{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T01:38:23Z","timestamp":1778895503130,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To elucidate the pathomechanism of Fuchs' dystrophy and pseudophakic bullous keratopathy (PBK) by examining cell apoptosis in different corneal layers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The authors studied corneal buttons obtained from 21 eyes following central penetrating keratoplasty: 14 corneal buttons (13 patients, age 70.8 \u00b1 10.0 years) with Fuchs' dystrophy, and 7 buttons (7 patients, age 69.6 \u00b1 10.2 years) with PBK. Four buttons from enucleated eyes with choroidal melanoma served as controls. Histologic changes were examined using light microscopy with hematoxylin-eosin (HE) staining. The average numbers of apoptotic cells per field of view (125x magnification) in separate samples of the epithelial, stromal, and endothelial layers were determined using the TUNEL (terminal deoxyri-bonucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling) assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In 11 of the Fuchs' dystrophy corneas and 2 of the PBK corneas, apoptotic activity was detected. In the control corneas no apoptotic activity was found. Compared to the controls there was a statistically significant difference in the mean (normalized) apoptotic cell numbers for all three layers (p=0.01 in each case) in the Fuchs' dystrophy corneas, and for the stromal layer (p&lt;0.01) in PBK corneas. The apoptotic cell numbers for the epithelial and endothelial layers of the latter were higher, but the difference was not statistically significant (p=0.07, 0.07).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Apoptosis may play a role in the pathomechanism of Fuchs' dystrophy and in keratocyte death in corneas with PBK.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/112067210501500103","type":"journal-article","created":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T00:45:06Z","timestamp":1516322706000},"page":"17-22","source":"Crossref","is-referenced-by-count":29,"title":["Epithelial Cell, Keratocyte, and Endothelial Cell Apoptosis in Fuchs' Dystrophy and in Pseudophakic Bullous Keratopathy"],"prefix":"10.1177","volume":"15","author":[{"given":"N.","family":"Szentm\u00e1ry","sequence":"first","affiliation":[{"name":"1st Department of Ophthalmology, Semmelweis University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Szende","sequence":"additional","affiliation":[{"name":"1st Department of Pathology and Experimental Cancer Research, Semmelweis University"},{"name":"Research Group for Molecular Pathology, Hungarian Academy of Sciences and Semmelweis University, Budapest - Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"S\u00fcveges","sequence":"additional","affiliation":[{"name":"1st Department of Ophthalmology, Semmelweis University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2005,1,1]]},"reference":[{"key":"bibr1-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1016\/0039-6257(93)90099-S"},{"key":"bibr2-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-198801000-00002"},{"key":"bibr3-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1982.01030040932013"},{"key":"bibr4-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1979.tb01831.x"},{"key":"bibr5-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(84)90015-0"},{"key":"bibr6-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1007\/s004390050580"},{"key":"bibr7-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)76616-4"},{"key":"bibr8-112067210501500103","first-page":"449","volume":"30","author":"Wilson S.E.","year":"1989","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr9-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(01)00008-8"},{"key":"bibr10-112067210501500103","first-page":"220","volume":"39","author":"Wilson S.E.","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr11-112067210501500103","first-page":"2501","volume":"41","author":"Borderie V.M.","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr12-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.119.11.1597"},{"key":"bibr13-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1002\/pros.1122"},{"key":"bibr14-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1999.0719"},{"key":"bibr15-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1997.13"},{"key":"bibr16-112067210501500103","first-page":"2151","volume":"36","author":"Rosenbaum J.T.","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr17-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0740"},{"key":"bibr18-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(00)00662-X"},{"key":"bibr19-112067210501500103","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1007\/s00347-002-0645-6","volume":"99","author":"Auw-Hadrich C.","year":"2002","journal-title":"Ophthalmologe"},{"key":"bibr20-112067210501500103","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.21.2415"}],"container-title":["European Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/112067210501500103","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/112067210501500103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T19:35:57Z","timestamp":1777404957000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/112067210501500103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1177\/112067210501500103"],"URL":"https:\/\/doi.org\/10.1177\/112067210501500103","relation":{},"ISSN":["1120-6721","1724-6016"],"issn-type":[{"value":"1120-6721","type":"print"},{"value":"1724-6016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1]]}}}