{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:26:58Z","timestamp":1784172418607,"version":"3.55.0"},"reference-count":10,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T00:00:00Z","timestamp":1597276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100008108","name":"Taipei Tzu Chi Hospital","doi-asserted-by":"publisher","award":["TCRD-TPE-108-8"],"award-info":[{"award-number":["TCRD-TPE-108-8"]}],"id":[{"id":"10.13039\/501100008108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purposes:<\/jats:title>\n                    <jats:p>A case of gonococcal keratoconjunctivitis rapidly diagnosed by a vaginal swab PCR Xpert\u00ae CT\/NG assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Case report.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>A 26-year-old woman presented to our emergency department with severe perilimbal stromal melting in both eyes and profuse purulent discharge for one day. Upon emergent ocular consultation, gonococcal keratoconjunctivitis was suspected. A vaginal swab was sent for rapid PCR Xpert\u00ae CT\/NG assay which reported positive Neisseria gonorrhoeae (NG) and Chlamydia Trachoma (CT) DNA detection within 90\u2009min. Due to the rapid diagnosis, adequate medical intervention with ceftriaxone injection was administered. Gonococcal keratitis with stromal melting was stabilized within 5\u2009days of presentation. The patient was discharged with complete epithelial healing by the 8th day. However, 10\u2009weeks after discharge, inadvertent rubbing of the left eye resulted in corneal perforation with iris prolapse. Lamellar keratoplasty with corneal patch graft was performed with amniotic membrane grafting. Xpert\u00ae CT\/NG assay was performed again with conjunctival swab for recurring mild eye discharge. Both NG and CT were negative. The patient thus stabilized with no further complications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Rapid stromal melting can occur with un-diagnosed or delayed diagnosis of gonorrhea with ocular involvement. Speedy and accurate diagnosis by the highly sensitive and specific Xpert\u00ae CT\/NG assay can provide early definite diagnosis for prompt treatment in prevention of gonococcal infection induced corneal perforations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1120672120951018","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T07:12:55Z","timestamp":1597302775000},"page":"NP19-NP23","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Rapid polymerase chain reaction test for the early diagnosis of gonococcal keratoconjunctivitis"],"prefix":"10.1177","volume":"32","author":[{"given":"Chia-Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Taipei Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6757-1647","authenticated-orcid":false,"given":"Elizabeth P","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Taipei Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, Taipei"},{"name":"School of Medicine, Tzu Chi University, Hua-Liang"},{"name":"Department of Ophthalmology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,8,13]]},"reference":[{"key":"bibr1-1120672120951018","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(87)33415-3"},{"key":"bibr2-1120672120951018","doi-asserted-by":"publisher","DOI":"10.1038\/sj.eye.6703051"},{"key":"bibr3-1120672120951018","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1965.00970040886033"},{"key":"bibr4-1120672120951018","first-page":"479","author":"Sarkis H","year":"2017","journal-title":"Cornea"},{"key":"bibr5-1120672120951018","first-page":"1","volume":"63","author":"Papp JR SJ","year":"2014","journal-title":"MMWR Recomm Rep"},{"key":"bibr6-1120672120951018","doi-asserted-by":"publisher","DOI":"10.1177\/0956462412472837"},{"key":"bibr7-1120672120951018","first-page":"1","volume":"59","author":"Workowski KA","year":"2010","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"bibr8-1120672120951018","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03461-12"},{"key":"bibr9-1120672120951018","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2014.871495"},{"key":"bibr10-1120672120951018","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12902"}],"container-title":["European Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1120672120951018","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1120672120951018","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1120672120951018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T19:46:16Z","timestamp":1777405576000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1120672120951018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,13]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1177\/1120672120951018"],"URL":"https:\/\/doi.org\/10.1177\/1120672120951018","relation":{},"ISSN":["1120-6721","1724-6016"],"issn-type":[{"value":"1120-6721","type":"print"},{"value":"1724-6016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,13]]}}}