{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:00:02Z","timestamp":1792800002506,"version":"4.5.0"},"reference-count":35,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Center for Companion Animal Health at the University of California, Davis School of Veterinary Medicine","id":[{"id":"https:\/\/ror.org\/05rrcem69","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Feline Medicine and Surgery"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Leptospirosis is a re-emergent zoonotic bacterial disease associated with renal and hepatic injury. In free-roaming cats in some regions, a high prevalence of Leptospira antibodies has been identified, and pathogenic leptospires have been detected in renal tissue, indicating that they may play a role in Leptospira epidemiology. The objective of this study was to determine the prevalence of Leptospira seroreactivity and urinary shedding of Leptospira DNA in free-roaming cats from northern California and southern Texas. A secondary objective was to compare the results of a point-of-care (POC) assay, designed to detect Leptospira antibodies, with the results of the microscopic agglutination test (MAT) when applied to serum samples from feral cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Specimens were obtained from free-roaming cats from northern California (n\u2009=\u200952; 2020) and southern Texas (n\u2009=\u200975; 2017). Leptospira quantitative PCR was performed on blood and urine specimens from Californian cats. Serum samples from Californian and Texan cats were subjected to MAT to categorize them as Leptospira antibody-positive or antibody-negative. The performance of the POC assay was assessed using the MAT as the gold standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Leptospira DNA was not detected in the blood or urine of any cats tested. The results of the MAT were positive in 17.3% (n\u2009=\u20099) of Californian cats and 10.7% (n\u2009=\u20098) of Texan cats ( P\u2009=\u20090.3). The median MAT titer was 1:100 (range 1:100\u20131:200) in Californian cats and 1:200 (range 1:100\u20131:800) in Texan cats. The POC assay was negative in all specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and relevance<\/jats:title>\n                    <jats:p>Free-roaming cats in California and Texas are exposed to Leptospira species and may have the potential to act as sentinel hosts. No cats had evidence of current infection, as determined using PCR on blood and urine specimens. The POC test did not reliably detect anti- Leptospira antibodies in these cats. The role of cats in the maintenance or shedding of pathogenic leptospires requires further investigation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1098612x231162471","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T09:10:03Z","timestamp":1679476203000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of\n                    <i>Leptospira<\/i>\n                    infection and exposure in free-roaming cat populations in northern California and southern Texas"],"prefix":"10.1177","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8758-2530","authenticated-orcid":false,"given":"Jamie F","family":"Sebastian","sequence":"first","affiliation":[{"name":"Veterinary Medical Teaching Hospital, University of California-Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3426-6352","authenticated-orcid":false,"given":"Krystle L","family":"Reagan","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology, University of California-Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tess","family":"Peavy","sequence":"additional","affiliation":[{"name":"Public Vets, Bloomington, IN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Italo B","family":"Zecca","sequence":"additional","affiliation":[{"name":"Texas A&M University School of Veterinary Medicine & Biomedical Sciences, College Station, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A","family":"Hamer","sequence":"additional","affiliation":[{"name":"Texas A&M University School of Veterinary Medicine & Biomedical Sciences, College Station, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane E","family":"Sykes","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology, University of California-Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"key":"bibr1-1098612X231162471","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0654.x"},{"key":"bibr2-1098612X231162471","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007499"},{"key":"bibr3-1098612X231162471","first-page":"431","volume-title":"Infectious diseases of the dog and cat","author":"Greene CE","year":"2012","edition":"4"},{"key":"bibr4-1098612X231162471","doi-asserted-by":"publisher","DOI":"10.5326\/JAAHA-MS-5748"},{"key":"bibr5-1098612X231162471","first-page":"115","volume":"49","author":"Beaudu-Lange C","year":"2014","journal-title":"Rev Vet 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