{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T02:37:48Z","timestamp":1793932668032,"version":"4.8.0"},"reference-count":13,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T00:00:00Z","timestamp":1557792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Feline Medicine and Surgery"],"published-print":{"date-parts":[[2020,4]]},"abstract":"\n Objectives<\/jats:title>\n \n Diagnosis of\n Platynosomum fastosum<\/jats:italic>\n Kossack 1910, a liver fluke of cats, can be challenging, with infections potentially not identified depending on the fecal analysis method used. The aim of this study was to compare three methods for identification of\n P fastosum<\/jats:italic>\n eggs in feces.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Feces from 50 cats with unknown infection status from a location endemic for\n P fastosum<\/jats:italic>\n were analyzed. The methods used included double centrifugation with Sheather\u2019s sugar flotation solution (specific gravity 1.28); the Mini-Parasep (Apacor) flotation kit with ZnSO\n 4<\/jats:sub>\n (specific gravity 1.20); and the Mini-Parasep sedimentation kit with Triton X\/ethyl acetate solution.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Thirty-four cats were identified as positive for\n P fastosum<\/jats:italic>\n . Of these cats, 33 were identified with the double centrifugation method, 16 with the Mini-Parasep flotation kit and 11 with the Mini-Parasep sedimentation kit. Using the total number of samples identified as positive by at least one method as the true positive, the sensitivity of the methods was 97.1% for double centrifugation, 47.1% for the Mini-Parasep flotation kit and 32.4% for the Mini-Parasep sedimentation kit. Other parasite eggs (eg,\n Ancylostoma tubaeforme, Trichuris<\/jats:italic>\n species and\n Toxocara cati<\/jats:italic>\n ) also were detected more frequently with the double centrifugation method.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions and relevance<\/jats:title>\n \n Of the three methods tested, double centrifugation with Sheather\u2019s sugar flotation solution was the most effective in identifying cats with\n P fastosum<\/jats:italic>\n and other helminth eggs in their feces. The lower specific gravity of the flotation solution in the Mini-Parasep flotation kit likely influenced the results. A primary drawback of the Mini-Parasep sedimentation kit was the amount of sediment. While more sediment was examined than recommended in the manufacturer\u2019s instructions, this was still only a small portion of the total sediment. In addition, reading the slides took longer than with the other methods.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/1098612x19848173","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T11:48:55Z","timestamp":1557834535000},"page":"395-398","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Comparison of fecal analysis methods for the detection of\n Platynosomum fastosum<\/i>\n in naturally infected cats"],"prefix":"10.1177","volume":"22","author":[{"given":"Hilary","family":"Eisenbraun","sequence":"first","affiliation":[{"name":"One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, Basseterre, St Kitts, West Indies"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnan","family":"Alluwie","sequence":"additional","affiliation":[{"name":"One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, Basseterre, St Kitts, West Indies"},{"name":"Ngee Ann Polytechnic, School of Life Sciences and Chemical Technology, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Connolly","sequence":"additional","affiliation":[{"name":"One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, Basseterre, St Kitts, West Indies"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeev","family":"Nair","sequence":"additional","affiliation":[{"name":"One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, Basseterre, St Kitts, West Indies"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme G","family":"Verocai","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA"},{"name":"Current address: Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6351-3140","authenticated-orcid":false,"given":"Jennifer K","family":"Ketzis","sequence":"additional","affiliation":[{"name":"One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, Basseterre, St Kitts, West Indies"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,5,14]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.12.016"},{"key":"e_1_3_3_3_2","first-page":"228","article-title":"The species of Platynosomum in felines","volume":"40","author":"Purvis GB","year":"1931","unstructured":"Purvis GB. The species of Platynosomum in felines. Vet Rec 1931; 40: 228\u2013229.","journal-title":"Vet Rec"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-3926-5"},{"key":"e_1_3_3_5_2","first-page":"90","article-title":"Evaluation of fecal technics for the diagnosis of cat liver fluke infection","volume":"26","author":"Palumbo NE","year":"1976","unstructured":"Palumbo NE, Taylor D, Perri SF. Evaluation of fecal technics for the diagnosis of cat liver fluke infection. Lab Anim Sci 1976; 26: 90\u2013493.","journal-title":"Lab Anim Sci"},{"key":"e_1_3_3_6_2","first-page":"890","article-title":"Evaluation of a commercial stool concentrator kit compared to direct smear and formalin-ethyl acetate concentration methods for diagnosis of parasitic infection with special reference to Opisthorchis viverrini sensu lato in Thailand","volume":"47","author":"Laoprom N","year":"2016","unstructured":"Laoprom N, Laithavewat L, Kopolrat K, et al. Evaluation of a commercial stool concentrator kit compared to direct smear and formalin-ethyl acetate concentration methods for diagnosis of parasitic infection with special reference to Opisthorchis viverrini sensu lato in Thailand. Southeast Asian J Trop Med Public Health 2016; 47: 890\u2013900.","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.5604\/12321966.1234003"},{"key":"e_1_3_3_8_2","first-page":"3","article-title":"Gastrointestinal parasites: the practice guide to accurate diagnosis and treatment","volume":"28","author":"Dryden MW","year":"2006","unstructured":"Dryden MW, Payne PA, Ridley RK, et al. Gastrointestinal parasites: the practice guide to accurate diagnosis and treatment. Comp Contin Educ Pract Vet 2006; 28: 3\u201313.","journal-title":"Comp Contin Educ Pract Vet"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.04.033"},{"key":"e_1_3_3_10_2","first-page":"3","article-title":"Fecal examination for the diagnosis of parasitism","author":"Zajac AM","year":"2012","unstructured":"Zajac AM, Conboy GA. Fecal examination for the diagnosis of parasitism. In: Zajac AM, Coboy GA (eds). Veterinary clinical parasitology. 8th ed. Chichester: Wiley-Blackwell, 2012, pp 3\u2013170.","journal-title":"Veterinary clinical parasitology"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.04.022"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782007000300051"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-015-4763-x"},{"key":"e_1_3_3_14_2","first-page":"229","article-title":"Evaluation of oral administration of ursodeoxycholic acid (AUDC) in the diagnosis of natural infection in cats Platynosomum illiciens [article in Portuguese]","volume":"33","author":"Leal PDS","year":"2011","unstructured":"Leal PDS, Campos DP, Rodrigues MLA, et al. Evaluation of oral administration of ursodeoxycholic acid (AUDC) in the diagnosis of natural infection in cats Platynosomum illiciens [article in Portuguese]. Braz J Vet Med 2011; 33: 229\u2013233.","journal-title":"Braz J Vet Med"}],"container-title":["Journal of Feline Medicine and Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1098612X19848173","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1098612X19848173","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1098612X19848173","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:29:28Z","timestamp":1792589368000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1098612X19848173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,14]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1177\/1098612X19848173"],"URL":"https:\/\/doi.org\/10.1177\/1098612x19848173","relation":{},"ISSN":["1098-612X","1532-2750"],"issn-type":[{"value":"1098-612X","type":"print"},{"value":"1532-2750","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,14]]}}}