{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T18:48:41Z","timestamp":1793731721449,"version":"4.6.1"},"reference-count":39,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Post-Doctoral Research Grant","award":["Universitas Padjadjaran"],"award-info":[{"award-number":["Universitas Padjadjaran"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Bioinform Biol Insights"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Enzymatic reactions can be modulated by the incorporation of organic solvents, leading to alterations in enzyme stability, activity, and reaction rates. These solvents create a favorable microenvironment that enables hydrophobic reactions, facilities enzyme-substrate complex formation, and reduces undesirable water-dependent side reactions. However, it is crucial to understand the impact of organic solvents on enzymatic activity, as they can also induce enzyme inactivation. In this study, the enzymatic performance of Aspergillus oryzae \u03b1-amylase (Taka-amylase) in various organic solvents both experimentally and computationally was investigated. The results demonstrated that ethanol and ether sustain Taka-amylase activity up to 20% to 25% of the organic solvents, with ether providing twice the stability of ethanol. Molecular dynamics simulations further revealed that Taka-amylase has a more stable structure in ether and ethanol relative to other organic solvents. In addition, the analysis showed that the loop located near the active site in the AB-domain is a vulnerable site for enzyme destabilization when exposed to organic solvents. The ability of Taka-amylase to preserve the secondary loop structure in ether and ethanol contributed to the enzyme\u2019s activity. In addition, the solvent accessibility surface area of Taka-amylase is distributed throughout all enzyme structures, thereby contributing to the instability of Taka-amylase in the presence of most organic solvents.<\/jats:p>","DOI":"10.1177\/11779322241234767","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T06:27:10Z","timestamp":1713853630000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Enzymatic Performance of\n                    <i>Aspergillus oryzae<\/i>\n                    \u03b1-Amylase in the Presence of Organic Solvents: Activity, Stability, and Bioinformatic Studies"],"prefix":"10.1177","volume":"18","author":[{"given":"Khomaini","family":"Hasan","sequence":"first","affiliation":[{"name":"Research Centre for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia"},{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umi","family":"Baroroh","sequence":"additional","affiliation":[{"name":"Research Centre for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia"},{"name":"Department of Biotechnology, Indonesian School of Pharmacy, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indri Novia","family":"Madhani","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Universitas Jenderal Achmad Yani, Cimahi, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra Silmi","family":"Muscifa","sequence":"additional","affiliation":[{"name":"Research Centre for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia Tria","family":"Novianti","sequence":"additional","affiliation":[{"name":"Research Centre for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhamad","family":"Abidin","sequence":"additional","affiliation":[{"name":"Research Centre for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Yusuf","sequence":"additional","affiliation":[{"name":"Research Centre for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia"},{"name":"Department of Chemistry, Universitas Padjadjaran, Jatinangor, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toto","family":"Subroto","sequence":"additional","affiliation":[{"name":"Research Centre for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, Indonesia"},{"name":"Department of Chemistry, Universitas Padjadjaran, Jatinangor, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"key":"bibr1-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2009.11.010."},{"key":"bibr2-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201100534."},{"key":"bibr3-11779322241234767","doi-asserted-by":"publisher","DOI":"10.5936\/csbj.201209007."},{"key":"bibr4-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2016.10.003."},{"key":"bibr5-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1515\/boca-2015-0013."},{"key":"bibr6-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-016-0485-8."},{"key":"bibr7-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2016.07.016."},{"key":"bibr8-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-012-0071-7."},{"key":"bibr9-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2018.01.003."},{"key":"bibr10-11779322241234767","doi-asserted-by":"publisher","DOI":"10.3390\/catal8060238."},{"key":"bibr11-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2012.01.054."},{"key":"bibr12-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2010.02.003."},{"key":"bibr13-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1186\/s12575-016-0033-2."},{"key":"bibr14-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1007\/s00894-012-1507-z."},{"key":"bibr15-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2004.09.014."},{"key":"bibr16-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1039\/c4gc00117f."},{"key":"bibr17-11779322241234767","first-page":"75","volume":"53","author":"Baharum SN","year":"2003","journal-title":"Ann Microbiol"},{"key":"bibr18-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1002\/aic.690470318."},{"key":"bibr19-11779322241234767","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20215439."},{"key":"bibr20-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1590\/s1517-83822010000400004."},{"key":"bibr21-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-100868-3.00016-0."},{"key":"bibr22-11779322241234767","doi-asserted-by":"publisher","DOI":"10.2478\/s11756-008-0167-z."},{"key":"bibr23-11779322241234767","first-page":"1886","volume":"4","author":"Tiwari S","year":"2015","journal-title":"J Glob Biosci"},{"key":"bibr24-11779322241234767","first-page":"1865","volume":"6","author":"Saini R","year":"2017","journal-title":"J Pharmacogn Phytochem"},{"key":"bibr25-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/j.micromeso.2019.109700."},{"key":"bibr26-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(90)90030-T."},{"key":"bibr27-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a126476."},{"key":"bibr28-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/673173."},{"key":"bibr29-11779322241234767","author":"Case D","year":"2018","journal-title":"Amber 2018"},{"key":"bibr30-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21224."},{"key":"bibr31-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/b978-0-12-804492-6.00003-4."},{"key":"bibr32-11779322241234767","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1093\/oxfordjournals.jbchem.a134659","volume":"95","author":"Matsuura Y","year":"1984","journal-title":"J Biochem"},{"key":"bibr33-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/0144-8617(93)90008-R."},{"key":"bibr34-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)81432-6."},{"key":"bibr35-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(86)90440-6."},{"key":"bibr36-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1126\/science.6729453."},{"key":"bibr37-11779322241234767","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20102561."},{"key":"bibr38-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-010-0770-1."},{"key":"bibr39-11779322241234767","doi-asserted-by":"publisher","DOI":"10.1021\/cs500325p."}],"container-title":["Bioinformatics and Biology Insights"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/11779322241234767","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/11779322241234767","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/11779322241234767","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:55:54Z","timestamp":1777380954000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/11779322241234767"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":39,"alternative-id":["10.1177\/11779322241234767"],"URL":"https:\/\/doi.org\/10.1177\/11779322241234767","relation":{},"ISSN":["1177-9322","1177-9322"],"issn-type":[{"value":"1177-9322","type":"print"},{"value":"1177-9322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"article-number":"11779322241234767"}}