{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:24:59Z","timestamp":1778883899403,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2010,11,15]],"date-time":"2010-11-15T00:00:00Z","timestamp":1289779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mult Scler"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:p>Background: Progressive multifocal leukoencephalopathy (PML) is associated with natalizumab treatment in patients with multiple sclerosis (MS). It has been hypothesized that natalizumab mobilizes JC virus (JCV)-infected haematopoietic progenitor cells mediating viraemia and subsequently this disease.<\/jats:p>\n                  <jats:p>Objective: The objective of this study was to investigate peripheral haematopoietic progenitor cells for evidence of JCV DNA in MS patients treated with natalizumab.<\/jats:p>\n                  <jats:p>Methods: We assessed JCV and cytomegalovirus (CMV) DNA in magnetically separated CD34+ haematopoietic progenitor cells, peripheral blood mononuclear cells and plasma of 67 natalizumab-treated patients with MS and six PML patients.<\/jats:p>\n                  <jats:p>Results: Viral DNA was not detectable in CD34+ haematopoietic progenitor or peripheral blood mononuclear cells from any sample. Two plasma samples from patients with MS while undergoing natalizumab treatment were JCV-positive. In one case clinically manifest PML developed 8 months thereafter.<\/jats:p>\n                  <jats:p>Conclusions: Our findings do not support the hypothesis that natalizumab mobilizes JC virus-infected CD34+ cells from the bone marrow mediating JC viraemia. Notably, JC viraemia was detected in one patient with MS prior to developing clinical PML. This warrants further study.<\/jats:p>","DOI":"10.1177\/1352458510385834","type":"journal-article","created":{"date-parts":[[2010,11,15]],"date-time":"2010-11-15T22:19:31Z","timestamp":1289859571000},"page":"151-156","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["CD34+ progenitor cells mobilized by natalizumab are not a                     relevant reservoir for JC virus"],"prefix":"10.1177","volume":"17","author":[{"given":"Clemens","family":"Warnke","sequence":"first","affiliation":[{"name":"Department of Neurology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vsevolod","family":"Smolianov","sequence":"additional","affiliation":[{"name":"Department of Neurology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Dehmel","sequence":"additional","affiliation":[{"name":"Department of Neurology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Andr\u00e9e","sequence":"additional","affiliation":[{"name":"Institute for Virology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hartmut","family":"Hengel","sequence":"additional","affiliation":[{"name":"Institute for Virology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabian","family":"Zohren","sequence":"additional","affiliation":[{"name":"Department of Haematology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Arendt","sequence":"additional","affiliation":[{"name":"Department of Neurology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz","family":"Wiendl","sequence":"additional","affiliation":[{"name":"Department of Neurology,\r                Julius-Maximilians-University, W\u00fcrzburg, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rainer","family":"Haas","sequence":"additional","affiliation":[{"name":"Department of Haematology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Peter","family":"Hartung","sequence":"additional","affiliation":[{"name":"Department of Neurology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ortwin","family":"Adams","sequence":"additional","affiliation":[{"name":"Institute for Virology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd C","family":"Kieseier","sequence":"additional","affiliation":[{"name":"Department of Neurology,\r                Heinrich-Heine-University, D\u00fcsseldorf, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,11,15]]},"reference":[{"key":"bibr1-1352458510385834","doi-asserted-by":"publisher","DOI":"10.1086\/597117"},{"key":"bibr2-1352458510385834","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70226-9"},{"key":"bibr3-1352458510385834","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-10-186999"},{"key":"bibr4-1352458510385834","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(10)70028-4"},{"key":"bibr5-1352458510385834","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe020157"},{"key":"bibr6-1352458510385834","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0904267"},{"key":"bibr7-1352458510385834","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp0906248"},{"key":"bibr8-1352458510385834","doi-asserted-by":"crossref","unstructured":"Rudick RA, O'Connor PW, Polman CH, Goodman AD, Ray\n                    SS, Griffith NM, Jurgensen SA, Gorelik L, Forrestal F, Sandrock AW and Goelz S.\n                    Assessment of JC virus DNA in blood and urine from natalizumab-treated patients.\n                      Ann Neurol\n                      2010; 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