{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:08:34Z","timestamp":1784095714436,"version":"3.55.0"},"reference-count":19,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100005722","name":"ludwig-maximilians-universit\u00e0t m\u00f1nchen","doi-asserted-by":"publisher","award":["VetResearch Program"],"award-info":[{"award-number":["VetResearch Program"]}],"id":[{"id":"10.13039\/501100005722","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Feline Medicine and Surgery"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Inhalation chambers are commonly used for the delivery of aerosol drugs to cats with respiratory disease. The aim of the study was to identify successful cleaning methods for inhalation devices after standardised bacterial contamination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Spacer devices of two different manufacturers were used: RC Chamber (Cegla Medizintechnik) and Aerokat (Trudell Medical International). The bacterial contamination was performed using\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      . Previously marked areas of the chamber were contaminated with 50\u2009\u03bcl of bacterial solution, containing between 2.2\u2009\u00d710\n                      <jats:sup>5<\/jats:sup>\n                      and 2.1\u2009\u00d710\n                      <jats:sup>8<\/jats:sup>\n                      colony-forming units\/ml each. After cleaning the devices as recommended by each manufacturer (RC Chamber: special microwave cleaning bag [n\u2009=\u20095] or boiling water with liquid dish detergent for 15 mins [n\u2009=\u20095]; Aerokat: rinsing in a solution of lukewarm water and liquid dish detergent for 15 mins), chambers were air-dried for 24 h and samples for bacterial culture were taken from three defined areas. Sample material was applied on M\u00fcller\u2013Hinton agar plates and subsequently incubated for 24\u2009h at 37\u00b0C.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Bacterial contamination was not detected in any of the examined inhalation devices using the recommended cleaning methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and relevance<\/jats:title>\n                    <jats:p>If inhalation chambers are cleaned following the manufacturers\u2019 recommendations, successful bacterial decontamination can be expected.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1098612x20913352","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T10:47:43Z","timestamp":1585651663000},"page":"181-184","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of different cleaning methods for feline inhalation chambers after bacterial contamination"],"prefix":"10.1177","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2751-2199","authenticated-orcid":false,"given":"Friederike K","family":"Klenk","sequence":"first","affiliation":[{"name":"Clinic of Small Animal Medicine, LMU University of Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"DeSimoi","sequence":"additional","affiliation":[{"name":"Clinic of Small Animal Medicine, LMU University of Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Wolf","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1785-6206","authenticated-orcid":false,"given":"Bianka S","family":"Schulz","sequence":"additional","affiliation":[{"name":"Clinic of Small Animal Medicine, LMU University of Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,3,31]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.4187\/respcare.04491504","article-title":"Nebulizer use and maintenance by cystic fibrosis patients: a survey study.","volume":"49","author":"Lester MK","year":"2004","unstructured":"Lester MK, Flume PA, Gray SL, et al. Nebulizer use and maintenance by cystic fibrosis patients: a survey study. Respir Care 2004; 49: 1504\u20131508.","journal-title":"Respir Care"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2214.2006.00669.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(84)90520-5"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.2001.1083"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2010.07.012"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2013.08.006"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12133"},{"key":"e_1_3_3_9_2","first-page":"584","article-title":"Home-use nebulizers: a potential primary source of Burkholderia cepaica and other colistin-resistant, gram negative bacteria in patients with cystic fibrosis.","volume":"1996","author":"Hutchinson GR","unstructured":"Hutchinson GR, Parker S, Pryor JA, et al. Home-use nebulizers: a potential primary source of Burkholderia cepaica and other colistin-resistant, gram negative bacteria in patients with cystic fibrosis. J Clin Microbiol 1996: 584\u2013587.","journal-title":"J Clin Microbiol"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/inpract.29.3.138"},{"key":"e_1_3_3_11_2","unstructured":"Trudell Medical International Animal Health Resources. Instructions. https:\/\/www.trudellmed.com\/sites\/default\/files\/inline-files\/IFU_AeroKat_AeroDawg.pdf (2015 accessed September 15 2019)."},{"key":"e_1_3_3_12_2","unstructured":"Cegla Medizintechnik RC-Animal-Chamber-Katze. RC-Animal-Chamber-Gebrauchsanweisung [article in German]. https:\/\/www.cegla.de\/files\/cegla-de\/downloads\/manuals\/de\/rc-animal-chamber-manual-de.pdf (accessed September 15 2019)."},{"key":"e_1_3_3_13_2","unstructured":"Cegla Medizintechnik RC-Clean Reinigungsbeutel. Cleaning with RC-Clean bag. https:\/\/www.cegla.de\/files\/cegla-de\/downloads\/manuals\/RC-Clean-manual.pdf (accessed September 15 2019)."},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1590\/1806-3713\/e20170351"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/1479972317707652"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2005.06.001"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1806-37132014000200004"},{"key":"e_1_3_3_18_2","unstructured":"Cegla Medizintechnik RC-Animal-Chamber-Katze. RC-Animal-Chamber-Flyer [article in German]. https:\/\/www.cegla.de\/files\/cegla-de\/downloads\/flyer\/de\/rc-animal-chamber-flyer-de.pdf (accessed February 10 2020)."},{"key":"e_1_3_3_19_2","unstructured":"Trudell Medical International Animal Health Resources. Dosing information. https:\/\/www.trudellmed.com\/sites\/default\/files\/inline-files\/aerokat-dosing-information.pdf (2015 accessed February 10 2020)."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12541"}],"container-title":["Journal of Feline Medicine and Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1098612X20913352","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1098612X20913352","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1098612X20913352","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T05:52:36Z","timestamp":1784094756000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1098612X20913352"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,31]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1177\/1098612X20913352"],"URL":"https:\/\/doi.org\/10.1177\/1098612x20913352","relation":{},"ISSN":["1098-612X","1532-2750"],"issn-type":[{"value":"1098-612X","type":"print"},{"value":"1532-2750","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,31]]}}}