{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T18:05:38Z","timestamp":1789236338346,"version":"build-2803163510"},"reference-count":38,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Pseudomonas asiaticais<\/jats:italic>\n                    an emerging opportunistic pathogen with a broad host range. Current evidence suggests that some isolates exhibit multidrug resistance, which may complicate treatment. In this study, a multidrug-resistant\n                    <jats:italic toggle=\"yes\">P. asiatica<\/jats:italic>\n                    strain MD9 was isolated from aquaculture water. We aimed to characterize its complete genome sequence and investigate the role of its conjugative megaplasmid pMD9A in the horizontal transfer of antibiotic resistance genes. The genome of MD9 consists of one circular chromosome (5,956,782 bp, with a G + C content of 62.5%) and one circular megaplasmid, pMD9A (455,169 bp, with a G + C content of 56.5%). Genome annotation identified 65 antibiotic resistance genes and 148 putative virulence factor-encoding genes in the MD9 genome. The megaplasmid pMD9A carries 29 antibiotic resistance genes conferring resistance to \u03b2-lactams, chloramphenicol\/florfenicol, aminoglycosides, and macrolides. A class 1 integron (\n                    <jats:italic toggle=\"yes\">intI<\/jats:italic>\n                    1) and multiple autonomous conjugative transfer elements were identified in pMD9A. Conjugation experiments demonstrated that the \u03b2-lactam resistance gene\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-246<\/jats:sub>\n                    could be horizontally transferred from the donor MD9 strain to the recipient\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    25DN strain. The megaplasmid pMD9A not only carries a broad array of antibiotic resistance genes, but also facilitates their horizontal spread among environmental bacteria, thereby potentially contributing to the dissemination of multidrug-resistant bacteria.\n                  <\/jats:p>","DOI":"10.1177\/10766294261484663","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:49:04Z","timestamp":1788947344000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["The Conjugative Megaplasmid pMD9A Mediates Transferring Antibiotic Resistance Genes"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1327-515X","authenticated-orcid":false,"given":"Yahan","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Aquaculture, Yantai Institute of China Agricultural University, Yantai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-jia","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Public Health, Yale University, New Haven, Connecticut, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TianQi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Yantai Institute of China Agricultural University, Yantai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Yantai Institute of China Agricultural University, Yantai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.24171\/j.phrp.2023.0181"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2026.126007"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12327"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-024-01824-x"},{"issue":"4","key":"e_1_3_3_6_1","first-page":"1432","article-title":"Antimicrobial resistance apocalypse and pan-drug-resistant superbug challenges: Current status, impact and mitigation strategies","volume":"45","author":"Hossain H","year":"2025","unstructured":"5.Hossain H, , Cm Rahman MN, , Khan SS, et al. Antimicrobial resistance apocalypse and pan-drug-resistant superbug challenges: Current status, impact and mitigation strategies. Pak Vet J 2025;45(4):1432\u20131447; doi: 10.29261\/pakvetj\/2025.254","journal-title":"Pak Vet J"},{"issue":"2","key":"e_1_3_3_7_1","first-page":"499","article-title":"Antibiotic resistance and emerging alternatives for controlling foodborne pathogens","volume":"45","author":"Alsayeqh AF","year":"2025","unstructured":"6.Alsayeqh AF. Antibiotic resistance and emerging alternatives for controlling foodborne pathogens. Pak Vet J 2025;45(2):499\u2013514; doi: 10.29261\/pakvetj\/2025.185","journal-title":"Pak Vet J"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envc.2025.101401"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13152448"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fsufs.2026.1801220"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00232-25"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.16079"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2025.05.022"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00907-24"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12060574"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2025.09.012"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003316"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001258"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-10-25-2087-PDN"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00475-19"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/15320383.2022.2159321"},{"key":"e_1_3_3_22_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","author":"CLSI","year":"2025","unstructured":"21.CLSI. 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Exploring the diversity and dissemination dynamics of antimicrobial resistance genes in enterobacteriaceae plasmids across varied ecological niches. Pakistan Veterinary J 2025;45(2):662\u2013672; doi: 10.29261\/pakvetj\/2025.190","journal-title":"Pakistan Veterinary J"},{"issue":"3","key":"e_1_3_3_32_1","first-page":"812","article-title":"Molecular characterization and drug resistance pattern of pseudomonas aeruginosa isolated from poultry meat and meat products","volume":"44","author":"Abdullah RM","year":"2024","unstructured":"31.Abdullah RM, , Ali S, , Aslam B, et al. Molecular characterization and drug resistance pattern of pseudomonas aeruginosa isolated from poultry meat and meat products. 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