{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T23:11:58Z","timestamp":1793747518694,"version":"4.6.1"},"reference-count":25,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      To determine the effect of vascular endothelial growth factor (VEGF) Trap\n                      <jats:sub>R1R2<\/jats:sub>\n                      on bFGF-induced experimental corneal neovascularization (NV).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Control pellets or pellets containing 80 ng bFGF were surgically implanted into wild-type C57BL\/6 and VEGF-LacZ mouse corneas. The corneas were photographed, harvested, and the percentage of corneal NV was calculated. The harvested corneas were evaluated for VEGF expression. VEGF-LacZ mice received tail vein injections of an endothelial-specific lectin after pellet implantation to determine the temporal and spatial relationship between VEGF expression and corneal NV. Intraperitoneal injections of VEGF Trap\n                      <jats:sub>R1R2<\/jats:sub>\n                      or a human IgG Fc domain control protein were administered, and bFGF pellet-induced corneal NV was evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      NV of the corneal stroma began on day 4 and was sustained through day 21 following bFGF pellet implantation. Progression of vascular endothelial cells correlated with increased VEGF-LacZ expression. Western blot analysis showed increased VEGF expression in the corneal NV zone. Following bFGF pellet implantation, the area of corneal NV in untreated controls was 1.05\u00b10.12 mm\n                      <jats:sup>2<\/jats:sup>\n                      and 1.53\u00b10.27 mm\n                      <jats:sup>2<\/jats:sup>\n                      at days 4 and 7, respectively. This was significantly greater than that of mice treated with VEGF Trap (0.24\u00b10.11 mm\n                      <jats:sup>2<\/jats:sup>\n                      and 0.35\u00b10.16 mm\n                      <jats:sup>2<\/jats:sup>\n                      at days 4 and 7, respectively; p&lt;0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Corneal keratocytes express VEGF after bFGF stimulation and bFGF-induced corneal NV is blocked by intraperitoneal VEGF TrapR1R2 administration. Systemic administration of VEGF TrapR1R2 may have potential therapeutic applications in the management of corneal NV.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/112067211002000106","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T11:08:25Z","timestamp":1516792105000},"page":"48-54","source":"Crossref","is-referenced-by-count":32,"title":["VEGF Trap\n                    <sub>R1R2<\/sub>\n                    Suppresses Experimental Corneal Angiogenesis"],"prefix":"10.1177","volume":"20","author":[{"given":"Hailton B.","family":"Oliveira","sequence":"first","affiliation":[{"name":"The Schepens Eye Research Institute, Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Sakimoto","sequence":"additional","affiliation":[{"name":"The Schepens Eye Research Institute, Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel A.D.","family":"Javier","sequence":"additional","affiliation":[{"name":"The Schepens Eye Research Institute, Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitri T.","family":"Azar","sequence":"additional","affiliation":[{"name":"The Schepens Eye Research Institute, Harvard Medical School, Boston, MA"},{"name":"Department of Ophthalmology and Visual Sciences, University of Illinois, Chicago"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley J.","family":"Wiegand","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals Inc., Tarrytown, NY - USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep","family":"Jain","sequence":"additional","affiliation":[{"name":"The Schepens Eye Research Institute, Harvard Medical School, Boston, MA"},{"name":"Department of Ophthalmology and Visual Sciences, University of Illinois, Chicago"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Hong","family":"Chang","sequence":"additional","affiliation":[{"name":"The Schepens Eye Research Institute, Harvard Medical School, Boston, MA"},{"name":"Department of Ophthalmology and Visual Sciences, University of Illinois, Chicago"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,1,24]]},"reference":[{"key":"bibr1-112067211002000106","first-page":"264","volume":"104","author":"Azar D.T.","year":"2006","journal-title":"Trans Am Ophthalmol Soc"},{"key":"bibr2-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1038\/nature05249"},{"key":"bibr3-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506112103"},{"key":"bibr4-112067211002000106","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050834"},{"key":"bibr5-112067211002000106","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200868080-00002"},{"key":"bibr6-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708865104"},{"key":"bibr7-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2007.02.068"},{"key":"bibr8-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200701146"},{"key":"bibr9-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1016\/j.proghi.2007.06.001"},{"key":"bibr10-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2005.11.012"},{"key":"bibr11-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.10.013"},{"key":"bibr12-112067211002000106","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1463-1326.2007.00795.x","volume":"10","author":"Ejaz S.","year":"2008","journal-title":"Diabetes Obes Metab"},{"key":"bibr13-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E07-10-1068"},{"key":"bibr14-112067211002000106","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S1016-8478(23)15081-3","volume":"14","author":"Lee I.Y.","year":"2002","journal-title":"Mol Cells"},{"key":"bibr15-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.11.7151"},{"key":"bibr16-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-2444-5_34"},{"key":"bibr17-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2004.06.002"},{"key":"bibr18-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.7.1659"},{"key":"bibr19-112067211002000106","first-page":"1625","volume":"37","author":"Kenyon B.M.","year":"1996","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bibr20-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.01-1058"},{"key":"bibr21-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9355"},{"key":"bibr22-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1159\/000099253"},{"key":"bibr23-112067211002000106","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080452"},{"key":"bibr24-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-1380"},{"key":"bibr25-112067211002000106","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404272101"}],"container-title":["European Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/112067211002000106","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/112067211002000106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T19:35:01Z","timestamp":1777404901000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/112067211002000106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1177\/112067211002000106"],"URL":"https:\/\/doi.org\/10.1177\/112067211002000106","relation":{},"ISSN":["1120-6721","1724-6016"],"issn-type":[{"value":"1120-6721","type":"print"},{"value":"1724-6016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]}}}