{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:51:31Z","timestamp":1777503091968,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T00:00:00Z","timestamp":1752883200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T00:00:00Z","timestamp":1752883200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004607","name":"natural science foundation of guangxi province","doi-asserted-by":"publisher","award":["2024GXNSFBA010318"],"award-info":[{"award-number":["2024GXNSFBA010318"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012543","name":"youth science foundation of guangxi medical university","doi-asserted-by":"publisher","award":["GXMUYSF202313"],"award-info":[{"award-number":["GXMUYSF202313"]}],"id":[{"id":"10.13039\/501100012543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011828","name":"guangxi zhuang autonomous region health and family planning commission","doi-asserted-by":"publisher","award":["Z-A20230681"],"award-info":[{"award-number":["Z-A20230681"]}],"id":[{"id":"10.13039\/501100011828","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cancer Inform"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      Osteosarcoma (OS) is a rare and complex form of cancer that mostly affects children and adolescents. Pain is a common symptom for patients in OS which causes significant unhappiness and persistent aches. To date, there is minimal knowledge on the mechanisms underlying OS induced pain and few treatment options for patients. Previous genetic studies have demonstrated that the panel of four genes, artemin (\n                      <jats:italic toggle=\"yes\">ARTN<\/jats:italic>\n                      ), persephin (\n                      <jats:italic toggle=\"yes\">PSPN<\/jats:italic>\n                      ), glial cell line-derived neurotropic factor (\n                      <jats:italic toggle=\"yes\">GDNF<\/jats:italic>\n                      ), and neurturin (\n                      <jats:italic toggle=\"yes\">NRTN<\/jats:italic>\n                      ) are associated with the regulation of pain processing in OS and analgesic responses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>In the present study, by utilising a scRNA-seq OS dataset, we aimed to measure the gene expression levels of four pain related genes, and compare them between the different cell types in human OS tissues and cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Within a complex and diverse range of cell types in OS tissues, including osteoblastic OS cells, carcinoma associated fibroblasts (CAFs), B cells, myeloid cells 1, myeloid cells 2, NK\/T cells, plasmocytes,\n                      <jats:italic toggle=\"yes\">ARTN<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">NRTN<\/jats:italic>\n                      genes had the highest expression in Osteoblastic OS cells,\n                      <jats:italic toggle=\"yes\">GDNF<\/jats:italic>\n                      gene had a peak expression in carcinoma associated fibroblasts, and\n                      <jats:italic toggle=\"yes\">PSPN<\/jats:italic>\n                      gene in endothelial cells. In addition, all four genes showed deferential pattern of expression in 16 OS cell lines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Future studies should investigate the potential to target deferentially expressed pain-related genes in specific cell types of OS for therapeutic benefit to improve the quality of life for patients living with pain caused by OS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11769351251331508","type":"journal-article","created":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T11:13:10Z","timestamp":1752923590000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Single-Cell Transcriptome Analyses of Four Pain Related Genes in Osteosarcoma"],"prefix":"10.1177","volume":"24","author":[{"given":"Mesalie","family":"Feleke","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Perth, WA, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyingjie","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Perth, WA, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5554-6196","authenticated-orcid":false,"given":"Liang","family":"Mo","sequence":"additional","affiliation":[{"name":"Shenzhen University of Advanced Technology, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emel","family":"Rothzerg","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Perth, WA, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezhi","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinmin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenyu","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2021-8309","authenticated-orcid":false,"given":"Jiake","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The University of Western Australia, Perth, WA, Australia"},{"name":"Shenzhen University of Advanced Technology, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,7,19]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/jmp4020011"},{"key":"e_1_3_2_3_2","author":"Prater S","year":"2023","unstructured":"Prater S, McKeon B. Osteosarcoma. Treasure Island (FL): StatPearls Publishing. 2023.","journal-title":"Osteosarcoma"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-017-0467-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/15353702221088238"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/15353702221080131"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102097"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.6219"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12082130"},{"key":"e_1_3_2_10_2","volume-title":"High Yield Orthopaedics E-book","author":"Parvizi J.","year":"2010","unstructured":"Parvizi J. High Yield Orthopaedics E-book. 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Oncol Lett. 2019;17(3):3362-3370.","journal-title":"Oncol Lett"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0421-18.2018"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12860"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-13-245"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.21956"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/11769351231161478"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.709210"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11003"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16101788"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.08.005"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells12151996"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2017.10.010"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.64133"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2011.05.001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.06.007"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.05429.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2018.162"},{"issue":"9","key":"e_1_3_2_30_2","first-page":"10","article-title":"Pain management in metastatic bone disease: a literature review","volume":"10","author":"Ahmad I","year":"2018","unstructured":"Ahmad I, Ahmed MM, Ahsraf MF, et al. Pain management in metastatic bone disease: a literature review. 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