{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:51:18Z","timestamp":1792457478700,"version":"4.5.0"},"reference-count":27,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":5685,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2006,10]]},"DOI":"10.1177\/1087057106291585","type":"journal-article","created":{"date-parts":[[2006,8,23]],"date-time":"2006-08-23T20:59:45Z","timestamp":1156366785000},"page":"836-843","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["A Rapid Transglutaminase Assay for High-Throughput Screening Applications"],"prefix":"10.1016","volume":"11","author":[{"given":"Yu-Wei","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hui","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1177\/1087057106291585_bib1","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1146\/annurev.bi.49.070180.002505","article-title":"Transglutaminases.","volume":"49","author":"Folk","year":"1980","journal-title":"Annu Rev Biochem"},{"key":"10.1177\/1087057106291585_bib2","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1111\/j.1742-4658.2004.04476.x","article-title":"Mammalian transglutaminases: identification of substrates as a key to physiological function and physiopathological relevance.","volume":"272","author":"Esposito","year":"2005","journal-title":"FEBS J"},{"key":"10.1177\/1087057106291585_bib3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S1357-2725(03)00239-5","article-title":"The pathogenesis of coeliac disease.","volume":"36","author":"Dewar","year":"2004","journal-title":"Int J Biochem Cell Biol"},{"key":"10.1177\/1087057106291585_bib4","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s004390050273","article-title":"Transglutaminase activity is related to CAG repeat length in patients with Huntington\u2019s disease.","volume":"98","author":"Cariello","year":"1996","journal-title":"Hum Genet"},{"key":"10.1177\/1087057106291585_bib5","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s00253-003-1539-5","article-title":"Properties and applications of microbial transglutaminase.","volume":"64","author":"Yokoyama","year":"2004","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1177\/1087057106291585_bib6","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/0378-4347(95)00562-5","article-title":"Transglutaminases: purification and activity assays.","volume":"684","author":"Wilhelm","year":"1996","journal-title":"J Chromatogr B Biomed Appl"},{"key":"10.1177\/1087057106291585_bib7","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0003-2697(71)90362-9","article-title":"Transamidating enzymes: I. Rapid chromatographic assays.","volume":"44","author":"Lorand","year":"1971","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib8","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/0003-2697(71)90363-0","article-title":"Transamidating enzymes: II. A continuous fluorescent method suited for automating measurements of factor XIII in plasma.","volume":"44","author":"Lorand","year":"1971","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib9","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0003-2697(86)90095-3","article-title":"Modification of transglutaminase assay: use of ammonium sulfate to stop the reaction.","volume":"153","author":"Takagi","year":"1986","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib10","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1006\/abio.1997.2139","article-title":"A fluorescent substrate of transglutaminase for detection and characterization of glutamine acceptor compounds.","volume":"249","author":"Pasternack","year":"1997","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib11","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1006\/abio.2001.5040","article-title":"A sensitive fluorometric assay for tissue transglutaminase.","volume":"292","author":"Jeitner","year":"2001","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib12","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/0003-2697(72)90074-7","article-title":"A filter paper assay for transamidating enzymes using radioactive amine substrates.","volume":"50","author":"Lorand","year":"1972","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib13","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.ab.2005.05.034","article-title":"High-throughput scintillation proximity assay for transglutaminase activity measurement.","volume":"343","author":"Madi","year":"2005","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib14","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1006\/abio.1994.1551","article-title":"A microtiter assay for factor XIII using fibrinogen and biotinylcadaverine as substrates.","volume":"223","author":"Song","year":"1994","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib15","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.ab.2004.03.068","article-title":"An improved colorimetric assay for the measurement of transglutaminase (type II)\u2013(gamma-glutamyl) lysine cross-linking activity.","volume":"330","author":"Trigwell","year":"2004","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib16","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0926-6593(66)90066-X","article-title":"Transglutaminase: mechanistic features of the active site as determined by kinetic and inhibitor studies.","volume":"122","author":"Folk","year":"1966","journal-title":"Biochim Biophys Acta"},{"key":"10.1177\/1087057106291585_bib17","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1006\/abio.2000.4713","article-title":"A direct continuous spectrophotometric assay for transglutaminase activity.","volume":"285","author":"de Macedo","year":"2000","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib18","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1093\/clinchem\/46.12.1946","article-title":"A modified, optimized kinetic photometric assay for the determination of blood coagulation factor XIII activity in plasma.","volume":"46","author":"Karpati","year":"2000","journal-title":"Clin Chem"},{"key":"10.1177\/1087057106291585_bib19","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1006\/exer.1997.0463","article-title":"Novel inhibitors against the transglutaminase-catalysed crosslinking of lens proteins.","volume":"66","author":"Lorand","year":"1998","journal-title":"Exp Eye Res"},{"key":"10.1177\/1087057106291585_bib20","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.ab.2004.09.047","article-title":"Development of a mechanism-based assay for tissue transglutaminase: results of a high-throughput screen and discovery of inhibitors.","volume":"338","author":"Case","year":"2005","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib21","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.ab.2005.09.035","article-title":"A direct fluorometric assay for tissue transglutaminase.","volume":"347","author":"Gillet","year":"2005","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib22","series-title":"Physical Biochemistry: Applications to Biochemistry and Molecular Biology.","first-page":"323","article-title":"Immunological methods","author":"Freifelder","year":"1982"},{"key":"10.1177\/1087057106291585_bib23","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0003-2697(81)90534-0","article-title":"Dansylation of amino acids for high-performance liquid chromatography analysis.","volume":"115","author":"Tapuhi","year":"1981","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib24","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0165-022X(00)00136-6","article-title":"Dansylation of hydroxyl and carboxylic acid functional groups.","volume":"47","author":"Bartzatt","year":"2001","journal-title":"J Biochem Biophys Methods"},{"key":"10.1177\/1087057106291585_bib25","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1177\/1087057106291585_bib26","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.biocel.2004.07.006","article-title":"Characterization of purified rat testicular transglutaminase and age-dependent changes of the enzyme activities.","volume":"37","author":"Wu","year":"2005","journal-title":"Int J Biochem Cell Biol"},{"key":"10.1177\/1087057106291585_bib27","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.bcp.2004.12.011","article-title":"Mechanism for the inhibition of transglutaminase 2 by cystamine.","volume":"69","author":"Jeitner","year":"2005","journal-title":"Biochem Pharmacol"}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1087057106291585","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555222085252?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555222085252?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1087057106291585","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T11:31:55Z","timestamp":1762860715000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2472555222085252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["S2472555222085252"],"URL":"https:\/\/doi.org\/10.1177\/1087057106291585","relation":{},"ISSN":["2472-5552"],"issn-type":[{"value":"2472-5552","type":"print"}],"subject":[],"published":{"date-parts":[[2006,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Rapid Transglutaminase Assay for High-Throughput Screening Applications","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1177\/1087057106291585","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2006 Society for Laboratory Automation and Screening. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}