{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T11:13:00Z","timestamp":1780917180498,"version":"3.54.1"},"reference-count":20,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas","doi-asserted-by":"crossref","award":["A1-S-8288"],"award-info":[{"award-number":["A1-S-8288"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:p>\n                    The antibacterial effect of chloramphenicol, hibiscus acid, and a mixture of hibiscus acid and chloramphenicol against antibiotic-resistant enterohemorrhagic\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    (EHEC) and\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Typhimurium (ST) was determined in Caesarean-Derived (CD)-1 mice. Hibiscus acid was isolated from\n                    <jats:italic toggle=\"yes\">Hibiscus sabdariffa<\/jats:italic>\n                    calyces. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of chloramphenicol (CH), hibiscus acid (HA), and mixtures of HA\/CH were determined for EHEC and ST. 11 groups of six mice each were formed. Four groups were inoculated orally with 4 log\n                    <jats:sub>10<\/jats:sub>\n                    Colony forming units (CFU) of ST, four groups were inoculated with 4 log\n                    <jats:sub>10<\/jats:sub>\n                    CFU of EHEC, and the remaining three groups were not inoculated. Six hours post inoculation, the mice in some groups received, via the oral route, solutions of hibiscus acid (7 mg\/mL), chloramphenicol (82 \u00b5g\/mL), a mixture of HA\/CH (5.7 mg\/mL HA and 4 \u00b5g\/mL CH), or isotonic saline solution. The MIC and MBC values were between 7 and 5 mg\/mL for hibiscus acid, between 17.6 and 82 \u00b5g\/mL for chloramphenicol, and between 4.2 mg\/mL\/0.3 \u00b5g\/mL and 5.7 mg\/mL\/0.4 \u00b5g\/mL (concentration of hibiscus acid\/concentration of chloramphenicol) or HA\/CH. EHEC and ST were not detected in the feces of mice that were administered hibiscus acid alone or in mixture with chloramphenicol. By contrast, pathogens were isolated from the feces of untreated mice and those treated with chloramphenicol alone throughout the study.\n                  <\/jats:p>","DOI":"10.1177\/1096620x251384555","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T16:19:49Z","timestamp":1759249189000},"page":"379-383","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibacterial Activity of Hibiscus Acid Alone and Combined with Chloramphenicol in CD-1 Mice Infected with Multidrug-Resistant Enterohemorrhagic\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Salmonella<\/i>\n                    Typhimurium"],"prefix":"10.1177","volume":"29","author":[{"given":"Lizbeth Anah\u00ed","family":"Portillo-Torres","sequence":"first","affiliation":[{"name":"Department of Biochemical Engineering, Celaya Institute of Technology, Celaya, 38010, Mexico City."},{"name":"Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mineral de la Reforma, 42183, Mexico City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Mercado-Monroy","sequence":"additional","affiliation":[{"name":"Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mineral de la Reforma, 42183, Mexico City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurea","family":"Bernardino-Nicanor","sequence":"additional","affiliation":[{"name":"Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mineral de la Reforma, 42183, Mexico City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto","family":"G\u00f3mez-Aldapa","sequence":"additional","affiliation":[{"name":"Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mineral de la Reforma, 42183, Mexico City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leopoldo","family":"Gonz\u00e1lez-Cruz","sequence":"additional","affiliation":[{"name":"Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mineral de la Reforma, 42183, Mexico City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7863-2911","authenticated-orcid":false,"given":"Esmeralda","family":"Rangel-Vargas","sequence":"additional","affiliation":[{"name":"Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mineral de la Reforma, 42183, Mexico City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7823-8954","authenticated-orcid":false,"given":"Javier","family":"Castro-Rosas","sequence":"additional","affiliation":[{"name":"Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mineral de la Reforma, 42183, Mexico City."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,9,30]]},"reference":[{"key":"e_1_3_4_2_2","unstructured":"World Health Organization. WHO releases report on state of development of antibacterials. Geneva Switzerland; 2019. Available from: https:\/\/www.who.int\/news\/item\/14-06-2024-who-releases-report-on-state-of-development-of-antibacterials [Last accessed: August23 2025]."},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics8040218"},{"issue":"2","key":"e_1_3_4_4_2","first-page":"109","article-title":"Preliminary screening of antibacterial activity using crude extracts of Hibiscus rosa sinensis","volume":"20","author":"Arullappan S","year":"2009","unstructured":"Arullappan S, , Zakaria Z, , Basri DF. Preliminary screening of antibacterial activity using crude extracts of Hibiscus rosa sinensis. Trop Life Sci Res, 2009; 20(2):109\u2013118.","journal-title":"Trop Life Sci Res"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7059323"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1259-3"},{"issue":"3","key":"e_1_3_4_7_2","first-page":"41","article-title":"Surveillance for foodborne disease outbreaks\u2013United States, 2009-2010","volume":"62","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. Surveillance for foodborne disease outbreaks\u2013United States, 2009-2010. MMWR Morb Mortal Wkly Rep, 2013; 62(3):41\u201347.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2301.160771"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2015.08.020"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.5863\/1551-6776-17.2.182"},{"issue":"2","key":"e_1_3_4_11_2","first-page":"515","article-title":"Roselle (Hibiscus sabdariffa L.) as a source of natural colour: A review","volume":"16","author":"Shruthi VH","year":"2016","unstructured":"Shruthi VH, , Ramachandra CT, , Nidoni U, et al. Roselle (Hibiscus sabdariffa L.) as a source of natural colour: A review. Plant Arch, 2016; 16(2):515\u2013522.","journal-title":"Plant Arch"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2020.0207"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2020.0216"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.17221\/105\/2023-VETMED"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12320"},{"key":"e_1_3_4_16_2","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2020","unstructured":"Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. 30th ed.. 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