{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:06:43Z","timestamp":1793052403706,"version":"4.5.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2016,7,10]],"date-time":"2016-07-10T00:00:00Z","timestamp":1468108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Feline Medicine and Surgery"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The objective of this study was to develop a quantitative 5' nuclease real-time polymerase chain reaction (PCR) assay to diagnose infections caused by\n                      <jats:italic toggle=\"yes\">Bartonella<\/jats:italic>\n                      species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Between January and April 2013 whole blood samples were collected by convenience from 151 cats (86 domiciled and 65 stray cats). The feline blood samples were subjected to a novel quantitative 5' nuclease real-time PCR (qPCR) for\n                      <jats:italic toggle=\"yes\">Bartonella<\/jats:italic>\n                      species targeting the nictonamide adenine dinucleotide dehydrogenase gamma subunit (\n                      <jats:italic toggle=\"yes\">nuoG<\/jats:italic>\n                      ) and conventional PCR assays targeting intergenic transcribed spacer,\n                      <jats:italic toggle=\"yes\">ribC, gltA, pap31<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                      , followed by sequencing and basic local alignment search tool analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The qPCR assay detected as few as 10 copies of plasmid per reaction. Forty-six (54.4% domiciled and 45.6% stray cats) of 151 sampled cats showed positive results in\n                      <jats:italic toggle=\"yes\">nuoG<\/jats:italic>\n                      qPCR for\n                      <jats:italic toggle=\"yes\">Bartonella<\/jats:italic>\n                      species. The absolute quantification of\n                      <jats:italic toggle=\"yes\">nuoG Bartonella<\/jats:italic>\n                      DNA in sampled cats ranged from 1.1 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      to 1.3 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      . Eighteen (39.1%) of 46 positive samples in the qPCR were also positive in conventional PCR assays. The sequencing confirmed that\n                      <jats:italic toggle=\"yes\">Bartonella henselae<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Bartonella clarridgeiae<\/jats:italic>\n                      circulate in cats in midwestern Brazil.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and relevance<\/jats:title>\n                    <jats:p>\n                      The present work provides details of a novel qPCR assay to diagnose infections caused by\n                      <jats:italic toggle=\"yes\">Bartonella<\/jats:italic>\n                      species.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1098612x15593787","type":"journal-article","created":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T10:54:19Z","timestamp":1435920859000},"page":"783-790","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":58,"title":["Assessment of a quantitative 5' nuclease real-time polymerase chain reaction using the nicotinamide adenine dinucleotide dehydrogenase gamma subunit (\n                    <i>nuoG<\/i>\n                    ) for\n                    <i>Bartonella<\/i>\n                    species in domiciled and stray cats in Brazil"],"prefix":"10.1177","volume":"18","author":[{"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"first","affiliation":[{"name":"Paulista State University (UNESP), Jaboticabal, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Stephen","family":"Dumler","sequence":"additional","affiliation":[{"name":"University of Maryland, Baltimore, MD, USA"},{"name":"Johns Hopkins School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heitor M","family":"Herrera","sequence":"additional","affiliation":[{"name":"Dom Bosco Catholic University, Campo Grande, Mato Grosso do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz R","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Paulista State University (UNESP), Jaboticabal, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyla CM","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Paulista State University (UNESP), Jaboticabal, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Gerardi","family":"Scorpio","sequence":"additional","affiliation":[{"name":"Ross University School of 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