{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:07:28Z","timestamp":1783591648330,"version":"3.55.0"},"reference-count":38,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mult Scler"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p>Background<\/jats:p>\n                  <jats:p>Blood\u2013brain barrier (BBB) breakdown is an early event in the pathogenesis of multiple sclerosis (MS). In a previous study we have found a direct stabilization of barrier characteristics after treatment of bovine brain capillary endothelial cells (BCECs) with human recombinant interferon-\u03b2-1a (IFN-\u03b2-1a) in an in vitro BBB model. In the present study we examined the effect of human recombinant IFN-\u03b2-1a on the barrier properties of BCECs derived from four different species including humans to predict treatment efficacy of IFN-\u03b2-1a in MS patients.<\/jats:p>\n                  <jats:p>Methods<\/jats:p>\n                  <jats:p>We used primary bovine and porcine BCECs, as well as human and murine BCEC cell lines. We investigated the influence of human recombinant IFN-\u03b2-1a on the paracellular permeability for 3H-inulin and 14C-sucrose across monolayers of bovine, human, and murine BCECs. In addition, the transendothelial electrical resistance (TEER) was determined in in vitro systems applying porcine and murine BCECS.<\/jats:p>\n                  <jats:p>Results<\/jats:p>\n                  <jats:p>We found a stabilizing effect on the barrier characteristics of BCECs after pretreatment with IFN-\u03b2-1a in all applied in vitro models: addition of IFN-\u03b2-1a resulted in a significant decrease of the paracellular permeability across monolayers of human, bovine, and murine BCECs. Furthermore, the TEER was significantly increased after pretreatment of porcine and murine BCECs with IFN-\u03b2-1a.<\/jats:p>\n                  <jats:p>Conclusion<\/jats:p>\n                  <jats:p>Our data suggest that BBB stabilization by IFN-\u03b2-1a may contribute to its beneficial effects in the treatment of MS. A human in vitro BBB model might be useful as bioassay for testing the treatment efficacy of drugs in MS.<\/jats:p>","DOI":"10.1177\/1352458508088940","type":"journal-article","created":{"date-parts":[[2008,5,27]],"date-time":"2008-05-27T23:09:00Z","timestamp":1211929740000},"page":"843-852","source":"Crossref","is-referenced-by-count":45,"title":["Interferon-\u03b2 stabilizes barrier characteristics of the                 blood\u2013brain barrier in four different species in vitro"],"prefix":"10.1177","volume":"14","author":[{"given":"J","family":"Kraus","sequence":"first","affiliation":[{"name":"Department of Neurology, Paracelsus Private Medical University and                         Salzburger Landesklinken, Christian-Doppler-Klinik, Salzburg, Austria;                         Department of Neurology, University Hospital of M\u00fcnster,                         M\u00fcnster, Germany,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Voigt","sequence":"additional","affiliation":[{"name":"Research Group for Multiple Sclerosis and Neuroimmunology, Department of                         Neurology, Justus-Liebig University of Giessen, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AM","family":"Schuller","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery, Johann-Wolfgang-Goethe                         University of Frankfurt, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Scholz","sequence":"additional","affiliation":[{"name":"Department of Trauma and Hand Surgery, Heinrich-Heine University of                         Duesseldorf, Duesseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KS","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases, Johns Hopkins University                         School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Schilling","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of M\u00fcnster,                         M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WR","family":"Sch\u00e4bitz","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of M\u00fcnster,                         M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Oschmann","sequence":"additional","affiliation":[{"name":"Research Group for Multiple Sclerosis and Neuroimmunology, Department of                         Neurology, Justus-Liebig University of Giessen, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Engelhardt","sequence":"additional","affiliation":[{"name":"Theodor Kocher Institute, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,7,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(00)01909-2"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/113.5.1477"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.45.7.1277"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)03334-0"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410390304"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(99)00185-9"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.46.4.907"},{"key":"atypb8","doi-asserted-by":"crossref","first-page":"299","DOI":"10.2165\/00063030-200519050-00003","volume":"19","author":"Chofflon 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