{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:19:06Z","timestamp":1777454346231,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"(Universal - MCTI\/CNPq\/ FNDCT","award":["479 No18\/2021 - Faixa B"],"award-info":[{"award-number":["479 No18\/2021 - Faixa B"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Bioinform Biol Insights"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Leishmaniasis is a neglected tropical disease caused by protozoa of the genus Leishmania, predominantly affecting populations with limited socioeconomic resources.\n                    <jats:italic>Leishmania (V.) braziliensis<\/jats:italic>\n                    is one of the primary etiological agents for cutaneous leishmaniasis (CL) in Brazil. This study aims to evaluate the interactions between IgG antibodies and 10 antigens derived from\n                    <jats:italic>L braziliensis<\/jats:italic>\n                    for diagnostic applications. These antigens were selected using in silico reverse vaccinology approaches, based on previous research conducted by our group.\n                    <jats:bold>Methods:<\/jats:bold>\n                    A total of 124 IgG antibody structures were retrieved from the SAbDab database. Antigen-antibody (Ag-Ab) complexes were subjected to molecular docking analyses using the SnugDock protocol implemented in the Rosetta platform. In parallel, enzyme-linked immunosorbent assays (ELISA) were performed to assess the diagnostic performance of the selected peptides in detecting active CL.\n                    <jats:bold>Results:<\/jats:bold>\n                    Peptides VIII, VI, V, and I showed the most favorable docking scores, indicating a higher predicted binding affinity with IgG. In ELISA assays, sensitivity values ranged from 0% to 96%, whereas specificity varied from 29% to 86%. Peptides III, IV, and V demonstrated the highest sensitivity, achieving values of 96%, 96%, and 94%, respectively.\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Considering both in silico and in vitro results, peptides IV and V corroborate significatively, demonstrating higher predicted affinity (more negative docking score values) with the set of antibodies (Ab) used in calculations.\n                  <\/jats:p>","DOI":"10.1177\/11779322251375244","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T04:56:56Z","timestamp":1759726616000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Antigenic Potential of Epitopes Derived From\n                    <i>Leishmania braziliensis<\/i>"],"prefix":"10.1177","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5123-4794","authenticated-orcid":false,"given":"NTC","family":"Costa","sequence":"first","affiliation":[{"name":"Medicinal Theoretical Chemistry Laboratory, Department of Pharmaceutical Sciences, Health Sciences Center, Universidade Federal de Pernambuco, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5188-043X","authenticated-orcid":false,"given":"AMS","family":"Pereira","sequence":"additional","affiliation":[{"name":"Immunopathology and Molecular Biology Laboratory, Department of Immunology, Aggeu Magalhaes Institute, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9325-8380","authenticated-orcid":false,"given":"CC","family":"Silva","sequence":"additional","affiliation":[{"name":"Immunopathology and Molecular Biology Laboratory, Department of Immunology, Aggeu Magalhaes Institute, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1347-8375","authenticated-orcid":false,"given":"ABX","family":"Silva","sequence":"additional","affiliation":[{"name":"Immunopathology and Molecular Biology Laboratory, Department of Immunology, Aggeu Magalhaes Institute, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1560-1290","authenticated-orcid":false,"given":"EO","family":"Souza","sequence":"additional","affiliation":[{"name":"Medicinal Theoretical Chemistry Laboratory, Department of Pharmaceutical Sciences, Health Sciences Center, Universidade Federal de Pernambuco, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5926-4252","authenticated-orcid":false,"given":"LFGR","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Medicinal Theoretical Chemistry Laboratory, Department of Pharmaceutical Sciences, Health Sciences Center, Universidade Federal de Pernambuco, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3624-474X","authenticated-orcid":false,"given":"MZ","family":"Hernandes","sequence":"additional","affiliation":[{"name":"Medicinal Theoretical Chemistry Laboratory, Department of Pharmaceutical Sciences, Health Sciences Center, Universidade Federal de Pernambuco, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7995-7360","authenticated-orcid":false,"given":"VRA","family":"Pereira","sequence":"additional","affiliation":[{"name":"Immunopathology and Molecular Biology Laboratory, Department of Immunology, Aggeu Magalhaes Institute, Recife, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,10,5]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2013.03.006"},{"key":"e_1_3_3_3_2","unstructured":"WHO\/PAHO. 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