{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:52:18Z","timestamp":1778885538370,"version":"3.51.4"},"reference-count":27,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":4954,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2008,10]]},"DOI":"10.1177\/1087057108323909","type":"journal-article","created":{"date-parts":[[2008,9,23]],"date-time":"2008-09-23T21:40:58Z","timestamp":1222206058000},"page":"870-878","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Identification of Small Molecule Inhibitors of \u03b2-Amyloid Cytotoxicity through a Cell-Based High-Throughput Screening Platform"],"prefix":"10.1016","volume":"13","author":[{"given":"K.I.","family":"Seyb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.R.","family":"Schuman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.M.","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.L.","family":"Michaelis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"Glicksman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1177\/1087057108323909_bib1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1126\/science.2474201","article-title":"Neurotoxicity of a fragment of the amyloid precursor associated with Alzheimer\u2019s disease.","volume":"245","author":"Yankner","year":"1989","journal-title":"Science"},{"key":"10.1177\/1087057108323909_bib2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0006-8993(91)91553-D","article-title":"In vitro aging of beta-amyloid protein causes peptide aggregation and neurotoxicity.","volume":"563","author":"Pike","year":"1991","journal-title":"Brain Res"},{"key":"10.1177\/1087057108323909_bib3","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1016\/S0896-6273(00)80115-4","article-title":"Mechanisms of neuronal degeneration in Alzheimer\u2019s disease.","volume":"16","author":"Yankner","year":"1996","journal-title":"Neuron"},{"key":"10.1177\/1087057108323909_bib4","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.bbadis.2004.07.002","article-title":"Inability of tau to properly regulate neuronal microtubule dynamics: a loss-of-function mechanism by which tau might mediate neuronal cell death.","volume":"1739","author":"Feinstein","year":"2005","journal-title":"Biochim Biophys Acta"},{"key":"10.1177\/1087057108323909_bib5","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0014-5793(99)01279-X","article-title":"Inhibition of tau phosphorylating protein kinase cdk5 prevents beta-amyloid-induced neuronal death.","volume":"459","author":"Alvarez","year":"1999","journal-title":"FEBS Lett"},{"key":"10.1177\/1087057108323909_bib6","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1046\/j.1471-4159.2003.01526.x","article-title":"Stabilization of the cyclin-dependent kinase 5 activator, p35, by paclitaxel decreases beta-amyloid toxicity in cortical neurons.","volume":"84","author":"Li","year":"2003","journal-title":"J Neurochem"},{"key":"10.1177\/1087057108323909_bib7","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0168-0102(99)00026-7","article-title":"Elevated neuronal Cdc2-like kinase activity in the Alzheimer disease brain.","volume":"34","author":"Lee","year":"1999","journal-title":"Neurosci Res"},{"key":"10.1177\/1087057108323909_bib8","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/45159","article-title":"Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration.","volume":"402","author":"Patrick","year":"1999","journal-title":"Nature"},{"key":"10.1177\/1087057108323909_bib9","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/371419a0","article-title":"p35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5.","volume":"371","author":"Tsai","year":"1994","journal-title":"Nature"},{"issue":"pt 6","key":"10.1177\/1087057108323909_bib10","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1242\/jcs.113.6.975","article-title":"p39 activates cdk5 in neurons, and is associated with the actin cytoskeleton.","volume":"113","author":"Humbert","year":"2000","journal-title":"J Cell Sci"},{"key":"10.1177\/1087057108323909_bib11","doi-asserted-by":"crossref","first-page":"17166","DOI":"10.1074\/jbc.M907757199","article-title":"Calpain-dependent proteolytic cleavage of the p35 cyclin-dependent kinase 5 activator to p25.","volume":"275","author":"Kusakawa","year":"2000","journal-title":"J Biol Chem"},{"key":"10.1177\/1087057108323909_bib12","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/35012636","article-title":"Neurotoxicity induces cleavage of p35 to p25 by calpain.","volume":"405","author":"Lee","year":"2000","journal-title":"Nature"},{"key":"10.1177\/1087057108323909_bib13","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1523\/JNEUROSCI.12-02-00376.1992","article-title":"beta-Amyloid peptides destabilize calcium homeostasis and render human cortical neurons vulnerable to excitotoxicity.","volume":"12","author":"Mattson","year":"1992","journal-title":"J Neurosci"},{"key":"10.1177\/1087057108323909_bib14","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0006-8993(02)02549-0","article-title":"Evaluation of beta-amyloid peptide 25-35 on calcium homeostasis in cultured rat dorsal root ganglion neurons.","volume":"939","author":"He","year":"2002","journal-title":"Brain Res"},{"key":"10.1177\/1087057108323909_bib15","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1073\/pnas.90.7.2628","article-title":"Widespread activation of calcium-activated neutral proteinase (calpain) in the brain in Alzheimer disease: a potential molecular basis for neuronal degeneration.","volume":"90","author":"Saito","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1177\/1087057108323909_bib16","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/S0014-5793(00)02431-5","article-title":"Calpain-mediated degradation of p35 to p25 in postmortem human and rat brains.","volume":"489","author":"Taniguchi","year":"2001","journal-title":"FEBS Lett"},{"key":"10.1177\/1087057108323909_bib17","doi-asserted-by":"crossref","first-page":"2400","DOI":"10.1523\/JNEUROSCI.10-07-02400.1990","article-title":"Proteolytic processing of beta-amyloid precursor by calpain I.","volume":"10","author":"Siman","year":"1990","journal-title":"J Neurosci"},{"key":"10.1177\/1087057108323909_bib18","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1006\/bbrc.2000.2919","article-title":"Possible role of calpain in normal processing of beta-amyloid precursor protein in human platelets.","volume":"273","author":"Chen","year":"2000","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1177\/1087057108323909_bib19","doi-asserted-by":"crossref","first-page":"31746","DOI":"10.1074\/jbc.M503259200","article-title":"Beta-amyloid-induced dynamin 1 depletion in hippocampal neurons: a potential mechanism for early cognitive decline in Alzheimer disease.","volume":"280","author":"Kelly","year":"2005","journal-title":"J Biol Chem"},{"key":"10.1177\/1087057108323909_bib20","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1083\/jcb.150.4.887","article-title":"Cross-talk between two cysteine protease families: activation of caspase-12 by calpain in apoptosis.","volume":"150","author":"Nakagawa","year":"2000","journal-title":"J Cell Biol"},{"key":"10.1177\/1087057108323909_bib21","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1046\/j.1471-4159.2000.0741646.x","article-title":"Pretreatment with calpain inhibitor CEP-4143 inhibits calpain I activation and cytoskeletal degradation, improves neurological function, and enhances axonal survival after traumatic spinal cord injury.","volume":"74","author":"Schumacher","year":"2000","journal-title":"J Neurochem"},{"key":"10.1177\/1087057108323909_bib22","doi-asserted-by":"crossref","first-page":"4081","DOI":"10.1523\/JNEUROSCI.23-10-04081.2003","article-title":"Inhibition of calpains prevents neuronal and behavioral deficits in an MPTP mouse model of Parkinson\u2019s disease.","volume":"23","author":"Crocker","year":"2003","journal-title":"J Neurosci"},{"key":"10.1177\/1087057108323909_bib23","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1097\/01.WCB.0000138664.17682.32","article-title":"Caspase inhibitor z-DEVD-fmk attenuates calpain and necrotic cell death in vitro and after traumatic brain injury.","volume":"24","author":"Knoblach","year":"2004","journal-title":"J Cereb Blood Flow Metab"},{"key":"10.1177\/1087057108323909_bib24","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/0076-6879(94)44048-4","article-title":"Peptidyl diazomethanes as inhibitors of cysteine and serine proteinases.","volume":"244","author":"Shaw","year":"1994","journal-title":"Methods Enzymol"},{"key":"10.1177\/1087057108323909_bib25","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A simple statistical parameter for use in evaluation and validation of high throughput screening assays.","volume":"4","author":"Zhang","year":"1999","journal-title":"J Biomol Screen"},{"key":"10.1177\/1087057108323909_bib26","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1046\/j.1471-4159.2000.0750991.x","article-title":"Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor-dependent, human neuron-like cells.","volume":"75","author":"Encinas","year":"2000","journal-title":"J Neurochem"},{"key":"10.1177\/1087057108323909_bib27","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1006\/abio.2001.5346","article-title":"Retinoic acid evoked-differentiation of neuroblastoma cells predominates over growth factor stimulation: an automated image capture and quantitation approach to neuritogenesis.","volume":"298","author":"Simpson","year":"2001","journal-title":"Anal Biochem"}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1087057108323909","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555222082636?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555222082636?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1087057108323909","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T11:33:22Z","timestamp":1762860802000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2472555222082636"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":27,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["S2472555222082636"],"URL":"https:\/\/doi.org\/10.1177\/1087057108323909","relation":{},"ISSN":["2472-5552"],"issn-type":[{"value":"2472-5552","type":"print"}],"subject":[],"published":{"date-parts":[[2008,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of Small Molecule Inhibitors of \u03b2-Amyloid Cytotoxicity through a Cell-Based High-Throughput Screening Platform","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1177\/1087057108323909","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2008 Society for Laboratory Automation and Screening. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}