\/267) were MDR and 13.5% (\n n<\/jats:italic>\n = 36\/267) were CRPA. Amikacin retained the highest activity against\n P. aeruginosa<\/jats:italic>\n . The mean multiple antibiotic resistance index among MDR isolates (0.51 \u00b1 0.26) was significantly higher than that among non-MDR\n P.aeruginosa<\/jats:italic>\n isolates\u00a0(0.02 \u00b1 0.07;\n p<\/jats:italic>\n < 0.001), with a large between-group difference (Hedges\u2019\n g<\/jats:italic>\n = 3.70). Only one isolate (2.7%) harbored a single carbapenemase gene,\n blaNDM-1<\/jats:italic>\n . The remaining 35 carried a\n blaOXA-50<\/jats:italic>\n -type backbone that co-occurred with either class A carbapenemases (\n blaKPC<\/jats:italic>\n [\n n<\/jats:italic>\n = 7],\n blaSME-1<\/jats:italic>\n [\n n<\/jats:italic>\n = 4],\n blaGES-5<\/jats:italic>\n [\n n<\/jats:italic>\n = 1]) or class B metallo-\u03b2-lactamases (\n blaNDM-1<\/jats:italic>\n [\n n<\/jats:italic>\n = 19],\n blaVIM-5<\/jats:italic>\n [\n n<\/jats:italic>\n = 3],\n blaIMP-15<\/jats:italic>\n [\n n<\/jats:italic>\n = 1]).\n blaNDM-1<\/jats:italic>\n was the most dominant carbapenemase gene (\n n<\/jats:italic>\n = 20\/36) . Wound infection was the strongest predictor of MDR infections (adjusted odds ratio [aOR] = 3.01; 95% confidence interval (CI) = 1.43\u20134.47;\n p<\/jats:italic>\n = 0.001], whereas inpatient status was the strongest predictor of CRPA infection (aOR = 3.32; 95% CI = 0.98\u20134.09;\n p<\/jats:italic>\n = 0.001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n MDR\n P. aeruginosa<\/jats:italic>\n and carbapenem Resistant\n P.aeruginosa<\/jats:italic>\n (CRPA), mostly\n blaNDM-1<\/jats:italic>\n producers, are major causes of infection in our setting. Restricting carbapenem use through stewardship and strengthening infection control is essential to limit CRPA spread.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/10766294261436119","type":"journal-article","created":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T16:07:24Z","timestamp":1774714044000},"page":"215-225","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["A Multicenter Surveillance of Antimicrobial Resistance Among\n Pseudomonas aeruginosa<\/i>\n in Hospitals of the Greater Accra Region of Ghana"],"prefix":"10.1177","volume":"32","author":[{"given":"Francisca","family":"Duah","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4922-4916","authenticated-orcid":false,"given":"Noah","family":"Obeng-Nkrumah","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, School of Biomedical and Allied Health Sciences, University of Ghana, Legon, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beverly","family":"Egyir","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute of Medical Research, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Japheth","family":"Opintan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amoako","family":"Duah","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Ghana Medical Center, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Boateng","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary-Magdalene","family":"Osei","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Baffuor-Asare","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Sampane-Donkor","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Accra, Ghana."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,3,28]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1159798"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.4314\/ejhs.v33i3.5"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-024-03640-7"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-023-08230-y"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2048972"},{"key":"e_1_3_5_7_1","unstructured":"6.The World Health Organization. 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