{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:07:05Z","timestamp":1784095625147,"version":"3.55.0"},"reference-count":50,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T00:00:00Z","timestamp":1615766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T00:00:00Z","timestamp":1615766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Feline Medicine and Surgery"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Recent studies show that cats could play an important role in the transmission of\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      species. There are few reports of leptospirosis on Prince Edward Island (PEI) and none in cats. The objective of this study was to determine the prevalence of serum antibodies against\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      serovars and of\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      DNA in the urine of a population of free-roaming cats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Paired blood and urine samples were collected from 200 cats brought to a trap\u2013neuter\u2013return program. Antibody titers against six\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      serovars (Bratislava, Canicola, Gryppotyphosa, Hardjo, Pomona, Icterohaemorrhagiae) were determined by microscopic agglutination test. PCR was performed on urine samples to identify urine shedding of\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      DNA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Antibodies were detected in 20\/200 cats (10%) for at least one serovar, with titers ranging from 1:50 to 1:6400 (all serovars tested, except Hardjo). Urine samples of 7\/200 cats (3.5%) were PCR-positive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and relevance<\/jats:title>\n                    <jats:p>\n                      Feral cats in PEI had a higher than expected exposure to leptospirosis and can shed DNA from pathogenic\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      species in urine. Further studies are needed to determine the prevalence of exposure to leptospirosis in other species on PEI and the potential role of feral cats in transmission of the disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1098612x211001042","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T19:37:23Z","timestamp":1615923443000},"page":"1155-1161","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["Serologic and urinary survey of exposure to\n                    <i>Leptospira<\/i>\n                    species in a feral cat population of Prince Edward Island, Canada"],"prefix":"10.1177","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0213-928X","authenticated-orcid":false,"given":"Emilia","family":"Bourassi","sequence":"first","affiliation":[{"name":"Department of Companion Animals, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Savidge","sequence":"additional","affiliation":[{"name":"Department of Companion Animals, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Foley","sequence":"additional","affiliation":[{"name":"Department of Companion Animals, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunny","family":"Hartwig","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,3,15]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03474.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2208"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2007.09.011"},{"key":"e_1_3_2_7_2","first-page":"237","article-title":"Leptospirosis worldwide.","volume":"74","author":"World Health Organization","year":"1999","unstructured":"World Health Organization. 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