{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:09:34Z","timestamp":1787069374952,"version":"build-2736575974"},"reference-count":42,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T00:00:00Z","timestamp":1435190400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Toxicol"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>\n                    Biological oxidative responses are involved in the toxicity of multiwall carbon nanotubes (MWCNTs), which may cause asbestos-like pathogenicity. Superoxide dismutase 2 (SOD-2) has been proposed as a biomarker of early responses to mesothelioma-inducing fibers. This study was conducted to investigate the alteration of SOD-2 expression in the human mesothelial cell lines Met-5A after exposure to nontoxic doses of MWCNTs and the potential signaling pathway. The parameters measured included the viability, morphological change, superoxide formation, extracellular signal-regulated kinase 1\/2 (ERK1\/2) phosphorylation, and messenger RNA (mRNA)\/protein levels of SOD-2. Our results showed that MWCNTs upregulated SOD-2 expression at both mRNA and protein level. Coincidently, both superoxide formation and ERK1\/2 phosphorylation were observed in Met-5A cells exposed to MWCNTs and were diminished by pretreatment with the reactive oxidative species (ROS) scavenger, N-acetyl-\n                    <jats:sc>l<\/jats:sc>\n                    -(+)-cysteine (NAC). To further investigate the role of ROS\/ERK1\/2 in MWCNTs-induced SOD-2 overexpression, prior to MWCNTs exposure, cells were pretreated with the Mitogen-activated protein kinase kinase 1\/2 (MEK 1\/2) inhibitor (U0126) or with NAC. Both pretreatments decreased the MWCNTs-induced overexpression of SOD-2. These results suggest that upregulation of SOD-2 in Met-5A cells exposed to MWCNTs is mediated by ROS formation and ERK1\/2 activation.\n                  <\/jats:p>","DOI":"10.1177\/1091581815591223","type":"journal-article","created":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T23:01:22Z","timestamp":1435273282000},"page":"17-26","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["MWCNTs Induce ROS Generation, ERK Phosphorylation, and SOD-2 Expression in Human Mesothelial Cells"],"prefix":"10.1177","volume":"35","author":[{"given":"Min","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Occupational Diseases, Zhejiang Academy of Medical Sciences, Zhejiang, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Zhejiang Academy of Medical Sciences, Zhejiang, P. R. 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