{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:38:42Z","timestamp":1791319122302,"version":"4.3.1"},"reference-count":41,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vasc Med"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Impaired angiogenic response in peripheral artery disease (PAD) contributes to the progression of tissue ischemia, but methods to evaluate angiogenesis at the tissue level are limited. We describe a novel approach to measure angiogenesis and identify microRNA (miR)-gene pathways utilizing adipose tissue from patients with PAD.\n                    <jats:bold>Methods:<\/jats:bold>\n                    Patients with PAD undergoing infrainguinal bypass surgery or non-PAD control patients undergoing knee replacement surgery were recruited. Subcutaneous adipose tissue was obtained at the time of surgery. In patients with PAD, adipose tissue was taken from both the proximal and the distal ends of the surgical incision. Angiogenic capacity was measured and miR sequencing was performed. Differentially expressed miRs were defined by\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.01 and log\n                    <jats:sub>2<\/jats:sub>\n                    (fold change) &gt; 1 or &lt; \u20131. The miRs that correlated with angiogenic capacity and their gene targets were identified.\n                    <jats:bold>Results:<\/jats:bold>\n                    Participants with PAD (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 10) and control participants (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 5) were recruited. Capillary sprouting was impaired in distal (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    = 0.0014) but not proximal (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    = 0.12) PAD adipose tissue. In a subset of samples (controls:\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 4, PAD:\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 6), miR sequencing revealed 56 differentially expressed miRs in distal PAD. Six miRs with a correlation to impaired capillary sprouting and related to angiogenesis using Qiagen Ingenuity Pathway Analysis (IPA) software were identified (miRs 144-3p, 15b-5p, 18b-5p, 20b-5p, 454-3p, and 363-3p). These miRs are predicted to target regulators of angiogenesis including vascular endothelial growth factor A (VEGFA), phosphatase and tensin homologue (PTEN), and cyclin-dependent kinase inhibitor 1A (CDKN1A).\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    Evaluation of ischemic adipose tissue represents a novel approach to gain insight into impaired angiogenesis in PAD. Integration with miR sequencing and target analysis has the potential to identify novel pathways of impaired angiogenesis in PAD.\n                  <\/jats:p>","DOI":"10.1177\/1358863x251359542","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T06:20:15Z","timestamp":1756189215000},"page":"537-544","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Novel microRNA pathways of impaired angiogenesis in human peripheral artery disease adipose tissue"],"prefix":"10.1177","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3513-4263","authenticated-orcid":false,"given":"Ross A","family":"Okazaki","sequence":"first","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed HM","family":"Rizvi","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Bret\u00f3n-Romero","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M","family":"Weisbrod","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoheng","family":"Li","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6811-4384","authenticated-orcid":false,"given":"Melissa G","family":"Farb","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alik","family":"Farber","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Vascular and Endovascular Surgery, Boston Medical Center, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5504-5589","authenticated-orcid":false,"given":"Naomi M","family":"Hamburg","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine, Section of Vascular Biology, Whitaker Cardiovascular Institute, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.124.320195"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.119.040672"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(02)02524-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000093398.16124.29"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2006.17.683"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.009407"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-019-0344-x","article-title":"MicroRNA-148a\/b-3p regulates angiogenesis by targeting neuropilin-1 in endothelial cells","volume":"51","author":"Kim H","year":"2019","unstructured":"Kim H, Ko Y, Park H, et al MicroRNA-148a\/b-3p regulates angiogenesis by targeting neuropilin-1 in endothelial cells. 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