{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:04:29Z","timestamp":1789653869597,"version":"4.0.0"},"reference-count":39,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Interferon &amp; Cytokine Research"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>\n                    As a key component of the replication protein A (RPA) complex,\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    has been identified as oncogenic in multiple solid tumors. However, its specific role in breast cancer remains poorly understood.\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    expression and its prognostic relevance in breast cancer were assessed based on the public databases. To further confirm the biological function of\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    , we knocked down\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    in the breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and then conducted Cell Counting Kit-8, colony formation, Western blot, immunofluorescence, and transmission electron microscopy.\n                    <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                    effects of\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    were tested in a xenograft model. We found that high expression of\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    in breast cancer predicted adverse patient outcomes.\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    was mainly involved in multiple oncogenic signaling pathways, including the transforming growth factor-\u03b2 (TGF-\u03b2) pathway.\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    knockdown effectively suppressed cancer cell proliferation\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                    . Mechanistically,\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    knockdown decreased TGF-\u03b21 promoter activity and reduced TGF-\u03b21 expression at mRNA and protein levels, accompanied by decreased p-Smad2\/3 levels.\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    knockdown also significantly blocked autophagy, as evidenced by decreased microtubule-associated protein 1 light chain 3 (LC3)-II\/LC3-I ratio, increased sequestosome 1 (p62) level, and reduced LC3 puncta. Notably, pharmacological activation of the TGF-\u03b2 pathway partially reversed autophagy alterations induced by\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    knockdown. These data support the possibility of\n                    <jats:italic toggle=\"yes\">RPA3<\/jats:italic>\n                    as a therapeutic target for breast cancer.\n                  <\/jats:p>","DOI":"10.1177\/10799907261465503","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T11:31:38Z","timestamp":1783337498000},"page":"285-293","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted Inhibition of\n                    <i>RPA3<\/i>\n                    Impairs Breast Cancer Progression Through Suppressing TGF-\u03b2 Signaling Pathway-Mediated Autophagy"],"prefix":"10.1177","volume":"46","author":[{"given":"Zhiyi","family":"Zhang","sequence":"first","affiliation":[{"name":"Huguang Internal Medicine Ward \u2161, Jilin Cancer Hospital, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Internal Medicine Ward IV, Changling County People\u2019s Hospital, Songyuan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5759-3281","authenticated-orcid":false,"given":"Liqun","family":"Hao","sequence":"additional","affiliation":[{"name":"Huguang Internal Medicine Ward \u2161, Jilin Cancer Hospital, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41523-023-00524-3"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-022-02476-1"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13060960"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2020.1813070"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26157326"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.1874209"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21978"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-06944-8"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04574-w"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.Cd-21-1059"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058-025-02101-x"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-025-02667-8"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.119000"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.34618"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1797280"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-024-00653-2"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-1138-4"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2023.69.5.14"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05972-0"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06154-8"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmw041"},{"key":"e_1_3_2_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2025.05.004"},{"key":"e_1_3_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2020.103032"},{"key":"e_1_3_2_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom14101219"},{"key":"e_1_3_2_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25010588"},{"key":"e_1_3_2_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061447"},{"key":"e_1_3_2_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-022-00684-0"},{"key":"e_1_3_2_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13200"},{"key":"e_1_3_2_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2024.107534"},{"key":"e_1_3_2_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom14080922"},{"key":"e_1_3_2_33_1","doi-asserted-by":"publisher","DOI":"10.4103\/jcrt.jcrt_275_21"},{"key":"e_1_3_2_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.826655"},{"key":"e_1_3_2_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.865067"},{"key":"e_1_3_2_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12081156"},{"key":"e_1_3_2_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-02108-4"},{"key":"e_1_3_2_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2009.11.014"},{"key":"e_1_3_2_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-022-04672-w"},{"key":"e_1_3_2_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2021.07.010"}],"container-title":["Journal of Interferon &amp; 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