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Making an effective vaccine is one of the most important concerns for this disease. The new mRNA vaccine technology due to its accuracy and high efficiency has given promising results in various diseases. The objective of this research was to create a novel mRNA vaccine with multiple epitopes targeting Brucella melitensis. Seventeen antigenic proteins and their appropriate epitopes were selected with immunoinformatic tools and surveyed in terms of toxicity, allergenicity, and homology. Then, their presentation and identification by MHC cells and other immune cells were checked with valid tools such as molecular docking, and a multi-epitope protein was modeled, and after optimization, mRNA was analyzed in terms of structure and stability. Ultimately, the immune system\u2019s reaction to this novel vaccine was evaluated and the results disclosed that the designed mRNA construct can be an effective and promising vaccine that requires laboratory and clinical trials.<\/jats:p>","DOI":"10.1177\/11779322241272404","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T06:32:21Z","timestamp":1724999541000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Proteomics Exploration of\n                    <i>Brucella melitensis<\/i>\n                    to Design an Innovative Multi-Epitope mRNA Vaccine"],"prefix":"10.1177","volume":"18","author":[{"given":"Maryam","family":"Asadinezhad","sequence":"first","affiliation":[{"name":"Students Research Committee, Ilam University of Medical Sciences, Ilam, Iran"},{"name":"Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, 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