{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:01:22Z","timestamp":1784293282813,"version":"3.55.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Toxicol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>Our recent studies have shown that trichloroethylene (TCE) was able to induce multisystem injuries in the form of occupational medicamentosa-like dermatitis, including skin, kidney, and liver damages. However, the role of complement activation in the immune-mediated liver injury is not known. This study examined the role of complement activation in the liver injury in a mouse model of TCE-induced sensitization. Treatment of female BALB\/c mice with TCE under specific dosing protocols resulted in skin inflammation and sensitization. Skin edema and erythema occurred in TCE-sensitized groups. Trichloroethylene sensitization produced liver histopathological lesions, increased serum alanine aminotransferase, aspartate transaminase activities, and the relative liver weight. The concentrations of serum complement components C3a-desArg, C5a-desArg, and C5b-9 were significantly increased in 24-hour, 48-hour, and 72-hour sensitization-positive groups treated with TCE and peaked in the 72-hour sensitization-positive group. Depositions of C3a, C5a, and C5b-9 into the liver tissue were also revealed by immunohistochemistry. Immunofluorescence further verified high C5b-9 expression in 24-hour, 48-hour, and 72-hour sensitization-positive groups in response to TCE treatment. Reverse transcription\u2013polymerase chain reaction detected C3 messenger RNA expression in the liver, and this was significantly increased in 24-hour and 48-hour sensitization-positive groups with a transient reduction at 72 hours. These results provide the first experimental evidence that complement activation may play a key role in the generation and progression of immune-mediated hepatic injury by exposure to TCE.<\/jats:p>","DOI":"10.1177\/1091581813511337","type":"journal-article","created":{"date-parts":[[2013,11,7]],"date-time":"2013-11-07T22:11:40Z","timestamp":1383862300000},"page":"431-441","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["Complement Activation and Liver Impairment in Trichloroethylene-Sensitized BALB\/c Mice"],"prefix":"10.1177","volume":"32","author":[{"given":"Jiaxiang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wansheng","family":"Zha","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhai","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"},{"name":"Institute of Dermatology, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengfan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"},{"name":"Institute of Dermatology, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Shen","sequence":"additional","affiliation":[{"name":"Institute of Dermatology, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhao","family":"Wu","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"},{"name":"Institute of Dermatology, Anhui Medical University, Hefei, Anhui, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,11,7]]},"reference":[{"key":"bibr1-1091581813511337","unstructured":"IARC. 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