{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:16:22Z","timestamp":1777936582863,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2013,2,6]],"date-time":"2013-02-06T00:00:00Z","timestamp":1360108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mult Scler"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The StratifyJCV\u00ae test is a qualitative assay to classify MS patients as anti-JC virus (JCV) antibody positive or negative. Quantification of anti-JCV antibody levels in serum and cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients might add to the progressive multifocal leukoencephalopathy (PML) risk assessment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>The objective of this study is to test sera of patients in a quantitative anti-JCV antibody assay, and to compare the results with preexisting data from the StratifyJCV\u00ae test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Sera of a total of 175 MS patients and matched non-MS-controls were tested for anti-JCV antibodies using glutathione S-transferase-tagged-VP1 as antigen. Antibody reactivity was quantified in arbitrary units using human immunoglobulin as standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The comparison of our assay with StratifyJCV\u00ae showed good inter-assay agreement (kappa 0.6), and strong correlation for antibody reactivity ( r\n                      <jats:sup>2<\/jats:sup>\n                      = 0.94). Discordant samples had low-reactive positivity, and a higher proportion (13% vs. 4%) tested positive in the StratifyJCV\u00ae test only.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The method presented is a tool for the reliable quantification of anti-JCV antibodies, which demonstrates good agreement with results from StratifyJCV\u00ae. In contrast to StratifyJCV\u00ae, we pre-adsorbed all of the sera with BK virus (BKV) VP1 protein to reduce cross-reactivity. This step may account for a higher species-specificity of our assay. As such, our assay might be a promising additional tool for PML risk assessment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1352458513475489","type":"journal-article","created":{"date-parts":[[2013,2,7]],"date-time":"2013-02-07T00:59:21Z","timestamp":1360198761000},"page":"1137-1144","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["An assay to quantify species-specific anti-JC virus antibody levels in MS patients"],"prefix":"10.1177","volume":"19","author":[{"given":"Clemens","family":"Warnke","sequence":"first","affiliation":[{"name":"Department of Neurology, Medical Faculty, Heinrich-Heine-University, Germany"},{"name":"Department of Clinical Neuroscience, Karolinska Institutet, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Pawlita","sequence":"additional","affiliation":[{"name":"Infection and Cancer Program, German Cancer Research Center (DKFZ), Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Dehmel","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical Faculty, Heinrich-Heine-University, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Posevitz-Fejfar","sequence":"additional","affiliation":[{"name":"Department of Neurology, Inflammatory Disorders of the Nervous System and Neuro-oncology, University of Muenster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Peter","family":"Hartung","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical Faculty, Heinrich-Heine-University, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz","family":"Wiendl","sequence":"additional","affiliation":[{"name":"Department of Neurology, Inflammatory Disorders of the Nervous System and Neuro-oncology, University of Muenster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd C","family":"Kieseier","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical Faculty, Heinrich-Heine-University, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ortwin","family":"Adams","sequence":"additional","affiliation":[{"name":"Institute for Virology, Medical Faculty, Heinrich-Heine-University, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,2,6]]},"reference":[{"key":"bibr1-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1001\/archneurol.2010.161"},{"key":"bibr2-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70149-1"},{"key":"bibr3-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(10)70028-4"},{"key":"bibr4-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1586\/eci.11.85"},{"key":"bibr5-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1002\/ana.22128"},{"key":"bibr6-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1002\/ana.22606"},{"key":"bibr7-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1007\/s00115-011-3319-2"},{"key":"bibr8-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1107829"},{"key":"bibr9-1352458513475489","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3182583022"},{"key":"bibr10-1352458513475489","volume-title":"American 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