{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:31:46Z","timestamp":1792517506225,"version":"4.5.0"},"reference-count":95,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Nutr Metab Insights"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>The current study aimed to conduct a phytochemical screening of commonly known fruit red grape ( Vitis vinifera L.) seed methanolic extract through gas chromatography and mass spectrometry (GC-MS) to identify the bioactive compounds responsible for its health benefits and evaluate the pharmacological potentialities of the extract and its fractions against oxidation, inflammation, pain, and diarrhea.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>The in vitro antioxidant, anti-inflammatory, and cytotoxic characteristics of methanolic extracts and various solvent fractions of V. vinifera were evaluated using the DPPH free radical scavenging assay, membrane stabilizing, and brine shrimp lethality bioassay. Furthermore, the study assessed the effects of crude extracts (200, 400, and 600\u2009mg\/kg of body weight) on pain relief and reduction of diarrhea in animals using methods such as tail immersion, the acetic acid-induced writhing technique, and a diarrheal mouse model induced with castor oil.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A total of 73 phytoconstituents were predominantly found in the seed extract based on the GC-MS analysis. Among the identified compounds, 9-octadecenamide (13.7%), and ( 9E,11E)-octadeca-9,11-dienoate (11.07%) are most abundant. Several notable constituents, such as gamma-sitosterol, stigmasterol, paromomycin, 4,6-cholestadienol, gamma-tocotrienol, 24-Propylidenecholest-5-en-3beta-ol, and alpha-tocopherol acetate, are also present. The methanolic extract of V. vinifera seed and its different solvent fractions showed promising antioxidant properties (IC\n                      <jats:sub>50<\/jats:sub>\n                      \u2009=\u20091.19-17.42\u2009\u00b5g\/mL) compared to the standard antioxidant butylated hydroxytoluene (IC\n                      <jats:sub>50<\/jats:sub>\n                      \u2009=\u200920.46\u2009\u00b5g\/mL). Aqueous soluble fraction exerted inhibition of nearly 50% heat-induced hemolysis compared to the standard acetylsalicylic acid (42%). Besides, all the tested doses (200, 400, and 600\u2009mg\/kg bw) of the crude extract showed significant ( P\u2009&lt;\u2009.05) analgesic and antidiarrheal effects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The current findings endorsed the health benefits of V. vinifera by revealing potent antioxidant, anti-inflammatory, analgesic, and antidiarrheal effects. Nevertheless, further in-depth analysis of the plant\u2019s chemical constituents and pharmacological effects on health is warranted for novel drug discovery from V. vinifera.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11786388241275100","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T01:19:59Z","timestamp":1726708799000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Chemical Profiling and Antioxidant, Anti-Inflammatory, Cytotoxic, Analgesic, and Antidiarrheal Activities from the Seeds of Commonly Available Red Grape (\n                    <i>Vitis vinifera<\/i>\n                    L.)"],"prefix":"10.1177","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9706-207X","authenticated-orcid":false,"given":"Md. Jamal","family":"Hossain","sequence":"first","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, State University of Bangladesh, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadija Rahman","family":"Lema","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, State University of Bangladesh, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Abdus","family":"Samadd","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, State University of Bangladesh, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumi","family":"Aktar","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, State University of Bangladesh, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad A.","family":"Rashid","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Abdullah","family":"Al-Mansur","sequence":"additional","affiliation":[{"name":"Institute of National Analytical Research and Services, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhanmondi, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,18]]},"reference":[{"key":"bibr1-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4051475"},{"key":"bibr2-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-42319-3_14"},{"key":"bibr3-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.902554"},{"key":"bibr4-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.112932"},{"key":"bibr5-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1177\/11786388231212116"},{"key":"bibr6-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/life12010060"},{"key":"bibr7-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18105379"},{"key":"bibr8-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00319"},{"key":"bibr9-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-75502-7_8"},{"key":"bibr10-11786388241275100","volume-title":"Food Science and Nutrition","author":"Radulescu C","year":"2023"},{"key":"bibr11-11786388241275100","doi-asserted-by":"publisher","DOI":"10.5772\/64821"},{"key":"bibr12-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-019-04113-w"},{"key":"bibr13-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-819541-3.00020-7"},{"key":"bibr14-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1021\/jo00054a013"},{"key":"bibr15-11786388241275100","volume-title":"Phytochemical Methods a Guide to Modern Techniques of Plant Analysis","author":"Harborne AJ.","year":"1998"},{"key":"bibr16-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3329\/dujps.v19i2.50636"},{"key":"bibr17-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-023-04185-4"},{"key":"bibr18-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27196404"},{"key":"bibr19-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e20100"},{"key":"bibr20-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-024-04337-0"},{"key":"bibr21-11786388241275100","first-page":"55","volume":"1","author":"Ezeja M","year":"2011","journal-title":"Ann Med Health Sci Res"},{"key":"bibr22-11786388241275100","volume":"6","author":"Rahman MM","year":"2023","journal-title":"Heal Sci Rep"},{"key":"bibr23-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27185957"},{"key":"bibr24-11786388241275100","first-page":"366","volume":"56","author":"Goswami D","year":"2001","journal-title":"Pharm"},{"key":"bibr25-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.02.034"},{"key":"bibr26-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1433.011"},{"key":"bibr27-11786388241275100","unstructured":"Tranylcypromine. In: LiverTox: Clinical and Research Information on Drug-Induced Liver Injury. National Institute of Diabetes and Digestive and Kidney Diseases; 2012. Accessed April 17, 2023. http:\/\/www.ncbi.nlm.nih.gov\/books\/NBK548572\/"},{"key":"bibr28-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1021\/jm00391a037"},{"key":"bibr29-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25010033"},{"key":"bibr30-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1211\/002235702760345473"},{"key":"bibr31-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8655-0"},{"key":"bibr32-11786388241275100","unstructured":"Sung W, Jung H, Lee I, Kim H, Lee D. Antimicrobial Effect of Furaneol Against Human Pathogenic Bacteria and Fungi. J Microbiol Biotechnol. Published online 2006. Accessed April 17, 2023.https:\/\/scholar.google.com\/scholar_lookup?title=Antimicrobial+Effect+of+Furaneol+Against+Human+Pathogenic+Bacteria+and+Fungi&author=Sung%2C+W.S.+%28Kyungpook+National+University%2C+Daegu%2C+Republic+of+Korea%29&publication_year=2006"},{"key":"bibr33-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1021\/ja01164a108"},{"key":"bibr34-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2012.01.015"},{"key":"bibr35-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/foods9050568"},{"key":"bibr36-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-018-0495-1"},{"key":"bibr37-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2021.103243"},{"key":"bibr38-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1002\/em.2850210405"},{"key":"bibr39-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2022.127277"},{"key":"bibr40-11786388241275100","first-page":"833","volume":"18","author":"Junior M","year":"2009","journal-title":"Food Sci Biotechnol"},{"key":"bibr41-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(97)00141-1"},{"key":"bibr42-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1080\/00958972.2019.1664738"},{"key":"bibr43-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2012.12.057"},{"key":"bibr44-11786388241275100","first-page":"639","volume":"61","author":"Koca\u00e7al\u0131\u015fkan I","year":"2006","journal-title":"Z Civilistische -forsch C"},{"key":"bibr45-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1080\/0972060X.2020.1829997"},{"key":"bibr46-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1080\/12538078.2003.10515420"},{"key":"bibr47-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c00269"},{"key":"bibr48-11786388241275100","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2018-0327"},{"key":"bibr49-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2341-2"},{"key":"bibr50-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1002\/ffj.1040"},{"key":"bibr51-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/B0-12-227555-1\/00502-5"},{"key":"bibr52-11786388241275100","first-page":"179","volume":"9","author":"Ibibia E","year":"2016","journal-title":"Gas"},{"key":"bibr53-11786388241275100","first-page":"2113","volume":"55","author":"Aoki T","year":"1991","journal-title":"Agric Biol Chem"},{"key":"bibr54-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1039\/c4fo00037d"},{"key":"bibr55-11786388241275100","first-page":"39","volume":"22","author":"Cynthia I","year":"2018","journal-title":"Res J Chem Env"},{"key":"bibr56-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2008.09.008"},{"key":"bibr57-11786388241275100","volume-title":"The Potentiation of the Antimicrobial Activities of Cutting Fluid Preservatives by EDTA","author":"Izzat IN","year":"1978"},{"key":"bibr58-11786388241275100","doi-asserted-by":"publisher","DOI":"10.4103\/pr.pr_102_17"},{"key":"bibr59-11786388241275100","doi-asserted-by":"publisher","DOI":"10.5530\/pj.2015.4.7"},{"key":"bibr60-11786388241275100","first-page":"179","volume":"83","author":"Matsubara H","year":"1997","journal-title":"J Hattori Bot Lab"},{"key":"bibr61-11786388241275100","first-page":"30","volume":"38","author":"Rajalakshmi K","year":"2016","journal-title":"Int J Pharm Sci"},{"key":"bibr62-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1007\/BF00380830"},{"key":"bibr63-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2008.07.001"},{"key":"bibr64-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3126\/botor.v11i0.21030"},{"key":"bibr65-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.120046"},{"key":"bibr66-11786388241275100","doi-asserted-by":"publisher","DOI":"10.5897\/JPP2015.0364"},{"key":"bibr67-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1021\/jf903573g"},{"key":"bibr68-11786388241275100","first-page":"223","volume-title":"Advances in Food and Nutrition Research","volume":"65","author":"Kim SK","year":"2012"},{"key":"bibr69-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/md16030094"},{"key":"bibr70-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-375083-9.00013-1"},{"key":"bibr71-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.08.011"},{"key":"bibr72-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.07.030"},{"key":"bibr73-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2005.06.005"},{"key":"bibr74-11786388241275100","doi-asserted-by":"crossref","first-page":"6519712","DOI":"10.1155\/2022\/6519712","volume":"2022","author":"Khalid A","year":"2022","journal-title":"Evid-Based Complement Altern Med ECAM"},{"key":"bibr75-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7209851"},{"key":"bibr76-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2013.07.002"},{"key":"bibr77-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27248709"},{"key":"bibr78-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e01575"},{"key":"bibr79-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9050373"},{"key":"bibr80-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1078\/0944-7113-00044"},{"key":"bibr81-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-971236"},{"key":"bibr82-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27134024"},{"key":"bibr83-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2019.06.026"},{"key":"bibr84-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcjd.2017.04.005"},{"key":"bibr85-11786388241275100","doi-asserted-by":"publisher","DOI":"10.2174\/138620706777698481"},{"key":"bibr86-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i25.3168"},{"key":"bibr87-11786388241275100","volume":"4","author":"Amee KNS","year":"2024","journal-title":"Pharmacol Res Prod"},{"key":"bibr88-11786388241275100","doi-asserted-by":"publisher","DOI":"10.4314\/tjpr.v16i3.8"},{"key":"bibr89-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.10.033"},{"key":"bibr90-11786388241275100","doi-asserted-by":"publisher","DOI":"10.3390\/antiox5020014"},{"key":"bibr91-11786388241275100","doi-asserted-by":"publisher","DOI":"10.4103\/2231-4040.137417"},{"key":"bibr92-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.03.025"},{"key":"bibr93-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.07.027"},{"key":"bibr94-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-03001-7"},{"key":"bibr95-11786388241275100","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(77)90171-X"}],"container-title":["Nutrition and Metabolic Insights"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/11786388241275100","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/11786388241275100","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/11786388241275100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T03:32:54Z","timestamp":1777692774000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/11786388241275100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":95,"alternative-id":["10.1177\/11786388241275100"],"URL":"https:\/\/doi.org\/10.1177\/11786388241275100","relation":{},"ISSN":["1178-6388","1178-6388"],"issn-type":[{"value":"1178-6388","type":"print"},{"value":"1178-6388","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"article-number":"11786388241275100"}}