{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T18:42:30Z","timestamp":1780080150173,"version":"3.54.0"},"reference-count":24,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100008335","name":"Arizona Biomedical Research Commission","doi-asserted-by":"publisher","award":["ADHS18-198880"],"award-info":[{"award-number":["ADHS18-198880"]}],"id":[{"id":"10.13039\/100008335","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007048","name":"Mayo Foundation for Medical Education and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007048","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U54 AI117804"],"award-info":[{"award-number":["U54 AI117804"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Phoenix Children's Hospital Foundation"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Pediatr Dev Pathol"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diagnosis of non-esophageal eosinophilic gastrointestinal disorders requires quantification of tissue eosinophils. Our objective was to evaluate eosinophil peroxidase (EPX) immunohistochemistry (IHC) as a method for histologic diagnosis of eosinophilic gastritis (EG) and eosinophilic duodenitis (EoD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We performed a retrospective analysis of biopsies from pediatric EG\/EoD cases and controls. Subjects with EG or EoD had \u226530 eosinophils per high power field (eos\/hpf) in \u22655\u2009hpf in the stomach and\/or \u22653\u2009hpf in the duodenum, respectively. Controls had no histopathologic diagnosis recorded. Tissue eosinophil counts were assessed by hematoxylin &amp; eosin stains. EPX stains were assessed using a unique histopathologic scoring system. Slides were digitized and EPX+ staining area\/mm\n                      <jats:sup>2<\/jats:sup>\n                      was quantified by image analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty-six EG\/EoD cases and 40 controls were analyzed. EPX scores and EPX\/mm\n                      <jats:sup>2<\/jats:sup>\n                      levels were markedly elevated in EG\/EoD (p\u2009\u2264\u20090.0001). Eosinophil density (eos\/mm\n                      <jats:sup>2<\/jats:sup>\n                      ) correlated strongly with EPX scores and EPX\/mm\n                      <jats:sup>2<\/jats:sup>\n                      levels in the stomach (r\u2009\u2265\u20090.77) and moderately with EPX scores and EPX\/mm\n                      <jats:sup>2<\/jats:sup>\n                      levels in the duodenum (r\u2009\u2265\u20090.52); (p\u2009&lt;\u20090.0001). EPX quantification identified EG\/EoD subjects with high diagnostic accuracy (EPX score: AUC\u2009=\u20091 for EG and EoD; EPX\/mm\n                      <jats:sup>2<\/jats:sup>\n                      : AUC\u2009=\u20090.98 (95%CI 0.96-1) for EG, AUC\u2009=\u20090.91 (95%CI 0.81-1) for EoD).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>EPX-based assessment of eosinophilic inflammation may facilitate automated histologic diagnosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/10935266211024552","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T03:18:28Z","timestamp":1624850308000},"page":"513-522","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Diagnosis of Pediatric Non-Esophageal Eosinophilic Gastrointestinal Disorders by Eosinophil Peroxidase Immunohistochemistry"],"prefix":"10.1177","volume":"24","author":[{"given":"Shaina H","family":"Hasan","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Allergy, Asthma and Clinical Immunology, Mayo Clinic Arizona, Mayo Clinic Arizona, Scottsdale, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Phoenix Children\u2019s Hospital, Phoenix, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4967-9056","authenticated-orcid":false,"given":"Shipra","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Phoenix Children\u2019s Hospital, Phoenix, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R","family":"Buras","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Research, Division of Biomedical Statistics and Biomedical Informatics, Mayo Clinic Arizona, Scottsdale, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred D","family":"Doyle","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy, Asthma and Clinical Immunology, Mayo Clinic Arizona, Mayo Clinic Arizona, Scottsdale, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy S","family":"Bauer","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Section of Allergy and Immunology, Phoenix Children\u2019s Hospital, Phoenix, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7586-4722","authenticated-orcid":false,"given":"Benjamin L","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy, Asthma and Clinical Immunology, Mayo Clinic Arizona, Mayo Clinic Arizona, Scottsdale, Arizona"},{"name":"Department of Pulmonology, Section of Allergy and Immunology, Phoenix Children\u2019s Hospital, Phoenix, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shauna","family":"Schroeder","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Phoenix Children\u2019s Hospital, Phoenix, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"bibr1-10935266211024552","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.10.047"},{"key":"bibr2-10935266211024552","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2018.07.009"},{"key":"bibr3-10935266211024552","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2017.00261"},{"key":"bibr4-10935266211024552","doi-asserted-by":"publisher","DOI":"10.1016\/j.giec.2017.07.001"},{"key":"bibr5-10935266211024552","first-page":"36","volume":"188","author":"Kaijser R.","year":"1937","journal-title":"Arch Klin 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