{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T19:58:46Z","timestamp":1777579126178,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To assess corneal tissue modifications after riboflavin-UVA-induced cross-linking of corneal collagen in patients with progressive keratoconus as well as regeneration of epithelium and subepithelial nerve plexus by in vivo HRT II system confocal microscopy in humans.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Ten patients with progressive keratoconus were treated by riboflavin-UVA-induced cross-linking of corneal collagen, involving assessment of ultrastructural modifications of the corneal epithelium and subepithelial nerve plexus by HRT II system confocal microscopy. Treatment included instillation of 0.1% riboflavin-20% dextrane solution 5 minutes before UVA irradiation and every 5 minutes for a total of 30 minutes. Radiant energy was 3 mW\/cm\n                      <jats:sup>2<\/jats:sup>\n                      or 5.4 Joule\/cm\n                      <jats:sup>2<\/jats:sup>\n                      and the source was dual UVA (370 nm) light-emitting led. The protocol included the operation followed by antibiotic medication and eye dressing with a soft therapeutic contact lens. Changes in epithelium and subepithelial and stromal nerve plexus were assessed by HRT II system confocal microscopy in vivo.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>After 5 days of soft contact lens wearing corneal epithelium has a regular morphology and density. Disappearance of subepithelial stromal nerve fibers was observed in the central irradiated area where, 1 month after the operation, initial reinnervation was microscopically observed. No changes in nerve fibers were observed in the peripheral untreated with a clear lateral transition between the two areas. Six months after the operation, the anterior subepithelial stroma was recolonized by nerve fibers with restoration of corneal sensitivity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>HRT II system confocal microscopy confirms corneal epithelium restore and reinnervation after riboflavin-UVA-induced collagen cross-linking directly in vivo in humans.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/112067210601600405","type":"journal-article","created":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T00:46:44Z","timestamp":1516322804000},"page":"530-535","source":"Crossref","is-referenced-by-count":57,"title":["Conservative Treatment of Keratoconus by Riboflavin-UVA-Induced Cross-Linking of Corneal Collagen: Qualitative Investigation of Corneal Epithelium and Subepithelial Nerve Plexus Regeneration by\n                    <i>in vivo<\/i>\n                    HRT II System Confocal Microscopy in Humans"],"prefix":"10.1177","volume":"16","author":[{"given":"C.","family":"Mazzotta","sequence":"first","affiliation":[{"name":"Department of Ophthalmological Sciences, Siena University, Siena - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Traversi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmological Sciences, Siena University, Siena - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Baiocchi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmological Sciences, Siena University, Siena - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Sergio","sequence":"additional","affiliation":[{"name":"Department of Ophthalmological Sciences, Siena University, Siena - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Caporossi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmological Sciences, Siena University, Siena - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Caporossi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmological Sciences, Siena University, Siena - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,7,1]]},"reference":[{"key":"bibr1-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(80)80027-3"},{"key":"bibr2-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(97)00119-7"},{"key":"bibr3-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.16.8.792.8989"},{"key":"bibr4-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.22.5.333.5491"},{"key":"bibr5-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1159\/000055492"},{"key":"bibr6-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199705000-00016"},{"key":"bibr7-112067210601600405","first-page":"220","volume":"39","author":"Wilson S.E.","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr8-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200203000-00017"},{"key":"bibr9-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199211000-00012"},{"key":"bibr10-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199409000-00005"},{"key":"bibr11-112067210601600405","first-page":"644","volume":"39","author":"Daxer A.","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr12-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(02)02220-1"},{"key":"bibr13-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1016\/S0886-3350(03)00407-3"},{"key":"bibr14-112067210601600405","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.87.2.225"},{"key":"bibr15-112067210601600405","first-page":"3660","volume":"43","author":"Lee B.H.","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr16-112067210601600405","first-page":"513","volume":"5","author":"Richter A.","year":"1996","journal-title":"Ger J Ophthalmol"}],"container-title":["European Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/112067210601600405","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/112067210601600405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T19:36:45Z","timestamp":1777405005000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/112067210601600405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1177\/112067210601600405"],"URL":"https:\/\/doi.org\/10.1177\/112067210601600405","relation":{},"ISSN":["1120-6721","1724-6016"],"issn-type":[{"value":"1120-6721","type":"print"},{"value":"1724-6016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7]]}}}