{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:34:06Z","timestamp":1777581246314,"version":"3.51.4"},"reference-count":26,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Eurobio Scientific","award":["none"],"award-info":[{"award-number":["none"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To evaluate outcomes of ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK) with donor corneas preserved at 31\u00b0C in Cornea Syn\u00ae, a medium formulated with recombinant human serum albumin (rHSA) to replace foetal calf serum, and deswelled-transported in the xeno-free medium Cornea Trans\u00ae.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Prospective, multicentre, open-label study. We evaluated the endothelial cell loss (ECL) as the percentage variation of the endothelial cell density (ECD, cells\/mm\n                      <jats:sup>2<\/jats:sup>\n                      ) between 6 and 12 months after surgery, corneal transparency and thickness at 12 months, and adverse events within 12 months. Endothelial lenticules of mean 89\u2005\u03bcm, ECD \u2265 2300\u2005cells\/mm\n                      <jats:sup>2<\/jats:sup>\n                      , minimum signs of cell mortality or morphology alterations, were dissected by microkeratome in the eye bank, and grafted in patients \u2265 18 years without corneal neovascularisation, conjunctivalization, or blinking impairment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Thirty-five patients underwent UT-DSAEK, 3 showed primary failure, 1 late failure, and 2 skipped the 6-month visit. We analysed data from 29 patients, 27 with Fuchs endothelial corneal dystrophy (FECD) and 2 with pseudophakic bullous keratopathy (PBK). The median ECL between 6 and 12 months was 2.6% ( p\u2009=\u2009.054, CI 0 to 12.5) and the absolute mean (SD) was 158.4 (364.1) cells\/mm\n                      <jats:sup>2<\/jats:sup>\n                      . After 12 months, 96.5% of corneas were clear, with mean pachymetry of 585.9 (50.4) \u00b5m.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The ECL rate after UT-DSAEK match overall that observed in DSAEK or UT-DSAEK models of endothelial survival and the overall safety compared that reported for similar follow-up. Corneas maintained in Cornea Syn\u00ae and Cornea Trans\u00ae did not affect the ECD and functional outcomes of UT-DSAEK up to 12 months.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11206721221133141","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T03:20:47Z","timestamp":1667532047000},"page":"912-918","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Results of ultrathin Descemet stripping automated endothelial keratoplasty with donor corneas preserved in synthetic organ culture media"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2202-7477","authenticated-orcid":false,"given":"Adriano","family":"Fasolo","sequence":"first","affiliation":[{"name":"Research Unit, The Veneto Eye Bank Foundation, Venice, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Franch","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Ss Giovanni e Paolo Hospital, Venice, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Monterosso","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Dell\u2019Angelo Hospital, Venice, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Camposampiero","sequence":"additional","affiliation":[{"name":"Research Unit, The Veneto Eye Bank Foundation, Venice, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Chiara","family":"Frigo","sequence":"additional","affiliation":[{"name":"Department of Cardiological, Thoracic, Vascular and Public Health Sciences, Epidemiology and Public Health Section, University of Padova, Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Claude","family":"Amoureux","sequence":"additional","affiliation":[{"name":"Eurobio Scientific, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Fortier","sequence":"additional","affiliation":[{"name":"Eurobio Scientific, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Ponzin","sequence":"additional","affiliation":[{"name":"Research Unit, The Veneto Eye Bank Foundation, Venice, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"key":"bibr1-11206721221133141","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1097\/00003226-198404000-00002","volume":"3","author":"Pels E","year":"1984","journal-title":"Cornea"},{"key":"bibr2-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200304000-00015"},{"key":"bibr3-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0601"},{"key":"bibr4-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-1078"},{"key":"bibr5-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1111\/aos.13498"},{"key":"bibr6-11206721221133141","doi-asserted-by":"publisher","DOI":"10.14573\/altex.1705101"},{"key":"bibr7-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20720"},{"key":"bibr8-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-010-9263-3"},{"key":"bibr9-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000002097"},{"key":"bibr10-11206721221133141","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-017-9666-x"},{"key":"bibr11-11206721221133141","first-page":"L38\/56","volume":"9","author":"European Union","year":"2006","journal-title":"O J European Union"},{"key":"bibr12-11206721221133141","unstructured":"National Transplant Center (Centro Nazionale Trapianti, Italy). 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