{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:40:47Z","timestamp":1777574447705,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Environ\ufffdHealth\ufffdInsights"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Pseudomonas aeruginosa is a major cause of most opportunistic nosocomial infections in Ghana. The study sought to characterize P. aeruginosa isolates from market environments, poultry farms and clinical samples of patients from 2 district hospitals in the Ashanti region of Ghana. The genetic relatedness, plasmid profiles and antimicrobial sensitivity of the isolates were investigated. Culture based isolation and oprL gene amplification were used to confirm the identity of the isolates. Susceptibility testing was conducted using the Kirby Bauer disk diffusion method. Random whole genome typing of the P. aeruginosa strains was done using Enterobacterial repetitive-intergenic consensus based (ERIC) PCR assay. The most active agents against P. aeruginosa isolates were ceftazidime (90%), piperacillin (85%), meropenem, cefipeme and ticarcillin\/clavulanic acid (81.6%). The isolates were most resistant to gentamycin (69%), ciprofloxacin (62.1%), ticarcillin (56.3%) and aztreonam (25%). About 65% (n\u2009=\u200938) of the multi-drug resistant (MDR) P. aeruginosa isolates harbored 1 to 5 plasmids with sizes ranging from 2 to 116.8\u2009kb. A total of 27 clonal patterns were identified. Two major clones were observed with a clone showing resistance to all the test antipseudomonal agents. There is therefore a need for continued intensive surveillance to control the spread and development of resistant strains.<\/jats:p>","DOI":"10.1177\/11786302221078117","type":"journal-article","created":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T01:33:58Z","timestamp":1644888838000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Sensitivity Patterns, Plasmid Profiles and Clonal Relatedness of Multi-Drug Resistant\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Isolated From the Ashanti Region, Ghana"],"prefix":"10.1177","volume":"16","author":[{"given":"Hayford","family":"Odoi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Microbiology, School of Pharmacy, University of Health and Allied Sciences, Ho, Volta Region, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian Etsiapa","family":"Boamah","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2134-0034","authenticated-orcid":false,"given":"Yaw","family":"Duah Boakye","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelius Cecil","family":"Dodoo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, School of Pharmacy, University of Health and Allied Sciences, Ho, Volta Region, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Agyare","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,2,14]]},"reference":[{"key":"bibr1-11786302221078117","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S173867"},{"key":"bibr2-11786302221078117","unstructured":"World Health Organization. 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