{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T02:16:23Z","timestamp":1777601783309,"version":"3.51.4"},"reference-count":80,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Evol Bioinform Online"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Cronobacter sakazakii<\/jats:italic>\n                      , a foodborne pathogen with a fatality rate of 33%, is a rod-shaped, Gram-negative, non-spore-forming bacterium responsible for causing meningitis, bacteremia, and necrotizing enterocolitis. Despite many unknown functions of hypothetical proteins in bacterial genomes, bioinformatic techniques have successfully annotated their roles in various pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>\n                      The aim of this investigation is to identify and annotate the structural and functional properties of a hypothetical protein (HP) from\n                      <jats:italic toggle=\"yes\">Cronobacter sakazakii<\/jats:italic>\n                      7G strain (accession no. WP_004386962.1, 277 residues) using computational tools.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Multiple bioinformatic tools were used to identify the homologous protein and to construct and validate its 3D structure. A 3D model was generated using SWISS-MODEL and validated using tools, developing a reliable 3D structure. The STRING and CASTp servers provided information on protein-protein interactions and active sites, identifying functional partners.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The putative protein was soluble, stable, and localized in the cytoplasmic membranes, indicating its biological activity. Functional annotation identified TagJ (HsiE1) within the protein, a member of the ImpE superfamily involved in the transport of toxins and a part of the bacterial type VI secretion system (T6SS). The 3-dimensional structure of this protein was validated through molecular docking involving 6 different compounds. Among these, ceforanide demonstrated the strongest binding scores, -7.5 kcal\/mol for the hypothetical protein and \u22127.2 kcal\/mol for its main template protein (PDB ID: 4UQX.1).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      Comparative genomics study suggests that the protein found in\n                      <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                      may be a viable therapeutic target because it seems distinctive and different from human proteins. The results of multiple sequence alignment (MSA) and molecular docking supported HP\u2019s potential involvement as a T6SS. These in silico results represent that the examined HP could be valuable for studying\n                      <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                      infections and creating medicines to treat\n                      <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                      -mediated disorders.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11769343251327660","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T10:49:32Z","timestamp":1743418172000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural and Functional Characterization of a Putative Type VI Secretion System Protein in\n                    <i>Cronobacter sakazakii<\/i>\n                    as a Potential Therapeutic Target: A Computational Study"],"prefix":"10.1177","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4730-3615","authenticated-orcid":false,"given":"Nurun Nahar","family":"Akter","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4282-2330","authenticated-orcid":false,"given":"Md. 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