{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T08:32:41Z","timestamp":1794385961025,"version":"4.9.0"},"reference-count":67,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Mast Group Diagnostics"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Feline Medicine and Surgery"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>The aim of the present study was to evaluate the agreement of a commercially available point-of-care (POC) feline coronavirus (FCoV) quantitative RT-PCR (RT-qPCR) assay and external laboratories\u2019 RT-qPCR testing in cats with effusive and non-effusive feline infectious peritonitis (FIP), and in cats with other diseases presenting with similar clinical signs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Client-owned cats suspected of having FIP were enrolled with owner consent. Effusions and ultrasound-guided fine-needle aspirates (FNAs) from the liver, spleen or mesenteric lymph nodes were collected, split and analysed using both a POC RT-qPCR and an external laboratory RT-qPCR. FIP was diagnosed based on American Association of Feline Practitioners\/EveryCat guidelines. Sensitivity and negative predictive values were calculated, agreement assessed with Cohen\u2019s kappa, paired differences tested with McNemar\u2019s test and correlation of crossing point (Cp) values analysed using Pearson\u2019s coefficient and paired\n                      <jats:italic toggle=\"yes\">t<\/jats:italic>\n                      -tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>A total of 100 cats were included (effusions n\u2009=\u200962, FNAs n\u2009=\u200938). Overall agreement between assays was substantial (\u03ba\u2009=\u20090.73), with near-perfect concordance for effusions (\u03ba\u2009=\u20090.81) and lower agreement for FNAs (\u03ba\u2009=\u20090.61). Relative sensitivity was highest in effusions (98.1% POC, 92.3% external) and lower in FNAs (63.3\u201364.5%), with negative predictive values showing similar trends. Cp values were moderately correlated between assays, with minor mean differences (~1 cycle).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and relevance:<\/jats:title>\n                    <jats:p>The POC FCoV RT-qPCR assay is a practical and reliable tool and shows very good agreement with external reference laboratory RT-qPCR testing. FNAs remain useful when effusions are unavailable but carry a higher risk of false negatives, likely due to low viral loads and heterogeneous distribution. POC care testing offers rapid turnaround, supporting timely clinical decision-making, but results must be interpreted alongside clinical presentation and other diagnostics.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1098612x261483596","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T12:39:29Z","timestamp":1787229569000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Agreement between point-of-care and external reference laboratory RT-qPCR testing for feline coronavirus in cats with suspected feline infectious peritonitis"],"prefix":"10.1177","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4586-7025","authenticated-orcid":false,"given":"Marieke","family":"Knies","sequence":"first","affiliation":[{"name":"AniCura Specialist Referral Centre Haaglanden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikki","family":"Hofman","sequence":"additional","affiliation":[{"name":"AniCura Specialist Referral Centre Haaglanden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Jackson-Onis","sequence":"additional","affiliation":[{"name":"AniCura Specialist Referral Centre Haaglanden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1312-6115","authenticated-orcid":false,"given":"Els M.","family":"Broens","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7521-8173","authenticated-orcid":false,"given":"Erik","family":"Teske","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","unstructured":"Vogel L Van der Lubben M Te Lintelo EG et al. 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