{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:36:02Z","timestamp":1777581362830,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T00:00:00Z","timestamp":1584057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To report a case of primary choroidal lymphoma that was confirmed by polymerase chain reaction\u2013based clonality testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case report:<\/jats:title>\n                    <jats:p>A 50-year-old woman presented with unilateral progressive vision loss. Fundus examination and B-ultrasonography demonstrated diffuse choroidal thickening without vitritis. Pars plana vitrectomy and subretinal biopsy were performed, and histopathologic analysis revealed choroidal B-cell lymphoid hyperplasia without evidence of neoplasia. Extraocular extension was ruled out, and transitory improvement was observed with oral steroids. After 1-year follow-up, she was referred to our hospital and clonality testing was performed using the samples taken months before. First, we used a forensic DNA extraction kit, and then, a multiplex polymerase chain reaction was carried out using the IgH Rearrangements Molecular Analysis Kit. Clonal rearrangement was identified for the immunoglobulin heavy chain framework regions 1 and 2, and B-cell choroidal lymphoma was confirmed. The patient began treatment with intravitreal rituximab, but no response was observed. Finally, complete regression was achieved using external beam radiotherapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Polymerase chain reaction\u2013based clonality testing can be a valuable tool to confirm a choroidal lymphoproliferative process.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1120672120911333","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T05:00:46Z","timestamp":1584075646000},"page":"NP97-NP101","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Choroidal lymphoma diagnosed by polymerase chain reaction\u2013based clonality assessment"],"prefix":"10.1177","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4104-7780","authenticated-orcid":false,"given":"Antonio","family":"Hern\u00e1ndez-Pons","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Hospital Universitario Doctor Peset, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"G\u00f3mez-Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Genetics Laboratory, Department of Hematology, Hospital Universitario Doctor Peset, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Fern\u00e1ndez-Zarzoso","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hospital Universitario Doctor Peset, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mara","family":"Albert-Fort","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hospital Universitario Doctor Peset, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda","family":"Mart\u00ednez-Costa","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hospital Universitario Doctor Peset, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,3,13]]},"reference":[{"key":"bibr1-1120672120911333","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2013.09.004"},{"key":"bibr2-1120672120911333","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2013.06.046"},{"key":"bibr3-1120672120911333","doi-asserted-by":"publisher","DOI":"10.2147\/IMCRJ.S129171"},{"key":"bibr4-1120672120911333","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14025"},{"key":"bibr5-1120672120911333","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcjo.2011.12.017"},{"key":"bibr6-1120672120911333","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0000000000001026"},{"key":"bibr7-1120672120911333","unstructured":"Qiagen. 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