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The compound was separated by reversed-phase chromatography using a mobile phase of 0.1\u2009mol\/L disodium hydrogen phosphate, 0.05\u2009mol\/L citric acid buffer (adjusted to pH 3.0) containing 3.0\u2009mmol\/L zinc acetate and 35\u2009mmol\/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336\u2009nm and an emission at 448\u2009nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112\u2009ng. The dipicolinic acid content of natto was 7.24\u2009\u00b1\u20090.54\u2009mg\/100\u2009g (wet weight, mean\u2009\u00b1\u2009standard deviation [SD], n\u2009=\u20096).<\/jats:p>","DOI":"10.1177\/1178646919852120","type":"journal-article","created":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T05:01:20Z","timestamp":1560920480000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Determination of Dipicolinic Acid in \u201cNatto\u201d by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate"],"prefix":"10.1177","volume":"12","author":[{"given":"Ken-ichi","family":"Mawatari","sequence":"first","affiliation":[{"name":"Department of Bioanalytical Chemistry, Faculty of Pharma-Science, Teikyo University, Tokyo, 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