{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:55:35Z","timestamp":1792799735793,"version":"4.5.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T00:00:00Z","timestamp":1655251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T00:00:00Z","timestamp":1655251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"University of Edinburgh internal start-up fund","id":[{"id":"https:\/\/ror.org\/01nrxwf90","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Feline Medicine and Surgery"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Current blood tests to diagnose feline liver diseases are suboptimal. Serum concentrations of microRNA (miR)-122 have been shown in humans, dogs and rodents to be a sensitive and specific biomarker for liver injury. To explore the potential diagnostic utility of measuring serum concentrations of miR-122 in cats, miR-122 was measured in a cohort of ill, hospitalised cats with known serum alanine aminotransferase (ALT) activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this retrospective study, cats were grouped into those with an ALT activity within the reference interval (0\u201383\u2009U\/l; n\u2009=\u200938) and those with an abnormal ALT activity (&gt;84\u2009U\/l; n\u2009=\u200925). Serum concentrations of miR-122 were measured by real-time quantitative PCR and the relationship between miR-122 and ALT was examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      miR-122 was significantly higher in the group with high ALT activity than the ALT group, within normal reference limits (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt;0.0004). There was also a moderately positive correlation between serum ALT activity and miR-122 concentrations (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt;0.001;\n                      <jats:italic toggle=\"yes\">r<\/jats:italic>\n                      \u2009=\u20090.52).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and relevance<\/jats:title>\n                    <jats:p>Concentrations of miR-122 were reliably quantified in feline serum and were higher in a cohort of cats with increased ALT activity than in cats with normal ALT activity. This work highlights the potential diagnostic utility of miR-122 as a biomarker of liver damage in cats and encourages further investigation to determine the sensitivity and specificity of miR-122 as a biomarker of hepatocellular injury in this species.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1098612x221100071","type":"journal-article","created":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T08:51:47Z","timestamp":1655283107000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of a relationship between serum concentrations of microRNA-122 and alanine aminotransferase activity in hospitalised cats"],"prefix":"10.1177","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5344-827X","authenticated-orcid":false,"given":"Susan K","family":"Armstrong","sequence":"first","affiliation":[{"name":"The Royal (Dick) School of Veterinary Studies and the Roslin Institute, The Hospital for Small Animals, University of Edinburgh, Edinburgh, UK"},{"name":"Pharmacology, Toxicology and Therapeutics, Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilna","family":"Oosthuyzen","sequence":"additional","affiliation":[{"name":"The Roslin Institute, University of Edinburgh, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam G","family":"Gow","sequence":"additional","affiliation":[{"name":"The Royal (Dick) School of Veterinary Studies and the Roslin Institute, The Hospital for Small Animals, University of Edinburgh, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Salavati Schmitz","sequence":"additional","affiliation":[{"name":"The Royal (Dick) School of Veterinary Studies and the Roslin Institute, The Hospital for Small Animals, University of Edinburgh, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W","family":"Dear","sequence":"additional","affiliation":[{"name":"Pharmacology, Toxicology and Therapeutics, Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J","family":"Mellanby","sequence":"additional","affiliation":[{"name":"The Royal (Dick) School of Veterinary Studies and the Roslin Institute, The Hospital for Small Animals, University of Edinburgh, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,6,15]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"Ettinger SJ Feldman EC. Textbook of veterinary internal medicine. 8th ed. St Louis MO: Elsevier 2017."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01299887"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fshw.2017.12.002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14716"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2018.667-673"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2007.tb01941.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.131.12.257"},{"key":"e_1_3_3_9_2","unstructured":"Feldman EC Nelson RW Rivera Mu\u00f1oz B et al. Canine and feline endocrinology and reproduction. 4th ed. Philadelphia: Elsevier 2014."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.5326\/15473317-37-3-228"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X18788883"},{"key":"e_1_3_3_12_2","first-page":"5","article-title":"Complications of ultrasound-guided liver biopsies in dogs and cats.","volume":"46","author":"Moritz A-K","year":"2018","unstructured":"Moritz A-K, K\u00f6hler C, Fromme V, et al. Complications of ultrasound-guided liver biopsies in dogs and cats. Tierarztl Prax Ausg K Kleintiere Heimtiere 2018; 46: 5\u201313.","journal-title":"Tierarztl Prax Ausg K Kleintiere Heimtiere"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020276"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.578193"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.106"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31012-1"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep05150"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1114519"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00809-6"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.1.2.1066"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15250"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26294"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1586\/17512433.2014.880650"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep15501"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(17)30266-2"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2016.01.010"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2014.03.015"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.03.027"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1997.211.02.188"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2016-0471"},{"key":"e_1_3_3_31_2","doi-asserted-by":"crossref","unstructured":"Bustin SA Benes V Garson JA et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Oxford: Oxford University Press 2009.","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-131"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000002864"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecns.2014.07.005"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.11.021"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2016.02.010"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29802"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules201019030"}],"container-title":["Journal of Feline Medicine and Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1098612X221100071","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1098612X221100071","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1098612X221100071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:19:43Z","timestamp":1792588783000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1098612X221100071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,15]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1177\/1098612X221100071"],"URL":"https:\/\/doi.org\/10.1177\/1098612x221100071","relation":{},"ISSN":["1098-612X","1532-2750"],"issn-type":[{"value":"1098-612X","type":"print"},{"value":"1532-2750","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,15]]}}}