{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:45:12Z","timestamp":1787499912058,"version":"build-2736575974"},"reference-count":26,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T00:00:00Z","timestamp":1604534400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"deutsche forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"bundesministerium f\u00fcr bildung und forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008792","name":"novartis pharma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mult Scler"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Progressive multifocal leukoencephalopathy (PML) can in rare cases occur in natalizumab-treated patients with high serum anti-JCPyV antibodies, hypothetically due to excessive blockade of immune cell migration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Immune cell recruitment to the central nervous system (CNS) was assessed in relapsing-remitting multiple sclerosis (RRMS) patients stratified by low versus high anti-JCPyV antibody titers as indicator for PML risk.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Cerebrospinal fluid (CSF) cell counts of 145 RRMS patients were quantified by flow cytometry. Generalized linear models were employed to assess influence of age, sex, disease duration, Expanded Disability Status Scale (EDSS), clinical\/radiological activity, current steroid or natalizumab treatment, as well as anti-JCPyV serology on CSF cell subset counts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>While clinical\/radiological activity was associated with increased CD4, natural killer (NK), B and plasma cell counts, natalizumab therapy reduced all subpopulations except monocytes. With and without natalizumab therapy, patients with high anti-JCPyV serum titers presented with increased CSF T-cell counts compared to patients with low anti-JCPyV serum titers. In contrast, PML patients assessed before ( n\u2009=\u20092) or at diagnosis ( n\u2009=\u20095) presented with comparably low CD8 and B-cell counts, which increased after plasma exchange ( n\u2009=\u20094).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>High anti-JCPyV indices, which could be indicative of increased viral activity, are associated with elevated immune cell recruitment to the CNS. Its excessive impairment in conjunction with viral activity could predispose for PML development.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1352458520970103","type":"journal-article","created":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T06:15:58Z","timestamp":1604556958000},"page":"1491-1496","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["High anti-JCPyV serum titers coincide with high CSF cell counts in RRMS patients"],"prefix":"10.1177","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3717-5176","authenticated-orcid":false,"given":"Tilman","family":"Schneider-Hohendorf","sequence":"first","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Schulte-Mecklenbeck","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Ostkamp","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Janoschka","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Pawlitzki","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Luessi","sequence":"additional","affiliation":[{"name":"Department of Neurology, Focus Program Translational Neuroscience (FTN) and Immunotherapy (FZI), Rhine Main Neuroscience Network (rmn2), University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frauke","family":"Zipp","sequence":"additional","affiliation":[{"name":"Department of Neurology, Focus Program Translational Neuroscience (FTN) and Immunotherapy (FZI), Rhine Main Neuroscience Network (rmn2), University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven G","family":"Meuth","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Klotz","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4310-3432","authenticated-orcid":false,"given":"Heinz","family":"Wiendl","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catharina C","family":"Gross","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5494-9885","authenticated-orcid":false,"given":"Nicholas","family":"Schwab","sequence":"additional","affiliation":[{"name":"Department of Neurology with Institute of Translational Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,11,5]]},"reference":[{"key":"bibr1-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000003739"},{"key":"bibr2-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24286"},{"key":"bibr3-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020732"},{"key":"bibr4-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa044396"},{"key":"bibr5-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1177\/1352458517728814"},{"key":"bibr6-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2000.19269"},{"key":"bibr7-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24345"},{"key":"bibr8-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3182a351fb"},{"key":"bibr9-1352458520970103","doi-asserted-by":"crossref","first-page":"e1452","DOI":"10.1212\/WNL.0000000000008243","volume":"93","author":"Ryerson LZ","year":"2019","journal-title":"Neurology"},{"key":"bibr10-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.06.009"},{"key":"bibr11-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0711-8"},{"key":"bibr12-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007365"},{"key":"bibr13-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20858"},{"key":"bibr14-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/93.3.491"},{"key":"bibr15-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20140540"},{"key":"bibr16-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.63.10.1383"},{"key":"bibr17-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07053-9"},{"key":"bibr18-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awy151"},{"key":"bibr19-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz301"},{"key":"bibr20-1352458520970103","first-page":"11","author":"Steinbach K","year":"2019","journal-title":"Sci Transl Med"},{"key":"bibr21-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20181365"},{"key":"bibr22-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.09.007"},{"key":"bibr23-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1172\/JCI22833"},{"key":"bibr24-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000621"},{"key":"bibr25-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aar4526"},{"key":"bibr26-1352458520970103","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20151916"}],"container-title":["Multiple Sclerosis Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1352458520970103","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1352458520970103","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1352458520970103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T04:05:10Z","timestamp":1777694710000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1352458520970103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,5]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1177\/1352458520970103"],"URL":"https:\/\/doi.org\/10.1177\/1352458520970103","relation":{},"ISSN":["1352-4585","1477-0970"],"issn-type":[{"value":"1352-4585","type":"print"},{"value":"1477-0970","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,5]]}}}