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With this concern in mind, the present study investigated the potential effects of a hydroxytyrosol extract from olive mill waste (OMW) on urotoxicity induced by acute CP and BSO doses using a Swiss albino mouse model. Toxicity modulation was evaluated by measuring lipid peroxidation (LPO) and antioxidants in urinary bladder. The findings revealed that the hydroxytyrosol extract exerted a protective effect not only on LPO but also on enzymatic antioxidants. When compared to the controls, the CP-treated animals underwent significant decreases in the glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GP), and catalase (CAT) activities. The level of glutathione (GSH) was also reduced with increased doses of LPO in the CP-treated animals. L-Buthionine-SR-sulfoximine treatment exerted an additive toxic effect on the CP-treated animals. Interestingly, pretreatment with the hydroxytyrosol extract restored the activities of all enzymes back to normal levels and exhibited an overall protective effect on the CP- and BSO-induced toxicities in urinary bladder. The restoration of GSH through the treatment with the hydroxytyrosol extract can play an important role in reversing CP-induced apoptosis and free radical-mediated LPO.\u00a0<\/jats:p>","DOI":"10.1177\/1091581811410444","type":"journal-article","created":{"date-parts":[[2011,7,20]],"date-time":"2011-07-20T00:31:55Z","timestamp":1311121915000},"page":"419-427","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["In Vivo Prevention of Bladder Urotoxicity"],"prefix":"10.1177","volume":"30","author":[{"given":"Kamel","family":"Rouissi","sequence":"first","affiliation":[{"name":"Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of El-Manar I, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bechr","family":"Hamrita","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of El-Manar I, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soumaya","family":"Kouidi","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of El-Manar I, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yosra","family":"Messai","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of El-Manar I, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bassem","family":"Jaouadi","sequence":"additional","affiliation":[{"name":"Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax, University of Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khaled","family":"Hamden","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, Sfax, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imen","family":"Medimegh","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of El-Manar I, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Slah","family":"Ouerhani","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Cherif","sequence":"additional","affiliation":[{"name":"Department of Urology, Charles Nicole Hospital, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amel Benammar","family":"Elgaaied","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, University of El-Manar I, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,7,19]]},"reference":[{"issue":"8","key":"bibr1-1091581811410444","doi-asserted-by":"crossref","first-page":"555","DOI":"10.2174\/1381612805666230110214512","volume":"5","author":"Colvin OM","year":"1999","journal-title":"Curr Pharm Des"},{"key":"bibr2-1091581811410444","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(01)01007-X"},{"key":"bibr3-1091581811410444","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(96)00200-X"},{"key":"bibr4-1091581811410444","doi-asserted-by":"crossref","unstructured":"Reliene R, Schiestl RH. 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