{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:04:00Z","timestamp":1789977840883,"version":"4.0.1"},"reference-count":31,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Secretary of Science, Humanities, Technology and Innovation","award":["CBF2023-2024-387"],"award-info":[{"award-number":["CBF2023-2024-387"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"abstract":"<jats:p>\n                    The aim of this study was to assess the\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    larvicidal activity of hydroalcoholic extracts (HAE) from four\n                    <jats:italic toggle=\"yes\">Pleurotus djamor<\/jats:italic>\n                    fungal strains against\n                    <jats:italic toggle=\"yes\">Haemonchus contortus<\/jats:italic>\n                    infective larvae (L3) and to evaluate the associated structural alterations in the larval cuticle using scanning electron microscopy (SEM) as an alternative to chemical anthelmintics. HAE were obtained from fruiting bodies through maceration. Larvae\/HAE confrontation was carried out in 96 well ELISA plates. Two similar experiments were simultaneously performed to assess the HAE activity against sheathed (ShL3) and exsheathed (ExshL3)\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    larvae, respectively. Six well series (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 4) were confirmed in every assessment. Each well contained 200\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    (L3) in 20 \u03bcL of distilled water plus the corresponding HAE as follows: Series 1) Negative control (Distilled water 80 \u03bcL); Series 2) Positive control (Ivermectin 10 mg\/mL); Series 3)\n                    <jats:italic toggle=\"yes\">P. djamor<\/jats:italic>\n                    ECS-0142 strain (Pd-ECS-0142); Series 4)\n                    <jats:italic toggle=\"yes\">P. djamor<\/jats:italic>\n                    ECS-0143 strain (Pd-ECS-0143); Series 5)\n                    <jats:italic toggle=\"yes\">P. djamor<\/jats:italic>\n                    ECS-0127 strain (Pd-ECS-0127); Series 6)\n                    <jats:italic toggle=\"yes\">P. djamor<\/jats:italic>\n                    ECS-0123 strain (Pd-ECS-0123). The different fungal HAE were assessed at the following concentrations: 20, 40, 80, and 175 mg\/mL. Proportions of recovered larvae and larval mortality percentage were estimated, and the medium lethal concentration (LC\n                    <jats:sub>50<\/jats:sub>\n                    ) was also estimated. Pd-ECS-0127, Pd-ECS-0142, and Pd-ECS-0143 HAE showed mortality percentages lower than 50%. In contrast, Pd-ECS-0123 HAE showed an\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    larvicidal activity ranging between 90 and 100% against both ShL3 and ExshL3\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    (L3) after 72 h post-confrontation. LC\n                    <jats:sub>50<\/jats:sub>\n                    values of the fungal HAE with ShL3 and ExshL3 showed variability ranging from 71.30 to 167.5 mg\/mL. The exposure of larvae to the Pd-ECS-0123 HAE at 175 mg\/mL produced changes in the normal structural integrity of larvae.\n                  <\/jats:p>","DOI":"10.1177\/1096620x261489402","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T08:45:43Z","timestamp":1789721143000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>In Vitro<\/i>\n                    Larvicidal Activity of Hydroalcoholic Extracts from Four\n                    <i>Pleurotus djamor<\/i>\n                    Fungal Strains Against\n                    <i>Haemonchus contortus<\/i>"],"prefix":"10.1177","author":[{"given":"Samantha B.","family":"Rodr\u00edguez-B\u00e1maca","sequence":"first","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias (INIFAP), Sede Palo, Ciudad de M\u00e9xico, M\u00e9xico."},{"name":"Departamento Ciencias de la Sustentabilidad, Colegio de la Frontera Sur, Tapachula, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Carre\u00f1o-Campos","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias (INIFAP), Sede Palo, Ciudad de M\u00e9xico, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 E.","family":"S\u00e1nchez-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Departamento Ciencias de la Sustentabilidad, Colegio de la Frontera Sur, Tapachula, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia","family":"Moreno-Ruiz","sequence":"additional","affiliation":[{"name":"Departamento Ciencias de la Sustentabilidad, Colegio de la Frontera Sur, Tapachula, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge A.","family":"Maldonado-J\u00e1quez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias. Campo Experimental La Laguna, Matamoros, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Gonz\u00e1lez-Gardu\u00f1o","sequence":"additional","affiliation":[{"name":"Unidad Regional Universitaria Sursureste. Universidad Aut\u00f3noma de Chapingo. Teapa, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itzel B.","family":"Morales-Montesinos","sequence":"additional","affiliation":[{"name":"Colegio de Postgraduados, Campus Montecillo, Texcoco, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8944-5430","authenticated-orcid":false,"given":"Liliana","family":"Aguilar-Marcelino","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias (INIFAP), Sede Palo, Ciudad de M\u00e9xico, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11686-022-00600-x"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.5455\/jva.20130219032348"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2576-0"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.11.048"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X13000618"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2010.10.008"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920112800399121"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2023.122135"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02906801"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4269-2-3"},{"key":"e_1_3_3_12_1","volume-title":"Los Hongos Comestibles Silvestres Del Parque Nacional Izta-Popo, Zoquiapan y Anexos","author":"Per\u00e9z Morenos J","year":"2010","unstructured":"11.Per\u00e9z Morenos J, , Lorenzana Fern\u00e1ndez A, , Carrasco-Hern\u00e1ndez V, et al. Los Hongos Comestibles Silvestres Del Parque Nacional Izta-Popo, Zoquiapan y Anexos. Colegio de Postgraduados, SEMANART, CONACYT. Montecillo, Texcoco, Estado de M\u00e9xico; 2010."},{"key":"e_1_3_3_13_1","first-page":"297","volume-title":"Hongos Comestibles y Medicinales En Iberoam\u00e9rica","author":"Valencia del Toro G","year":"2012","unstructured":"12.Valencia del Toro G, , Gariin Aguilar AE. Propiedades Medicinales de Los Hongos Comestiles. In: S\u00e1nchez JE, , Mata G. (Eds.), Hongos Comestibles y Medicinales En Iberoam\u00e9rica. Tapachula, Chiapas, M\u00e9xico; 2012; pp. 297\u2013307."},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17050580"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.3923\/ppj.2006.173.177"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03175350"},{"key":"e_1_3_3_17_1","first-page":"142","volume-title":"El Cultivo de Setas Pleurotus Spp En M\u00e9xico","author":"Cayetano Catarino M","year":"2007","unstructured":"16.Cayetano Catarino M, , Mata G, , Bernab\u00e9 Gonz\u00e1lez T. Cultivo de Pleototus Ostratus y P. Djamor Sobre Dos Productos Agr\u00edcolas En Guerrero. In: El Cultivo de Setas Pleurotus Spp En M\u00e9xico. (Eds) S\u00e1nchez V\u00e1zquez Jos\u00e9 E, , Martinez Carrera Daniel, , Mata Gerardo, , Hermilio Leal Lara. El Colegio de La Frontera Sur. 2007; pp. 142\u2013156."},{"key":"e_1_3_3_18_1","first-page":"31","article-title":"Grupos de interesterilidad y productividad de cepas de Pleurotus de regiones tropicales y subtropicales de M\u00e9xico","volume":"30","author":"Huerta G","year":"2009","unstructured":"17.Huerta G, , Carrera DM, , S\u00e1nchez JE, et al. Grupos de interesterilidad y productividad de cepas de Pleurotus de regiones tropicales y subtropicales de M\u00e9xico. Sci Fungorum 2009;30:31\u201342; doi: 10.33885\/sf.2009.3.1052","journal-title":"Sci Fungorum"},{"key":"e_1_3_3_19_1","first-page":"141","volume-title":"La Biolog\u00eda y El Cultivo de Pleurotus Spp","author":"Quimio T","year":"2002","unstructured":"18.Quimio T. Preparaci\u00f3n de Semilla. In: S\u00e1nchez-V\u00e1zquez JE, , Royse DJ. (Eds.). La Biolog\u00eda y El Cultivo de Pleurotus Spp. El Colegio de La Frontera Sur\/Ed. Limusa, S.A. de C.V. M\u00e9xico, D.F., 2002; pp. 141\u2013156."},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X09990332"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1722"},{"key":"e_1_3_3_22_1","first-page":"26","volume-title":"Diagn\u00f3stico y Control de Los Nematodos Gastrointestinales de Los Rumiantes En M\u00e9xico","author":"Li\u00e9bano Hern\u00e1ndez E","year":"2004","unstructured":"21.Li\u00e9bano Hern\u00e1ndez E. Diagn\u00f3stico Coproparasitosc\u00f3pico de Los Nematodos Gastroent\u00e9ricos. In: Herrera-Rodr\u00edguez D, , Li\u00e9bano-Hern\u00e1ndez E, , L\u00f3pez-Arellano M.E, , Mendoza-de-Gives P, , V\u00e1zquez-Prats V. (Eds.), Diagn\u00f3stico y Control de Los Nematodos Gastrointestinales de Los Rumiantes En M\u00e9xico. Jiutepec, Morelos; 2004; pp. 26\u201366."},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.3329\/bpj.v18i1.23512"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1645\/13-377.1"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.574"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13131777"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/15572536.2005.11832870"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2017.0031"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2014.05.003"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.837266"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-7233-6"},{"key":"e_1_3_3_32_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6078917"}],"container-title":["Journal of Medicinal Food"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1096620X261489402","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1096620X261489402","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1096620X261489402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:18:30Z","timestamp":1789975110000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1096620X261489402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":31,"alternative-id":["10.1177\/1096620X261489402"],"URL":"https:\/\/doi.org\/10.1177\/1096620x261489402","relation":{},"ISSN":["1096-620X","1557-7600"],"issn-type":[{"value":"1096-620X","type":"print"},{"value":"1557-7600","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]},"article-number":"1096620X261489402"}}