{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T12:04:55Z","timestamp":1780574695186,"version":"3.54.1"},"reference-count":36,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Interferon &amp; Cytokine Research"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>\n                    Osteoarthritis is a degenerative joint disease characterized by synovial inflammation and cartilage destruction. This study aimed to investigate the function of\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    in regulating human fibroblast-like synoviocytes (HFLS) and its subsequent impact on chondrocyte degeneration. Differential expression and pathway analyses were performed according to the Gene Expression Omnibus (GEO) dataset. HFLS were stimulated with IL-1\u03b2, and\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    was knocked down via transfection. Inflammatory signaling, cytokine expression, and extracellular vesicles (EVs) secretion were assessed. Isolated EVs were characterized by nanoparticle tracking analysis, transmission electron microscopy, and Western blotting. The functional effects of HFLS-derived EVs on chondrocyte inflammation, extracellular matrix metabolism, apoptosis, and viability were evaluated. Bioinformatics analysis identified\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    as a key gene linked to the endocytosis pathway. In IL-1\u03b2-stimulated HFLS,\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    expression was upregulated.\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    knockdown reduced the activation of p38 and NF-\u03baB pathways and suppressed pro-inflammatory factors and matrix degradation mediators. Furthermore,\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    deficiency impaired EV secretion and altered their cargo. Crucially, while EVs from control HFLS promoted chondrocyte catabolism, inflammation, and apoptosis, EVs from\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    -knockdown HFLS mitigated these degenerative phenotypes. Our findings demonstrate that\n                    <jats:italic toggle=\"yes\">RAB5A<\/jats:italic>\n                    , by modulating the biogenesis and composition of HFLS-derived EVs, plays a critical role in driving chondrocyte degeneration, highlighting its importance as a key molecular regulator in osteoarthritis.\n                  <\/jats:p>","DOI":"10.1177\/10799907261435767","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T15:40:18Z","timestamp":1775662818000},"page":"171-180","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>RAB5A<\/i>\n                    Governs Synovial Fibroblast Extracellular Vesicles and Mediates Chondrocyte Degeneration in Osteoarthritis"],"prefix":"10.1177","volume":"46","author":[{"given":"Zhenxing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics, Haining People\u2019s Hospital, Jiaxing City, Zhejiang Province, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangchun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Haining Maternal and Child Health Hospital, Jiaxing City, Zhejiang Province, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiongfei","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Haining People\u2019s Hospital, Jiaxing City, Zhejiang Province, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Haining People\u2019s Hospital, Jiaxing City, Zhejiang Province, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan\u2019ang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Haining People\u2019s Hospital, Jiaxing City, Zhejiang Province, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0433-8220","authenticated-orcid":false,"given":"Zhen","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Haining People\u2019s Hospital, Jiaxing City, Zhejiang Province, P.R. 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