{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:22:35Z","timestamp":1777724555067,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Investigative Medicine"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>\n                    Atherosclerosis, a major cause of cardiovascular diseases, is characterized by the accumulation of oxidized lipoproteins (ox-LDL) within arterial walls, leading to inflammation and plaque formation. Hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) has demonstrated anti-inflammatory and vascular protective properties, but its role in modulating macrophage endocytosis of ox-LDL and its impact on early atherosclerosis development remains unclear. Macrophage cultures were utilized for ox-LDL uptake experiments. Macrophages were pretreated with sodium hydrosulfide (NaHS) (50\u2009\u03bcmol\/L) or propargylglycine (PPG, 3\u2009mmol\/L) for 1\u2009h, followed by incubation with DiI-ox-LDL (10\u2009\u03bcg\/mL) for an additional 2\u2009h. DiI-ox-LDL uptake was visualized using live-cell imaging. The expression of scavenger receptors CD36 and SR-A was assessed through immunofluorescent staining and western blot analysis. To determine the intracellular signal transduction pathways involved, macrophages were pretreated with NF-\u03baB pathway blocker pyrrolidine dithiocarbamate or MAPK inhibitor PD98059 before the addition of NaHS. NaHS significantly inhibited ox-LDL uptake by macrophages, while PPG treatment markedly increased this process. Immunocytochemistry and western blot analysis revealed that the expressions of CD36 and SR-A were induced by ox-LDL but inhibited by NaHS in a concentration- and time-dependent manner. Furthermore, H\n                    <jats:sub>2<\/jats:sub>\n                    S down-regulated ox-LDL receptors CD36 and SR-A through the NF-\u03baB signal pathway. H\n                    <jats:sub>2<\/jats:sub>\n                    S inhibits early atherosclerosis development by modulating macrophage uptake of ox-LDL through the down-regulation of CD36 and SR-A receptors via the NF-\u03baB signaling pathway. These findings provide new evidence for the role of H\n                    <jats:sub>2<\/jats:sub>\n                    S in atherosclerosis and its potential therapeutic value.\n                  <\/jats:p>","DOI":"10.1177\/10815589241279599","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:59:51Z","timestamp":1724893191000},"page":"947-955","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Hydrogen sulfide inhibits early development of atherosclerosis by modulating macrophage uptake of oxidized lipoproteins"],"prefix":"10.1177","volume":"72","author":[{"given":"Nan","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Neurology, Shaoxing Central Hospital, Shaoxing, China"},{"name":"The Central Affiliated Hospital, Shaoxing University, Shaoxing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Yang","sequence":"additional","affiliation":[{"name":"Lab Center, Medical College of Soochow University, Suzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanmei","family":"Liu","sequence":"additional","affiliation":[{"name":"Lab Center, Medical College of Soochow University, Suzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiyun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical College, Soochow University, Suzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901143400207"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2020.11.010"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03392-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-03-01066.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2013.07.002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5324"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.036854"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.179333"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2015.5"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.140627"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2010.010308"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030584"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20211314"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1251499"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-1153-2"},{"key":"e_1_3_2_17_2","first-page":"601","article-title":"Geniposide protects against ox-LDL-induced foam cell formation through inhibition of MAPKs and NF-kB signaling pathways","volume":"74","author":"Li Z-Q","year":"2019","unstructured":"Li Z-Q, Huang X-Y, Hu C-Y, Zhu Z-S, Chen Y, Gong M. Geniposide protects against ox-LDL-induced foam cell formation through inhibition of MAPKs and NF-kB signaling pathways. Pharmazie. 2019; 74: 601\u2013605.","journal-title":"Pharmazie"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2022.0075"},{"key":"e_1_3_2_19_2","first-page":"915983","article-title":"Chronic NaHS treatment is vasoprotective in high-fat-fed ApoE(-\/-) mice","volume":"2013","author":"Ford A","year":"2013","unstructured":"Ford A, Al-Magableh M, Gaspari TA, Hart JL. Chronic NaHS treatment is vasoprotective in high-fat-fed ApoE(-\/-) mice. Int J Vasc Med. 2013; 2013: 915983.","journal-title":"Int J Vasc Med"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2019.00049"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3721"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.06.005"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00204.2016"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/21623945.2022.2123097"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S431026"}],"container-title":["Journal of Investigative Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10815589241279599","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10815589241279599","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10815589241279599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:40:13Z","timestamp":1777459213000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10815589241279599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,26]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1177\/10815589241279599"],"URL":"https:\/\/doi.org\/10.1177\/10815589241279599","relation":{},"ISSN":["1081-5589","1708-8267"],"issn-type":[{"value":"1081-5589","type":"print"},{"value":"1708-8267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,26]]}}}