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This study evaluates their biochemical interaction, along with their connection to the nuclear factor kappa beta (NF-\u03ba\u03b2)\/transforming growth factor-beta (TGF-\u03b2) signaling pathway and crosstalk with vascular endothelial growth factor (VEGF) and apoptotic markers in CRC. Serum samples were collected from 120 participants, including 90 CRC patients exhibiting a range of tumor grades and anatomical sites, in addition to 30 healthy controls. The expression levels of NF-\u03baB and apoptotic markers were quantified using standardized immunoassays, while the relative expression levels of MALAT1, miR-145, TGF-\u03b2, and VEGF were assessed using real-time polymerase chain reaction. Integrative analysis of CRC samples reveals that MALAT1 overexpression correlates with downregulation of miR-145, increased NF-\u03ba\u03b2, elevated levels of TGF-\u03b2 and VEGF, and alterations in apoptotic proteins. Moreover, receiver operating characteristic analysis revealed excellent discriminative power for MALAT1 and miR-145 in distinguishing patients with CRC from healthy controls. These findings suggest that the MALAT1\/miR-145 axis serves as a central biochemical node linking inflammatory and fibrotic signaling pathways and apoptotic protein expression in colorectal carcinogenesis. Targeting this regulatory network may offer novel therapeutic opportunities to suppress NF-\u03ba\u03b2\/TGF-\u03b2-driven tumor progression and improve CRC outcomes.<\/jats:p>","DOI":"10.1177\/10799907261444320","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T10:34:38Z","timestamp":1776767678000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Biochemical Signature in Deciphering the Metastasis-Associated Lung Adenocarcinoma Transcript 1\/microRNA-145 Axis and Its Molecular Correlation with Nuclear Factor Kappa Beta\/Transforming Growth Factor-Beta and Apoptotic Proteins Expression in Colorectal Cancer"],"prefix":"10.1177","author":[{"given":"Asmaa","family":"Atef","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Beni-Suef University, Beni Suef, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basant","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Beni-Suef University, Beni Suef, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basma","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Beni-Suef University, Beni Suef, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed S.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Oncology, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1034-0458","authenticated-orcid":false,"given":"Nabil A.","family":"Hasona","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Beni-Suef University, Beni Suef, Egypt."},{"name":"Faculty of Science, Biochemistry-Microbiology Program, Beni Suef National University, Beni Suef, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/jbaar.2022.270833"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12291-022-01101-4"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/mco.2021.2413"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26052191"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2025.14863"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrna.2023.01.001"},{"issue":"6","key":"e_1_3_2_8_1","first-page":"3865","article-title":"VEGF-1 expression in colorectal cancer is associated with disease localization, stage, and long-term disease-specific survival","volume":"28","author":"Bendardaf R","year":"2008","unstructured":"Bendardaf R, , Buhmeida A, , Hilska M, et al. 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