{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:37:59Z","timestamp":1777513079754,"version":"3.51.4"},"reference-count":6,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1998,4,1]],"date-time":"1998-04-01T00:00:00Z","timestamp":891388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Med Surg"],"published-print":{"date-parts":[[1998,4]]},"abstract":"\n Background:<\/jats:title>\n Direct immunofluorescence (DIF) is a necessary examination tool for the diagnosis of pemphigus. The suction-blister-method splits the skin at the lamina lucida and it is possible with a scalpel to separate the entire epidermis from the dermis.<\/jats:p>\n <\/jats:sec>\n \n Objective:<\/jats:title>\n The study was to determine whether DIF is reliable on epidermal sheets separated using a suction apparatus.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n Thirteen patients were selected for this study: (nine with pemphigus vulgaris (PV), one with paraneoplastic pemphigus (PP), and three with pemphigus erythematosus (PE). Frozen epidermal sheets, separated from the dermis with a scalpel, were used as a substrate. Diagnosis with routine fluorescein isothiocyanate (FITC) antibodies was made.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n In all patients a pericellular deposition of IgG was evident and in eight of these patients a pericellular deposition of C3 was present. In two cases of PE and one of PP, the C3 deposits were also present in the lower part of basal keratinocytes.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n This diagnostic method without skin biopsy is easy to perform and, together with the histology and clinical aspects, could be a useful tool in the diagnosis of pemphigus. We recommend this method when the patient is allergic to local anaesthetics, the patient easily produces hypertrophic scars, or in follow-up of already biopsied patients.<\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/120347549800200406","type":"journal-article","created":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T15:45:58Z","timestamp":1499701558000},"page":"209-211","source":"Crossref","is-referenced-by-count":3,"title":["Direct Immunofluorescence Diagnosis of Pemphigus without Biopsy"],"prefix":"10.1177","volume":"2","author":[{"given":"Claudio","family":"Feliciani","sequence":"first","affiliation":[{"name":"Department of Dermatology, University \u201cG.D'Annunzio,\u201d Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saman Mohammad","family":"Pour","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University \u201cG.D'Annunzio,\u201d Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Toto","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University \u201cG.D'Annunzio,\u201d Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Coscione","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University \u201cG.D'Annunzio,\u201d Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Amerio","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University \u201cG.D'Annunzio,\u201d Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierluigi","family":"Amerio","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University \u201cG.D'Annunzio,\u201d Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1998,4,1]]},"reference":[{"key":"bibr1-120347549800200406","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1989.tb01423.x"},{"key":"bibr2-120347549800200406","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12543517"},{"key":"bibr3-120347549800200406","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(91)70151-Q"},{"key":"bibr4-120347549800200406","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12259692"},{"key":"bibr5-120347549800200406","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12374252"},{"key":"bibr6-120347549800200406","unstructured":"Feliciani C, Muzio M., Pour Mohammad S, \u201cSuction split\u201d as a routine method to differentiate epidermolysis bullosa acquisita from bullous pemphigoid. J Eur Acad Dermatol Venereol, (in print)."}],"container-title":["Journal of Cutaneous Medicine and Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/120347549800200406","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/120347549800200406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:46:58Z","timestamp":1777391218000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/120347549800200406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4]]},"references-count":6,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,4]]}},"alternative-id":["10.1177\/120347549800200406"],"URL":"https:\/\/doi.org\/10.1177\/120347549800200406","relation":{},"ISSN":["1203-4754","1615-7109"],"issn-type":[{"value":"1203-4754","type":"print"},{"value":"1615-7109","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,4]]}}}