{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:03:16Z","timestamp":1793142196662,"version":"4.5.0"},"reference-count":53,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biomark&amp;#65533;Insights"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Myocardial infarction (MI), especially its adrenaline-induced myocardial injury, remains a leading cause of global mortality, often resulting in irreversible cardiac damage and heart failure due to stress cardiomyopathy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Ajuga bracteosa<\/jats:italic>\n                      Wall. ex Benth. (Lamiaceae). It is a medicinal plant used in the Himalayas for a long time in folk medicine for heart diseases, hypertension, and inflammatory diseases. Studies have reported the antioxidant and anti-inflammatory ability of its crude extracts. However, the active phytochemicals and their multi-target molecular mechanisms against adrenaline-induced MI remain unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>\n                      The current study investigated the predicted cardioprotective potential of\n                      <jats:italic toggle=\"yes\">A. bracteosa<\/jats:italic>\n                      , uses integrated network pharmacology, ADMET profiling, and molecular docking to investigate\n                      <jats:italic toggle=\"yes\">A. bracteosa<\/jats:italic>\n                      phytochemicals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Thirty bioactive compounds from the whole plant were identified. Of these, eight lead phytochemicals, including neo-clerodane diterpenoids and phytoecdysteroids, were subjected to ADMET screening. They passed the screening showing desirable drug-like properties. From network pharmacology analysis, 278 overlapping targets were identified between MI-associated genes and those in the ingredient database. The top three hub proteins were AKT1 (node degree 151), GAPDH (150), and TNF (149), respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>GO and KEGG enrichment analyses showed that these genes were primarily involved in the regulation of the apoptotic process, oxidant stress response, and the PI3K-Akt, TNF, and MAPK signaling pathways. Molecular docking (MOE 2019.01) results show that Ivain II showed the strongest binding affinity score with value of \u22129.42 kcal\/mol for GAPDH and TNF-\u03b1 through multiple hydrogen bonds, lipophilic contacts, and \u03c0-alkyl interactions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings provide the first computational mechanistic insight into the predicted multi-target cardioprotective potential of\n                      <jats:italic toggle=\"yes\">A. bracteosa<\/jats:italic>\n                      and identify Ivain II, 20-Hydroxyecdysone, and Ajugalactone as promising lead compounds warranting further experimental validation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11772719261472144","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:21:36Z","timestamp":1784730096000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Multi-Target Cardioprotective Potential of\n                    <i>Ajuga bracteosa<\/i>\n                    Phytochemicals: An Integrated Network Pharmacology and Molecular Docking Approach"],"prefix":"10.1177","volume":"21","author":[{"given":"Abdul","family":"Rehman","sequence":"first","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imran Ahmad","family":"Khan","sequence":"additional","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qasim Ali","family":"Ghauri","sequence":"additional","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4736-2546","authenticated-orcid":false,"given":"Maliha Khalid","family":"Khan","sequence":"additional","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Usman","sequence":"additional","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Umair","family":"Saleem","sequence":"additional","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Imran","family":"Ali Khan","sequence":"additional","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahsan","family":"Anjum","sequence":"additional","affiliation":[{"name":"MNS University of Agriculture"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6930-0145","authenticated-orcid":false,"given":"Hafiza","family":"Hamed","sequence":"additional","affiliation":[{"name":"Kabul University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.09.026"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.36948\/ijfmr.2025.v07i05.59272"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.22270\/jddt.v15i9.7366"},{"key":"e_1_3_6_5_2","first-page":"928","article-title":"Design, Synthesis, Antimalarial Activity and Docking Study of 7-Chloro-4-(2-(substituted benzylidene)hydrazineyl)quinolines","volume":"16","author":"Kalita J","year":"2020","unstructured":"Kalita J, Chetia D, Rudrapal M. 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