{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T14:05:28Z","timestamp":1779199528398,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Ophthalmology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      To evaluate the\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      antimicrobial activity on the microbial flora of the eyelids and conjunctiva of the new medicated wipes LENIVA bio, soaked with an antiseptic solution containing natural extracts from citrus fruits (Biosecur\n                      <jats:sup>\u00ae<\/jats:sup>\n                      ),\n                      <jats:italic>Aloe vera<\/jats:italic>\n                      , and\n                      <jats:italic>Ruscus aculeatus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>in vitro<\/jats:italic>\n                      antimicrobial activity of the LENIVA bio antiseptic solution against bacteria and fungi commonly found in the periocular area, including antibiotic-resistant strains, was measured by agar diffusion and microdilution assays. The\n                      <jats:italic>in vivo<\/jats:italic>\n                      activity was measured on swabs collected from the eyelid rim and conjunctiva of 20 patients (13 males, 7 females; mean age 70.5 years) before and after a 4-day treatment with the product. Contralateral untreated eyes were used as controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>in vitro<\/jats:italic>\n                      analysis of inhibition halos in agar diffusion assays demonstrated that LENIVA bio is active against all tested germs, even when diluted.\n                      <jats:italic>In vivo<\/jats:italic>\n                      , the reduction in the total microbial counts obtained from both eyelid rim and conjunctival swabs following treatment with LENIVA bio wipes was statistically significant (respectively, mean reduction\u2009=\u20094530 CFU\/ml,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001; mean reduction\u2009=\u2009893 CFU\/ml,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0384).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      LENIVA bio wipes showed\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      antimicrobial activity against microorganisms that are potential pathogens of the eye and\/or commonly found in the periocular area.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11206721251332758","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T10:51:16Z","timestamp":1744714276000},"page":"1810-1817","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i>\n                    and\n                    <i>in vivo<\/i>\n                    antimicrobial activity of medicated wipes containing biosecur on the microbial Flora of the eyelid and conjunctiva"],"prefix":"10.1177","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2617-0289","authenticated-orcid":false,"given":"Giuseppe","family":"Giannaccare","sequence":"first","affiliation":[{"name":"Eye Clinic, Department of Surgical Sciences, University of Cagliari, Cagliari, Italy"},{"name":"Research in Ophthalmology Study Academy (R.O.S.A.), Reggio Calabria, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9346-9267","authenticated-orcid":false,"given":"Aldo","family":"Vagge","sequence":"additional","affiliation":[{"name":"IRCCS Ospedale Policlinico San Martino, Genoa, Italy"},{"name":"DiNOGMI, Clinica Oculistica, University of Genoa, Genoa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6419-6941","authenticated-orcid":false,"given":"Marco","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine, University of Ferrara, Ferrara, Italy"},{"name":"Ospedali Privati Forl\u00ec \u201cVilla Igea\u201d, Forl\u00ec, Italy"},{"name":"Istituto Internazionale per la Ricerca e Formazione in Oftalmologia, Forl\u00ec, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matilde","family":"Buzzi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Humanitas University, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1856-6363","authenticated-orcid":false,"given":"Rita","family":"Mencucci","sequence":"additional","affiliation":[{"name":"Eye Clinic, Department of Neurosciences, Psychology, Pharmacology, and Child Health, University of Florence, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3624-1776","authenticated-orcid":false,"given":"Massimo","family":"Lorusso","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ente Ecclesiastico Ospedale Generale Regionale F. 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