{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T23:41:29Z","timestamp":1779234089409,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Med Surg"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Preliminary studies have suggested that photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) can improve psoriasis and mycosis fungoides, two diseases where normal or malignant T cells play a central role.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>To determine if ALA-PDT induces apoptosis and caspase activation in Jurkat cells, a malignant T-cell line.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Jurkat cells were incubated with ALA in the presence of [\n                      <jats:sup>14<\/jats:sup>\n                      C]-thymidine followed by red light exposure. DNA fragmentation was measured 24 hours later with a DNA elution assay. The influence on DNA fragmentation of ALA concentration, time between ALA addition and light exposure, as well as light fluence were studied. The occurrence of oligonucleosome-sized DNA fragmentation was also studied with DNA electrophoresis. Caspase-3-like activity was monitored by measuring Ac-DEVD-AMC hydrolysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>DNA fragmentation as high as 88% was observed 24 hours after ALA-PDT. The percentage of DNA fragmentation increased with increasing doses of ALA, red light fluence, as well as longer incubation time with ALA. DNA fragmentation was observed as early as 3 hours after ALA-PDT. The presence of apoptosis after ALA-PDT was confirmed by DNA electrophoresis. An increase in caspase-3-like activities was detected following ALA-PDT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>ALA-PDT induces apoptosis and caspase-3-like activation in Jurkat cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/120347540100500103","type":"journal-article","created":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T15:32:01Z","timestamp":1499700721000},"page":"8-13","source":"Crossref","is-referenced-by-count":27,"title":["Photodynamic Therapy with 5-Aminolevulinic Acid Induces Apoptosis and Caspase Activation in Malignant T Cells"],"prefix":"10.1177","volume":"5","author":[{"given":"Faten","family":"Gad","sequence":"first","affiliation":[{"name":"Division of Dermatology, University of Montreal Hospital Centre, Notre-Dame Hospital, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Viau","sequence":"additional","affiliation":[{"name":"Division of Dermatology, University of Montreal Hospital Centre, Notre-Dame Hospital, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Bousbira","sequence":"additional","affiliation":[{"name":"Division of Dermatology, University of Montreal Hospital Centre, Notre-Dame Hospital, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Bertrand","sequence":"additional","affiliation":[{"name":"Division of Oncology, University of Montreal Hospital Centre, Notre-Dame Hospital, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Bissonnette","sequence":"additional","affiliation":[{"name":"Division of Dermatology, University of Montreal Hospital Centre, Notre-Dame Hospital, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,1,1]]},"reference":[{"key":"bibr1-120347540100500103","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(05)70458-7"},{"key":"bibr2-120347540100500103","doi-asserted-by":"publisher","DOI":"10.1016\/1011-1344(90)85083-9"},{"issue":"3","key":"bibr3-120347540100500103","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2340\/0001555574206207","volume":"74","author":"Boehncke WH","year":"1994","journal-title":"Acta Derm Venereol"},{"key":"bibr4-120347540100500103","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(08)81742-2"},{"key":"bibr5-120347540100500103","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)91883-X"},{"key":"bibr6-120347540100500103","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1992.tb04222.x"},{"key":"bibr7-120347540100500103","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90350-6"},{"key":"bibr8-120347540100500103","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1972.33"},{"issue":"23","key":"bibr9-120347540100500103","first-page":"6280","volume":"51","author":"Bertrand R","year":"1991","journal-title":"Cancer Res"},{"key":"bibr10-120347540100500103","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/oso\/9780199635696.003.0006","volume-title":"Cell growth and apoptosis; 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