{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:16:47Z","timestamp":1777936607243,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clin Med Insights Oncol"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from the nasopharynx with high morbidity and mortality in Southeast Asia and south of China. Roundabout guidance receptor 1 (ROBO1) can regulate axonogenesis (axon-like protrusion), which may play an important role in migration. However, the roles of ROBO1 in NPC have not been clarified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A comparative analysis employing the NPC transcriptome (GSE12452) and the axonogenesis-related genes (GO: 0050772) was performed. In total, 124 tissue blocks from patients primarily diagnosed as NPC (1993-2002) were examined using immunohistochemical staining. The connections between clinicopathological variables and protein immunoexpression were analyzed by Pearson\u2019s chi-square test. The Kaplan\u2013Meier method with a log-rank test was employed to plot survival curves. Multivariate analysis was performed using the Cox proportional hazards model to identify independent prognostic biomarker.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>According to transcriptome analysis, we found that ROBO1 is significantly highly expressed in NPC tissues compared with normal tissues. The immunohistochemistry (IHC) staining showed that high expression of ROBO1 was significantly related to primary tumor (T1T2 and T3T4) ( P\u2009=\u2009.024), nodal metastasis status (N0N1 and N2N3) ( P\u2009=\u2009.030), stage (I-II and III-IV) ( P\u2009=\u2009.019), and histological grade (keratinizing, non-keratinizing, and undifferentiated) ( P\u2009=\u2009.065). Importantly, NPC patients with high ROBO1 expression had poorer disease-specific survival (DSS) ( P\u2009=\u2009.0001), distal metastasis-free survival (DMeFS) ( P\u2009&lt;\u2009.0001), and local recurrence-free survival (LRFS) ( P\u2009=\u2009.0001) compared with NPC patients with low ROBO1 expression through the uni-\/multivariate and the Kaplan\u2013Meier survival analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Our report indicates that ROBO1 might be a potential prognostic biomarker for NPC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11795549221113244","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T07:28:23Z","timestamp":1658474903000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Roundabout Guidance Receptor 1 Is an Emerging Prognostic Biomarker for Nasopharyngeal Carcinoma"],"prefix":"10.1177","volume":"16","author":[{"given":"Sung-Wei","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Chi Mei Medical Center, Liouying"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Chieh","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Chi Mei Medical Center, Tainan"},{"name":"Department of Pharmacy, Chia-Nan University of Pharmacy and Science, Tainan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6197-9828","authenticated-orcid":false,"given":"Hong-Yue","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Chi Mei Medical Center, Tainan"},{"name":"Trans-Omic Laboratory for Precision Medicine, Chi Mei Medical Center, Tainan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin-Hwa","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Chi Mei Medical Center, Tainan"},{"name":"Trans-Omic Laboratory for Precision Medicine, Chi Mei Medical Center, Tainan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Fa","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Chi Mei Medical Center, Tainan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hsuan","family":"Kuo","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Internal Medicine, Chi-Mei Medical Center, Tainan"},{"name":"College of Pharmacy and Science, Chia Nan University, Tainan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nai-Wen","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Internal Medicine, Chi-Mei Medical Center, Tainan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu-Ju","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pet care and grooming, Chung Hwa University of Medical Technology, Tainan"},{"name":"Department of Clinical Pathology, Chi-Mei Medical Center, Tainan"},{"name":"Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Lun","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pet care and grooming, Chung Hwa University of Medical Technology, Tainan"},{"name":"Department of Otolaryngology, Chi Mei Medical Center, Tainan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,7,22]]},"reference":[{"key":"bibr1-11795549221113244","volume-title":"Nasopharyngeal Carcinoma (NPC, Lymphoepithelioma)","author":"Almomani 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