{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:11:43Z","timestamp":1787019103829,"version":"build-2736575974"},"reference-count":63,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Toxicol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:p>Earlier, we reported that mercury, the environmental risk factor for cardiovascular diseases, activates vascular endothelial cell (EC) phospholipase D (PLD). Here, we report the novel and significant finding that calcium and calmodulin regulated mercury-induced PLD activation in bovine pulmonary artery ECs (BPAECs). Mercury (mercury chloride, 25 \u03bcM; thimerosal, 25 \u03bcM; methylmercury, 10 \u03bcM) significantly activated PLD in BPAECs. Calcium chelating agents and calcium depletion of the medium completely attenuated the mercury-induced PLD activation in ECs. Calmodulin inhibitors significantly attenuated mercury-induced PLD activation in BPAECs. Despite the absence of L-type calcium channels in ECs, nifedipine, nimodipine, and diltiazem significantly attenuated mercury-induced PLD activation and cytotoxicity in BPAECs. This study demonstrated the importance of calcium and calmodulin in the regulation of mercury-induced PLD activation and the protective action of L-type calcium channel blockers against mercury cytotoxicity in vascular ECs, suggesting mechanisms of mercury vasculotoxicity and mercury-induced cardiovascular diseases.<\/jats:p>","DOI":"10.1177\/1091581809338077","type":"journal-article","created":{"date-parts":[[2009,6,22]],"date-time":"2009-06-22T13:45:29Z","timestamp":1245678329000},"page":"190-206","source":"Crossref","is-referenced-by-count":20,"title":["Calcium and Calmodulin Regulate Mercury-induced Phospholipase D Activation in Vascular Endothelial Cells"],"prefix":"10.1177","volume":"28","author":[{"given":"Alon","family":"Peltz","sequence":"first","affiliation":[{"name":"From the Lipid Signaling and Lipidomics Laboratory, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shariq I.","family":"Sherwani","sequence":"additional","affiliation":[{"name":"From the Lipid Signaling and Lipidomics Laboratory, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sainath R.","family":"Kotha","sequence":"additional","affiliation":[{"name":"From the Lipid Signaling and Lipidomics Laboratory, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica N.","family":"Mazerik","sequence":"additional","affiliation":[{"name":"From the Lipid Signaling and Lipidomics Laboratory, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth S.","family":"O\u2019Connor Butler","sequence":"additional","affiliation":[{"name":"From the Lipid Signaling and Lipidomics Laboratory, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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